欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (09): 1563-1568.doi: 10.3724/SP.J.1006.2008.01563

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜油酸脱氢酶基因(fad2)多个拷贝的发现及分析

肖钢1,2;张宏军1,2;彭琪1;官春云1,2,*   

  1. 1 湖南农业大学作物基因工程湖南省重点实验室, 湖南长沙 410128; 2 湖南农业大学油料作物研究所 / 国家油料作物改良中心湖南分中心, 湖南长沙 410128
  • 收稿日期:2007-12-04 修回日期:1900-01-01 出版日期:2008-09-12 网络出版日期:2008-09-12
  • 通讯作者: 官春云

Screening and Analysis of Mutiple Copy of Oleate Desaturase Gene (fad2) in Brassica napus

XIAO Gang12,ZHANG Hong-Jun12,PENG Qi1,GUAN Chun-Yun12*   

  1. 1 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, Hunan; 2 Oil Crops Research Institute, Hunan Agricultural University / Hunan Branch of National Oil Crops Improvement Center, Changsha 410128, Hunan, China
  • Received:2007-12-04 Revised:1900-01-01 Published:2008-09-12 Published online:2008-09-12
  • Contact: GUAN Chun-Yun

PDF

2321

被引次数

35

摘要： 以甘蓝型油菜湘油15为材料, 随机挑选56个来自基因组的fad2基因克隆、47个来自幼苗整株的fad2基因cDNA克隆和9个授粉27 d后种子中fad2 cDNA克隆进行双向测序。基因组中56个fad2序列的碱基同源性为91.0%~99.9%, 从中得到11个差异序列, 即11个不同的fad2基因拷贝。将其翻译成氨基酸序列发现, 6个拷贝在编码区中出现多个终止密码子, 另外5个的同源性为90.60%~99.74%, 与47个来自幼苗整株的fad2基因cDNA序列进行比较, 发现fad2基因没有内含子。从种子中的9个fad2 cDNA克隆序列中找到2个有差异的cDNA, 它们的编码区中没有终止密码子, 说明在种子中有多个fad2基因表达。基因组中的11个拷贝根据同源性可分成两组, 命名为fad2I和fad2II。RT-PCR分析发现在授粉27 d的种子中fad2I有较强表达, fad2II没有表达; 但在叶片中两者都有表达。

关键词: 甘蓝型油菜, 油酸脱氢酶基因, 基因拷贝数, 序列差异引物

Abstract: Rapeseed oil is primarily composed of palmitic, stearic, oleic, linoleic and linolenic fatty acids. Delta-12 desaturase (FAD2) in plants converts oleic acid (18:1) to linoleic acid (18:2) by inserting a double bond at the delta-12 position. Fatty acid desaturase-2 gene (fad2) encodes delta-12 desaturase that functions in the endoplasmic reticulum. Fifty-six fad2 DNA clones and nine fad2 seed cDNA clones of B. napus cv. Xiangyou 15 were randomly selected and sequenced in this study. These 56 DNA sequences share 91.0-99.9% identity in nucleotides. Through combination of identical sequences, 11 different sequences were found, of which each represented various copy of fad2 gene. Deduced amino acid sequences revealed that many stop codons occurred in the coding region of six copies, the other five copies shared 90.60-99.74% amino-acid identity. Two differential cDNA were found from nine fad2 seed cDNA sequence, no stop codons in the coding region, revealing that more than one fad2 gene express in developing seeds, and oleic acid content is controlled by multigene. The eleven copies in genome could be divided into two groups based on their homology, and designated as fad2I and fad2II, respectively. RT-PCR analysis in seeds at twenty-seven days after pollination showed that fad2I expressed strongly in seeds, and no expression of fad2II was found, but both of them expressed in leaves.

Key words: Brassica napus, Oleate desaturase, Gene copy number, Sequence-specific primer

[1] 马毅娜, 吴晓明玉, 李藕琪, 王圆, 陈丽, 张盈川, 赵伦, 文静, 傅廷栋, 沈金雄. Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究[J]. 作物学报, 2026, 52(2): 349-362.
[2] 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110.
[3] 夏琦, 郭滢, 王坤美, 王思忆, 巨建业, 彭雅雯, 刘忠松, 夏石头. 甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究[J]. 作物学报, 2025, 51(5): 1189-1197.
[4] 王晓琳, 刘忠松, 康雷, 杨柳. 甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析[J]. 作物学报, 2025, 51(4): 888-899.
[5] 张琴, 戴成, 马朝芝. 生长素响应报告基因转化甘蓝型油菜及各组织GUS动态信号分析[J]. 作物学报, 2025, 51(3): 667-675.
[6] 孙程明, 周晓婴, 陈锋, 张维, 王晓东, 彭琦, 郭月, 高建芹, 胡茂龙, 付三雄, 张洁夫. 长链非编码RNA (lncRNA)在甘蓝型油菜分枝角度调控中的功能分析与预测[J]. 作物学报, 2025, 51(3): 559-567.
[7] 黄绒, 周渠晨, 陈楚铭, 罗倩, 易东, 杜常欢, 黄祥宇, 盛锋, 杜雪竹. 过表达BnNRT2.3-like对油菜氮素吸收利用及产量的影响[J]. 作物学报, 2025, 51(12): 3184-3197.
[8] 巨建业, 杨柳, 陈浩, 康雷, 夏石头, 刘忠松. 单细胞核转录组分析揭示油菜种皮分化过程和种子颜色差异原因[J]. 作物学报, 2025, 51(11): 2860-2874.
[9] 张雯, 李玉, 王创, 石磊, 丁广大. 甘蓝型油菜磷转运蛋白BnaPT48的功能研究[J]. 作物学报, 2025, 51(11): 2983-2995.
[10] 胡志康, 舒雨, 王会, 杨莹莹, 廖俊宇, 刘佳, 成洪涛, 郭晨, 张园园, 刘胜毅, 胡琼, 梅德圣, 李超. 甘蓝型油菜苗期耐碱性种质综合鉴定与评价[J]. 作物学报, 2025, 51(10): 2681-2692.
[11] 王晨, 贺丹, 姚敏, 邱萍, 何昕, 熊兴华, 康雷, 刘忠松, 钱论文. 基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证[J]. 作物学报, 2025, 51(10): 2693-2704.
[12] 徐林珊, 郜耿东, 王宇, 王家星, 杨吉招, 武亚瑞, 张宵寒, 常影, 李真, 谢雄泽, 龚德平, 王晶, 葛贤宏. 甘蓝型油菜漆酶基因家族成员表达模式及与茎秆抗折力的关联分析[J]. 作物学报, 2025, 51(1): 134-148.
[13] 李嘉欣, 黄莹, 吴潞梅, 赵伦, 易斌, 马朝芝, 涂金星, 沈金雄, 傅廷栋, 文静. 甘蓝型油菜BnaSLY1基因进化分析及功能研究[J]. 作物学报, 2025, 51(1): 44-57.
[14] 钟元, 朱天宇, 戴成, 马朝芝. 耐亚磷酸盐除草剂转基因油菜的创建和抗性评价[J]. 作物学报, 2024, 50(5): 1158-1171.
[15] 曹松, 姚敏, 任睿, 贾元, 向星汝, 李文, 何昕, 刘忠松, 官春云, 钱论文, 熊兴华. 转录组结合区域关联分析挖掘油菜含油量积累的候选基因[J]. 作物学报, 2024, 50(5): 1136-1146.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2