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作物学报 ›› 2015, Vol. 41 ›› Issue (01): 66-71.doi: 10.3724/SP.J.1006.2015.00066

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

津田芜菁泛素化相关蛋白基因BrRUB1的克隆及表达分析

闫海芳*,王斐,李桥,李鸿昌,杨虎城   

  1. 东北林业大学生命科学学院,黑龙江哈尔滨150040
  • 收稿日期:2014-06-20 修回日期:2014-09-30 出版日期:2015-01-12 网络出版日期:2014-11-11
  • 通讯作者: 闫海芳, E-mail: icyhf@yahoo.com
  • 基金资助:

    本研究由中央高校基本科研业务费专项(DL12CA10, DL11CA03, 2572014EA03-02)和国家基础科学人才培养基金(J1210053)资助。

Cloning and Expression Analysis of Ubiquitin-Related Protein Gene BrRUB1 in Brassica rapa ssp. rapifera Tsuda

YAN Hai-Fang*,WANG Fei,LI Qiao, I Hong-Chang,YANG Hu-Cheng   

  1. College of Life Science, Northeast Forestry University, Harbin 150040, China
  • Received:2014-06-20 Revised:2014-09-30 Published:2015-01-12 Published online:2014-11-11
  • Contact: 闫海芳, E-mail: icyhf@yahoo.com

摘要:

RUB1 [related to ubiquitin 1)是植物和酵母中一种泛素类似蛋白质,是Cullin家族的一个成员。为阐释津田芜菁BrRUB1基因的表达特性,本研究克隆了津田芜菁RUB1基因的全长cDNA序列,命名为BrRUB1GenBank登录号为KF501173。其ORF全长471 bp,编码156个氨基酸;亚细胞定位结果显示,BrRUB1-GFP定位于细胞核,表明BrRUB1蛋白可能在细胞核中发挥其功能。荧光定量PCR检测BrRUB1的表达表明,该基因表达量在花蕾中最高,花瓣中次之,具有组织特异性。而且Br RUB1在芜菁根皮中的表达受长波紫外线[UV-A)诱导。

关键词: 津田芜菁, RUB1, 基因克隆, 表达分析

Abstract:

RUB1 [related to ubiquitin 1) is a ubiquitin-like protein in plant and yeast, belong to Cullin family. To elucidate the expression feature of RUB1 in Tsuda, we isolated cDNA of RUB1 gene from this plant and named BrRUB1 [GenBank accession No. KF501173). cDNA of BrRUBI was 670 bp in full length and its open reading frame [ORF) was 471 bp in full length, encoding 157 amino acids. BrRUB1-GFP was localized to nucleus, indicating that BrRUB1 may play an important role in the nucleus. Quantitative-PCR analysis showed that BrRUB1 expressed highly in bud and less in petal, showing tissue specificity. Furthermore, the expression of BrRUB1 was induced by UV-A light in the swollen hypocotyls.

Key words: Brassica rapa Tsuda, RUB1, Gene cloning, Expression analysis

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