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作物学报 ›› 2016, Vol. 42 ›› Issue (04): 551-560.doi: 10.3724/SP.J.1006.2016.00551

• 耕作栽培·生理生化 • 上一篇    下一篇

水稻低叶绿素含量突变对光合作用及产量的影响

顾骏飞*,周振翔,李志康,戴琪星,孔祥胜,王志琴,杨建昌   

  1. 扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心,江苏扬州225009
  • 收稿日期:2015-08-19 修回日期:2016-01-11 出版日期:2016-04-12 网络出版日期:2016-01-25
  • 通讯作者: 顾骏飞, E-mail: gujf@yzu.edu.cn, Tel: 0514-87979381
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2015CB150401),国家自然科学基金项目(31501254,31371562),江苏省自然科学基金项目(BK20140480), 中国博士后基金(2014M550312, 2015T80590),江苏省高校自然科学基金项目(14KJB210007)和江苏省高校优势学科建设项目资助。

Effects of the Mutant with Low Chlorophyll Content onPhotosynthesis and Yield in Rice

GU Jun-Fei*,ZHOU Zhen-Xiang,LI Zhi-Kang,DAI Qi-Xing,KONG Xiang-Sheng,WANG Zhi-Qin,YANG Jian-Chang   

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
  • Received:2015-08-19 Revised:2016-01-11 Published:2016-04-12 Published online:2016-01-25
  • Contact: 顾骏飞, E-mail: gujf@yzu.edu.cn, Tel: 0514-87979381
  • Supported by:

    This study was supported by the National Basic Research Program of China (973, 2015CB150401), National Natural Science Foundation of China (31501254, 31371562), the Natural Science Foundation of Jiangsu Province (BK20140480),China Postdoctoral Science Foundation (2014M550312, 2015T80590), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (14KJB210007),and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要:

突变体水稻叶绿素含量仅是其野生型水稻的51%,但是其饱和光合值在低氮、中氮、高氮处理下,却比对照野生型水稻分别高3.7%、20.4%与39.1%。为了探究其生理学机制,分别在大田与盆栽试验中,不同氮肥水平研究了突变体材料与野生型材料的叶片Rubisco酶含量、气孔导度、水通道蛋白表达水平、叶绿素荧光、叶片解剖结构和叶绿体超微结构。叶绿体超微结构表明突变体材料虽然叶绿素含量降低,叶绿体的发育并未受到影响;叶绿素荧光试验结果表明,高光强下,低叶绿素含量突变体并未受到光抑制,光反应电子传递未受影响。气孔导度数据、叶片显微结构观察与水通道蛋白基因表达数据表明叶黄突变体具有较高的气孔与叶肉导度;同时低叶绿素含量突变体内较高的Rubisco酶含量也是其在高光照条件下具有较高光合速率的重要原因。产量数据表明,叶黄突变体虽然生育期短,但其产量水平与对照无显著差别,这可能与其高光强条件下有较高的光合速率有关。上述试验结果表明高叶绿素含量并不是叶片高光合速率的必需条件。在今后的高光效育种中,挑选叶绿素含量适宜的品种更有利于叶片内氮素在其他光合器官中的分配,提高光合效率,最终获得高光效品种。在本研究中使用的叶绿素含量降低突变体在高光效育种中有潜在的研究价值。

关键词: 水稻, 叶绿素, 光合作用, 高光效, 氮素

Abstract:

A chlorophyll-deficit rice mutant (YL) with ~51% chlorophyll of its wild type (WT) was 3.7%, 20.4%, and 39.1% higher in photosynthesisthan WT under saturating light condition,in treatments of0 kg N ha-1, 120 kg N ha-1, 240 kg N ha-1, respectively. In the field and pot experiments, we studied leaf Rubisco content, stomatal conductance, expression levels of aquaporin genes, chlorophyll fluorescence, light and electron micrographs at different levels of N application. The results showed that the decreased level of chlorophyll content in YL was compensated by a relativelyhigher quantum yield of PSII. The electron micrographs of chloroplasts showed that there were no differences in chloroplast development between YL and WT. The stomatal conductance was much higher in the mutant than in wild type, and expression levels of the aquaporin genes suggested a higher mesophyll conductance in YL. The higherCO2 conductance together with a higher Rubisco content in YL could be reasons for the higher photosynthetic rate. The yield of YL was similar to that of WT, but the growth duration in YL was much shorter, which could be caused by the different photosynthetic performance between YL and WT. All these results implicate that higher photosynthetic rate doesnot necessitate higher chlorophyll content. Moderate chlorophyll content will benefit the leaf photosynthesis. Decreasing N investment in chlorophyll synthesis and optimizing N distribution among different photosynthetic compounds could potentially improve photosynthesis and yield. The YL material used in this study could be potentially used to improve photosynthetic efficiency in breeding programmes.

Key words: Rice, Chlorophyll, Photosynthesis, High photosynthetic efficiency, Nitrogen

[1]Farquhar GD, von Caemmerer S, Berry JA. A biochemical model of photosynthetic CO2 assimilation in leaves of C3species. Planta, 1980, 149: 78–90

[2]von Caemmerer S, Farquhar G, Berry J. Biochemical model of C3 photosynthesis. In: Laisk A, Nedbal L, Govindjee G, eds. Photosynthesis in Silico: Understanding Complexity from Molecules to Ecosystems. Dordrecht, The Netherlands: Springer, 2009. pp209–230

[3]Gu J, Yin X, Stomph TJ, Wang H, Struik PC. Physiological basis of genetic variation in leaf photosynthesis among rice (Oryza sativa L.) introgression lines under drought and well-watered conditions. J Exp Bot, 2012, 63: 5137–5153

[4]Yin X, Struik PC, Romero P, Harbinson J, Evers JB, van der Putten PEL, Vos J. Using combined measurements of gas exchange and chlorophyll fluorescence to estimate parameters of a biochemical C3 photosynthesis model: a critical appraisal and a new integrated approach applied to leaves in a wheat (Triticumaestivum) canopy. Plant Cell Environ, 2009, 32: 448–464

[5]Long SP, Zhu X, Naidu SL, Ort DR. Can improvement in photosynthesis increase crop yields? Plant Cell Environ, 2006,29: 315–330

[6]Gu J, Yin X, Struik PC, Stomph TJ, Wang H. Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well watered field conditions. J Exp Bot, 2012,63: 455–469

[7]Zhu X, Long SP, Ort DR. What is the maximum ef?ciency with which photosynthesis can convert solar energy into biomass? CurrOpin Biotech, 2008, 19:153–159

[8]Murchie EH, Niyogi KK. Manipulation of photoprotection to improve plant photosynthesis. Plant Physiol, 2011, 155: 86–92

[9]Ort DR, Merchant SS, Alric J, Barkan A, Blankenship RE, Bock R, Croce R, Hanson MR, Hibberd JM, Long SP. Redesigning photosynthesis to sustainably meet global food and bioenergy demand. Proc Natl AcadSci USA, 2015,112: 8529–8536

[10]Ort DR, Zhu XG, Melis A. Optimizing antenna size to maximize photosynthetic efficiency. Plant Physiol, 2011, 155: 79–85

[11]Nakajima Y, Itayama T. Analysis of photosynthetic productivity of microalgal mass cultures. J ApplPhycol, 2003, 15: 497–505

[12]Kirst H, Formighieri C, Melis A. Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. BiochimBiophysActa, 2014, 1837:1653–1664

[13]Zhang H, Li J, Yoo JH, Yoo SC, Cho SH, Koh HJ, Seo SH, Paek N C. Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant MolBiol, 2006, 62: 325–337

[14]Jung KH, Hur J, Ryu CH, Choi Y, Chung YY, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463–472

[15]Wang PR, Gao JX, Wan CM, Zhang FT, Xu ZJ, Huang XQ, Sun XQ, Deng XJ. Divinyl chlorophyll (ide) a can be converted to monovinyl chlorophyll (ide) a by a divinyl reductase in rice. Plant Physiol, 2010, 153: 994–1003

[16]Sakuraba Y, Rahman ML, Cho SH, Kim YS, Koi HJ, Yoo SC, Paek NC. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J, 2013, 74: 122–133

[17]Wu ZM, Zhang X, He B, Diao LP, Sheng SL, Wang JL, Guo XP, Su N, Wang LF, Jiang L, Wang CM, Zhai HQ, Wan JM. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40

[18]Lee S, Kim JH, Yoo ES, Lee CH, Hirochika H, An G. Differential regulation of chlorophyll a oxygenase genes in rice. Plant MolBiol, 2005, 57: 805–818

[19]何旎清, 柳周, 张龙, 白苏阳, 田云录, 江玲, 万建民. 一个新的水稻黄绿叶突变体的遗传分析及突变基因的精细定位. 作物学报, 2015, 41: 1155–1163

He N Q, Liu Z, Zhang L, Bai S Y, Tian Y L, Jiang L, Wan J M. Genetic analysis of a new yellow-green mutant and fine-mapping of mutant gene in rice. ActaAgron Sin, 2015, 41: 1155–1163 (in Chinese with English abstract)

[20]郭涛, 黄永相, 罗文龙, 黄宣, 王慧, 陈志强, 刘永柱. 水稻叶色白化转绿及多分蘖矮秆突变体hfa-1的基因表达谱分析. 作物学报, 2013, 39: 2123–2134

Guo T, Huang Y X, Luo W L, Huang X, Wang H, Chen Z Q, Liu Y Z. Gene differential expression of a green-revertible albino and high-tillering dwarf mutant hfa-1 by using rice Microarray.ActaAgron Sin, 2013, 39:2123–2134 (in Chinese with English abstract)

[21]Holden M. Chlorophyll in Chemistry and Biochemistry of Plant Pigments,2nd edn. Goodwin TW,ed. Vol. 2, London: Academic Press, 1976.pp 1–37

[22]Makino A, Mae T, Chira K.Photosynthesis and rubulose-1,5-bisphosPhate carboxylase/oxygenase in rice leaves from emergence through senescence. Planta, 1985, 166: 414–420

[23]Makino A, MaeT, Chira K. Colorimetric measurement of protein stained with Coomassie Brilliant Blue Ron sodium dodecyl sulfate-polyacrylamide gel electrophoresis by eluting with formamide. AgricBiolChem, 1986, 50: 1911–1912

[24]Genty B, Briantais JM, Baker NR. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. BiochimBiophysActa, 1989,990: 87–92

[25]Maxwell K, Johnson GN. Chlorophyll fluorescence—a practical guide. J Exp Bot, 2000,51: 659–668

[26]Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol, 2005, 46: 1568–1577

[27]Yin X, Struik PC. Theoretical reconsiderations when estimating the mesophyll conductance to CO2 diffusion in leaves of C3 plants by analysis of combined gas exchange and chlorophyll fluorescence measurements. Plant Cell Environ, 2009, 32: 1513–1524 (corrigendum: Plant Cell Environ, 2009, 33: 1595)

[28]Yin X, Struik PC. Constraints to the potential efficiency of converting solar radiation into phytoenergy in annual crops: from leaf biochemistry to canopy physiology and crop ecology. J Exp Bot, 2015. DOI: 10.1093/jxb/erv371

[29]Zhu X, Sturler E D, Long SP. Optimizing the distribution of resources between enzymes of carbon metabolism can dramatically increase photosynthetic rate: a numerical simulation using an evolutionary algorithm. Plant Physiol, 2007, 145: 513–526

[30]Lefebvre S, Lawson T, Zakhleniuk OV, Lloyd JC, Raines CA. Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development. Plant Physiol, 2005, 138: 451–460

[31]Tamoi M, Nagaoka M, Miyagawa Y, Shigeoka S. Contribution of fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase to the photosynthetic rate and carbon ?ow in the Calvin cycle in transgenic plants. Plant Cell Physiol, 2006, 47: 380–390

[32]Peng S, Khush GS, Virk P, Tang Q, Zou Y. Progress in ideotype breeding to increase rice yield potential. Field Crops Res, 2008, 108: 32–38
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