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作物学报 ›› 2025, Vol. 51 ›› Issue (7): 1747-1756.doi: 10.3724/SP.J.1006.2025.44221

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆分枝数QTL定位及候选基因筛选

胡蒙,沙丹,张晟瑞,谷勇哲,张世碧,李静,孙君明*,邱丽娟*,李斌*   

  1. 作物基因资源与育种全国重点实验室 / 作物分子育种国家工程中心 / 农业农村部北京大豆生物学重点实验室 / 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2024-12-31 修回日期:2025-04-27 接受日期:2025-04-27 出版日期:2025-07-12 网络出版日期:2025-05-09
  • 基金资助:
    本研究由农业生物育种国家科技重大专项(2023ZD0403701)和中国农业科学院农业科技创新工程项目资助。

QTL mapping and candidate gene screening for branch number in soybean

HU Meng,SHA Dan,ZHANG Sheng-Rui,GU Yong-Zhe,ZHANG Shi-Bi,LI Jing,SUN Jun-Ming*,QIU Li-Juan*,LI Bin*   

  1. The State Key Laboratory of Crop Gene Resources and Breeding / National Engineering Research Center for Crop Molecular Breeding / Key Laboratory of Soybean Biology (Beijing), Ministry of Agriculture and Rural Affairs / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2024-12-31 Revised:2025-04-27 Accepted:2025-04-27 Published:2025-07-12 Published online:2025-05-09
  • Supported by:
    This study was supported by the Biological Breeding-National Science and Technology Major Project (2023ZD0403701), and the Agricultural Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.

摘要:

大豆是重要的粮饲兼用作物。分枝数是影响大豆产量的重要农艺性状之一。本研究以少分枝大豆品种中黄35与多分枝品种中黄13杂交衍生的RIL F2:7-8群体为材料,结合重测序基因型数据构建高密度遗传连锁图谱,采用QTL IciMapping完备复合区间定位方法,5个种植环境下定位分枝数相关的QTL结果显示,在2号、6号、18号和19号染色体上共定位6个与分枝数相关的QTL。其中位于2号染色体qVBN02-12环境中被检测到平均解释16.07%表型变异,为新发掘的分枝数稳定主效QTL。该位点区间遗传距离为0.3 cM,物理距离为261.37 kb,包含29个注释基因。经QTL区间内双亲错义SNP筛选,发掘出22个分枝数潜在候选基因。GO注释分析显示,这些基因编码的蛋白涉及多个影响植物生长发育的重要过程。本研究不仅为大豆株型改良提供了分子标记靶位点,也为精细定位大豆分枝数关键基因奠定了基础。

关键词: 大豆, 分枝数, 重组自交系, QTL定位, 候选基因

Abstract:

Soybean (Glycine max L.) is a vital crop widely used in both the food and feed industries. Branch number is a key agronomic trait that significantly influences soybean yield. In this study, we employed a recombinant inbred line (RIL) F2:7-8 population derived from a cross between the low-branching cultivar Zhonghuang 35 and the high-branching cultivar Zhonghuang 13. A high-density genetic linkage map constructed from resequencing-based genotypic data was used to identify quantitative trait loci (QTLs) associated with branch number across five different planting environments, using the inclusive composite interval mapping (ICIM) method implemented in QTL IciMapping software. A total of six QTLs related to branch number were detected on chromosomes 2, 6, 18, and 19. Among them, qVBN02-1, located on chromosome 2, was consistently identified in two environments and accounted for an average of 16.07% of the phenotypic variation, indicating that it is a novel, stable, and major QTL for branch number. This QTL spans a genetic interval of 0.3 cM, corresponding to a physical distance of 261.37 kb and encompassing 29 annotated genes. By analyzing missense single nucleotide polymorphisms (SNPs) between the two parental lines within this region, we identified 22 potential candidate genes. Gene Ontology (GO) annotation revealed that these genes are involved in various biological processes critical to plant growth and development. This study not only provides valuable molecular markers for improving soybean plant architecture but also lays a foundation for the fine mapping and functional characterization of genes regulating branch number in soybean.

Key words: soybean (Glycine max L.), branch number, recombinant inbred lines (RILs), QTL mapping, candidate genes

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