欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2007, Vol. 33 ›› Issue (02): 317-321.

• 研究论文 • 上一篇    下一篇

用SSR技术和混合取样方法估算玉米群体间的遗传距离

侯本军1;王铁固1,2;陈彦惠1,*;吴连成1;库丽霞1   

  1. 1 河南农业大学农学院，河南郑州450002；2 郑州大学离子束生物工程重点实验室，河南郑州450052
  • 收稿日期:2006-01-04 修回日期:1900-01-01 出版日期:2007-02-12 网络出版日期:2007-02-12
  • 通讯作者: 陈彦惠

DNA Sampling Strategy and Calculation of Genetic Distance between Maize (Zea mays L.) Populations

HOU Ben-Jun1,WANG Tie-Gu12,CHEN Yan-Hui1*,WU Lian-Cheng1,KU Li-Xia1   

  1. 1 Agronomy College, Henan Agricultural University, Zhengzhou 450002, Henan; 2Ion Beam Biotechnology Key Laboratory, Zhengzhou University, Zhengzhou 450052, Henan, China
  • Received:2006-01-04 Revised:1900-01-01 Published:2007-02-12 Published online:2007-02-12
  • Contact: CHEN Yan-Hui

PDF

1254

被引次数

5

摘要：

The factors affecting the binding characteristics of GBSS with starch granule were studied using a wheat (Triticum aestivum) cultivar Chinese Spring with different temperature treatments. After sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, we got GBSS concentration by coomassie brilliant blue G-250(CBB G-250) method. The results showed that the temperature affected the binding of GBSS and starch granule. In 50–80℃, the concentration of GBSS unbound from starch increased with the temperature rising, which the maximal concentration was 11.361 μg mL-1 at 80℃, while that was reduced when the temperature was 85–95℃. Furthermore, Mg2+ could also affect the quantity of GBSS unbound from starch. When Mg2+ concentration was lower than 1.75 mmol L-1, the concentration of GBSS unbound increased. The lower Mg2+ concentration, the higher the concentration of GBSS unbound. However, when Mg2+ concentration was higher than 2.5 mmol L-1, it could restrain GBSS unbinding. These results showed that GBSS bound starch granule by non-covalent bonds. The best GBSS extracting conditions were 55 mmol L-1 Tris-HCl (pH 6.8), 0.75 mmol L-1 MgCl2, 2.3% SDS, 5% 2-ME and 10% glycerol, 15 min in boiling water, or 55 mmol L-1 Tris-HCl (pH 6.8), 0.75 mmol L-1 MgCl2, 2.3% SDS, 5% 2-ME and 10% glycerol, 80℃ 30 min. The results are helpful to investigate 3-D structure of biological activated GBSS and mechanism of GBSS binding with starch granule.

关键词: SSR, 玉米, 遗传距离, 遗传多样性, 取样方法

Abstract:

SSR is a good molecule marker with broad application. Bulk sampling is feasible to analyze genetic diversity of maize population by SSR. It is necessary to study influence of genetic distance as using bulk sampling. In present study, the genetic distances between two populations were compared and analyzed by 50 pairs of SSR primers, checked by inbred lines Huangzao 4 and Mo17, and using 60 individuals DNA samples of Golden Queen and Yuzong 5 maize populations with six treatments (the individual DNA samples, the mixed DNA samples with 5 individuals, 10 individuals, 15 individuals, 20 individuals, and 30 individuals, respectively). The results indicated that using the individual DNA samples can detect the alleles with very low frequencies, and make the detected allele number decreased gradually and the frequency of missing alleles increased with the increase of mixed individuals in the treatmen. The results of the genetic distance between two populations calculated by three models suggested that the genetic distances GD, GD(J) and GD(N) showed a gradually increasing trend with the increasing of the mixed individual number, but the increasing extent of GD(J) and GD(N) was wider. There was an obvious deviation between GD(J), GD(N) from mixed samples and the GD from 60 individual DNA samples based on allele frequency. The results and the comparison of the genetic distances based on different calculation methods suggested that the 10 individuals’ DNA mixture is the best sampling strategy when genetic diversity and polymorphism in maize populations are studied by SSR.
If the Nei’s genetic distance GD(N) and Jaccord’s genetic distance GD(J) are calculated according to 0, 1 data, (GD(N)+GD(J))/2 will be calculated close to the genetic distance by allele frequency of individuals. Gene frequency can be studied by using capillary electrophoresis and SSR fluorescence detection. There will be a broad application prospect about bulk sampling strategy with SSR marker.

Key words: SSR, Maize, Genetic distance, Genetic diversity, Sampling method

[1] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[2] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[3] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[4] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[5] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[6] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[7] 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[8] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[9] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[10] 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[11] 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
[12] 徐建霞, 丁延庆, 曹宁, 程斌, 高旭, 李文贞, 王若若, 王磊, 张立异. 397份高粱种质资源在贵州表型多样性分析及综合评价[J]. 作物学报, 2026, 52(4): 1073-1087.
[13] 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021.
[14] 田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[15] 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2