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作物学报 ›› 2014, Vol. 40 ›› Issue (05): 823-829.doi: 10.3724/SP.J.1006.2014.00823

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SSR分子指纹和商品信息构建水稻品种身份证

陆徐忠1,倪金龙1,李莉1,汪秀峰1,马卉1,张小娟1,2,杨剑波1,*   

  1. 本研究由国家科技支撑计划项目(2012BAD07B01)，国家自然科学基金青年科学基金项目(31101204)和安徽省农业科学院院长青年创新基金项目(11B0106)资助。
  • 收稿日期:2013-09-23 修回日期:2013-12-12 出版日期:2014-05-12 网络出版日期:2014-02-14
  • 通讯作者: 杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212
  • 作者简介:杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212
  • 基金资助:

    本研究由国家科技支撑计划项目(2012BAD07B01)，国家自然科学基金青年科学基金项目(31101204)和安徽省农业科学院院长青年创新基金项目(11B0106)资助。

Construction of Rice Variety Based on ID Used SSR Fingerprint and Commodity Information

LU Xu-Zhong1,NI Jin-Long1,LI Li1,WANG Xiu-Feng1,MA Hui1,ZHANG Xiao-Juan1,2,YANG Jian-Bo1,*   

  1. 1 Institute of Rice Research, Anhui Academy of Agricultural Sciences, Hefei 230031, China; 2.Institute of Cotton Research, Anhui Academy of Agricultural Sciences, Anqing 246003, China
  • Received:2013-09-23 Revised:2013-12-12 Published:2014-05-12 Published online:2014-02-14
  • Contact: 杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212
  • About author:杨剑波, E-mail: yjianbo@263.net, Tel: 0551-62160212

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摘要：

为方便水稻种子质量的追溯和管理，提出基于SSR分子指纹和商品信息构建水稻品种身份证的新思路。在前期研究基础上，进一步确定了由12个水稻SSR标记构成的核心标记组(每条染色体1个)，对127份安徽省审定(或主推)水稻品种进行荧光标记分析，构建供试水稻品种分子指纹，在此基础上，再与品种基本商品信息相结合，并进行数字化编码，构建了127份水稻的品种身份证。该身份证号码组成的第1部分为商品码，代表品种的基本商品信息，包括品种类别、品种选育(或审定)的区域和时间等；第2部分为指纹码，代表水稻品种的分子指纹信息；第3部分为补充码(或特异基因识别码)，代表品种的特异基因信息。还对127份水稻品种身份证进行了条码表述(一维码和二维码)，以便水稻品种种子的身份标识和溯源管理。

关键词: 水稻, SSR荧光标记, 毛细管电泳, 商品信息, 品种身份证

Abstract:

For increasing convenience of the rice seed quality tracking and management, we selected a set of 12 rice SSR markers (one marker for each chromosome) based on previous research. The fluorescent marker analysis was performed on 127 released and popularized authorized (or main plant) rice varieties in Anhui Province. Then, the rice variety ID was constructed by the SSR fingerprint with corresponded commodity information. The rice variety ID included three components: part 1 was recognized as commodity code, which reflected crop species, cultivar, breeding (or approval) region and year; part 2 was recognized as fingerprint code, which contained the variety DNA fingerprint information; part 3 was recognized as complement code (specific gene identification code), reflecting specific genetic information of cultivars. We also described the rice variety ID in the form of bar code or QR code, which might be favorable for rice seed identification and traceability management.

Key words: Rice, Fluorescent SSR Markers, Capillary Electrophoresis, Commodity Information, Variety ID

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