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作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1198-1214.doi: 10.3724/SP.J.1006.2025.41053
陆雯佳1,2(
), 汪军成1,2, 姚立蓉1,2, 张宏1,2, 司二静1,2, 杨轲1,2, 孟亚雄1,2, 李葆春1,3, 马小乐1,2, 王化俊1,2,*()
LU Wen-Jia1,2(), WANG Jun-Cheng1,2, YAO Li-Rong1,2, ZHANG Hong1,2, SI Er-Jing1,2, YANG Ke1,2, MENG Ya-Xiong1,2, LI Bao-Chun1,3, MA Xiao-Le1,2, WANG Hua-Jun1,2,*()
摘要：
过氧化物酶(Class III peroxidase, PRX)基因家族在调控植物生长发育及非生物胁迫过程中发挥重要作用。大麦(Hordeum vulgare L.)是典型的C3植物, 目前关于HvPRXs基因家族的功能研究鲜有报道。本研究通过生物信息学的方法对大麦PRX基因家族(HvPRXs)成员进行了分析, 探究其在20% PEG-6000胁迫下的表达模式。大麦全基因组中共鉴定出了178个HvPRXs基因家族成员, 并根据其在染色体上位置顺序依次命名为HvPRX1~HvPRX178。通过对大麦、水稻和拟南芥的过氧化物酶进行进化树分析将其分为5个亚家族, 基因结构和Domain分析表明同一亚家族具有较高的保守性。基因复制分析显示, 15个HvPRX基因(8%)存在片段复制, 34个HvPRX基因(67%)存在串联复制, 串联复制事件在HvPRX基因扩增中起重要作用。大麦与拟南芥的物种间共线性分析显示, 大麦与拟南芥有4个PRX直系同源基因对, 说明基因从单子叶到双子叶进行了大规模的分子进化事件。转录组分析显示, HvPRXs基因家族成员在大麦根、叶中表达存在差异。启动子顺式作用元件分析显示, 99个HvPRXs含有与干旱胁迫响应相关的顺式作用元件。最后基于qRT-PCR分析其干旱胁迫下的表达特征, 结果表明, HvPRX1、HvPRX18、HvPRX63、HvPRX160和HvPRX167受20% PEG-6000诱导在3 h表达量显著升高。本研究结果为全面探索HvPRXs基因的生物学功能、其调控大麦抗旱性的分子机制以及抗逆作物新品种的培育提供参考。
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