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甘蓝型油菜磷转运蛋白BnaPT48的功能研究

张雯 ,李玉,王 创,石磊,丁广大*   

  1. 华中农业大学资源与环境学院 / 微量元素研究中心 / 农业农村部长江中下游耕地保育重点实验室, 湖北武汉 43007
  • 收稿日期:2025-04-18 修回日期:2025-08-13 接受日期:2025-08-13 网络出版日期:2025-08-19
  • 基金资助:
    本研究由农业生物育种国家科技重大专项(2023ZD04072)资助。

Functional study of the phosphate transporter protein BnaPT48 in Brassica napus

ZHANG Wen,LI Yu,WANG Chuang,SHI Lei,DING Guang-Da*#br#

#br#
  

  1. College of Resources and Environment, Huazhong Agricultural University / Microelement Research Center / Key Laboratory of Arable Land Conservation in the Middle and Lower Reaches of Yangtze River, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China
  • Received:2025-04-18 Revised:2025-08-13 Accepted:2025-08-13 Published online:2025-08-19
  • Supported by:
    This study was supported by the National Key R&D Program for Agricultural Biological Breeding (2023ZD04072).

摘要:

磷是植物生长发育必需的大量营养元素之一。油菜作为我国重要的油料作物,具有需磷多且对缺磷敏感的特性。本研究以在甘蓝型油菜根系中表达丰度高且受缺磷诱导的基因BnaPT48为研究对象,通过分析其基因表达模式、蛋白亚细胞定位及遗传材料的表型等,揭示该基因在油菜磷吸收中的作用。研究发现BnaPT48定位于细胞膜,且在根系各组织中受缺磷显著诱导表达。BnaPT48能回补拟南芥磷吸收突变体(atpt1/2)的表型,表明BnaPT48能促进植物的磷吸收。在拟南芥中过表达BnaPT48能促进植株在正常磷和低磷条件下的生长。在油菜中过表达BnaPT48,低磷处理后显著增加地上部干重,降低子叶中的无机磷浓度,促进子叶中叶绿素的降解和子叶衰老,根系无机磷浓度在正常磷时显著增加。本研究揭示了BnaPT48在调控植物磷吸收中的作用,为甘蓝型油菜磷效率的遗传改良提供了理论基础和基因资源。

关键词: 甘蓝型油菜, BnaPT48, 转运蛋白, 低磷胁迫, 磷浓度, 磷吸收

Abstract:

Phosphorus (P) is an essential macronutrient required for plant growth and development. Rapeseed (Brassica napus), a major oilseed crop in China, is characterized by a high demand for P and strong sensitivity to P deficiency. This study investigates the gene BnaPT48, which is highly expressed in the root system of Brassica napus and is strongly induced under P-deficient conditions. Through analyses of its expression pattern, protein subcellular localization, and phenotypes of transgenic materials, the role of BnaPT48 in P uptake was elucidated. BnaPT48 was found to localize to the plasma membrane and was significantly upregulated by P deficiency in various root tissues. The BnaPT48 protein complemented the P uptake defect of the Arabidopsis thaliana mutant atpt1/2, indicating its functional role in enhancing P acquisition. Overexpression of BnaPT48 in A. thaliana promoted plant growth under both normal and low P conditions. In rapeseed, overexpression of BnaPT48 significantly increased the shoot dry weight under low P treatment, reduced inorganic P concentration in cotyledons, accelerated chlorophyll degradation and cotyledon senescence, and increased inorganic P accumulation in roots under normal P conditions. These findings reveal the function of BnaPT48 in regulating phosphate uptake and redistribution, and provide a theoretical foundation and genetic resource for improving P use efficiency in B. napus through genetic approaches.


Key words: Brassica napus, BnaPT48, transport protein, low phosphorus stress, P concentration, P absorption

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