欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2025, Vol. 51 ›› Issue (9): 2454-2466.doi: 10.3724/SP.J.1006.2025.51016

• 耕作栽培·生理生化 • 上一篇    下一篇

小麦AL型细胞质雄性不育系与同型保持系穗花发育时期代谢物变化比较研究

孔德真(), 桑伟, 聂迎彬, 李伟, 徐红军, 李江博, 刘鹏鹏, 田笑明*()   

  1. 新疆农垦科学院作物研究所 / 谷物品质与遗传改良兵团重点实验室, 新疆石河子 832000
  • 收稿日期:2025-02-18 接受日期:2025-06-01 出版日期:2025-09-12 网络出版日期:2025-06-20
  • 通讯作者: *田笑明, E-mail: txming729@vip.sina.com
  • 作者简介:E-mail: kongdezhen1746@163.com
  • 基金资助:
    本研究由新疆生产建设兵团财政科技计划项目(2023CB007-10);本研究由新疆生产建设兵团财政科技计划项目(2024DA022)

Comparative analysis of metabolite changes during young panicle development in wheat AL type cytoplasmic male serile line and homologous maintainers

KONG De-Zhen(), SANG Wei, NIE Ying-Bin, LI Wei, XU Hong-Jun, LI Jiang-Bo, LIU Peng-Peng, TIAN Xiao-Ming*()   

  1. Institute of Crop Research, Xinjiang Academy of Agri-Reclamation Sciences / Key Lab of Xinjiang Production and Construction Corps for Cereal Quality Research and Genetic Improvement, Shihezi 832000, Xinjiang, China
  • Received:2025-02-18 Accepted:2025-06-01 Published:2025-09-12 Published online:2025-06-20
  • Contact: *E-mail: txming729@vip.sina.com
  • Supported by:
    Xinjiang Production and Construction Corps Financial Science and Technology Plan Project(2023CB007-10);Xinjiang Production and Construction Corps Financial Science and Technology Plan Project(2024DA022)

RichHTML

0

PDF

19

摘要：

研究小麦AL型细胞质雄性不育系与同型保持系穗花发育不同时期代谢组物质变化的关系, 为外源激素诱导细胞质雄性不育育性转换提供应用理论依据。本研究选用不育系AL-22A和同型保持系AL-22B为试验材料, 用国际法测定不育系套袋自交结实率。在穗发育二棱期、小花原基分化期、雌雄蕊原基分化期、药隔期、四分体期、单核中位期、单核靠边期、二核期、三核期等9个时期取穗样检测。采用液相色谱-质谱联用仪测定5种植物内源激素含量、过氧化物酶(POD)活性、活性氧(ROS)含量、ATP含量及非靶向代谢组学分析代谢物变化。不育系套袋自交结实率为0, 达到全不育水平; 在穗分化多个发育时期不育系与保持系比较, ABA含量高于保持系, IAA、ZR、GA3含量低于保持系, MeJA含量在穗分化二棱期至四分体期低于保持系, 单核期以后高于保持系; 在激素平衡关系中, IAA/ABA比值在不育系穗发育各期表现低于保持系, 不育系激素比值异常增高或降低均可能引发细胞质雄性不育, 其中ABA和MeJA含量增高对激素比值变化的影响最大; 生理代谢物ATP和ROS在不育系和保持系穗发育各期表现差异不显著, 在穗分化二棱期至四分体期保持系POD活性均显著高于不育系, 二核期和三核期不育系POD活性高于保持系; 非靶向代谢组研究发现, 雌雄蕊原基分化期和单核中位期不育系代谢物下调数目多于保持系, 其他时期不育系上调代谢物数目多于下调数目, 并且达到差异显著。对不育系和保持系穗发育各时期非靶向代谢物表达数目进行聚类分析, 可将其分为早期(I~III)、中期(IV~VI)、晚期(VII~IX) 3大类群, 穗发育不同时期代谢物数目的差异导致了小麦的育性变化; 代谢通路富集分析发现, 代谢物差异主要集中在小花分化期、药隔期、四分体期、单核靠边期、三核期, 代谢物主要以羧酸代谢、次级代谢为主。对小麦育性发育影响较大的时期为早中期(II~IV)和中后期(VI~VIII), 不同内源激素之间的平衡关系和代谢差异物数量异常可能是导致小麦细胞质雄性不育的主要原因。本研究为进一步揭示小麦细胞质雄性不育穗花发育的生理代谢机制以及探索AL型细胞质雄性不育系从不育转变为生理性可育提供了理论和应用依据。

关键词: 小麦, AL型, 细胞质雄性不育, 内源激素, 代谢组

Abstract:

To investigate the relationship between the AL-type cytoplasmic male sterile (CMS) wheat line and the metabolome of its maintainer line during spikelet development, and to provide a theoretical foundation for the use of exogenous hormones in regulating fertility restoration, the AL-22A sterile line and the AL-22B homotypic maintainer line were used as experimental materials. Self-seed setting rate was determined using international standard methods. Spike samples were collected at nine developmental stages: young panicle differentiation, double ridge, floret primordium differentiation, pistil and stamen differentiation, chamber formation, tetrad, mononuclear median, uninucleate, binucleate, and trinucleate stages. The levels of endogenous hormones, peroxidase (POD), reactive oxygen species (ROS), and ATP were measured using liquid chromatography-mass spectrometry (LC-MS), and metabolites were analyzed through non-targeted metabolomics. The self-seed setting rate of the sterile line was zero, indicating complete sterility. Compared to the maintainer line, the sterile line showed higher levels of abscisic acid (ABA) and lower levels of indole-3-acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid (GA3). Methyl jasmonate (MeJA) content was lower in the sterile line from the double ridge to tetrad stages but higher after the mononuclear median stage. The IAA/ABA ratio was consistently lower in the sterile line, suggesting that hormone imbalance may contribute to sterility, particularly the elevated levels of ABA and MeJA. No significant differences in ATP and ROS levels were observed between the two lines across developmental stages. However, POD activity was significantly higher in the maintainer line from the double ridge to tetrad stages, while the sterile line showed distinct POD levels at the binucleate and trinucleate stages. Non-targeted metabolomic analysis revealed that the sterile line had more downregulated metabolites during the pistil and stamen differentiation and mononuclear median stages, while other stages showed more upregulated than downregulated metabolites, with significant differences. Clustering analysis of metabolite expression patterns across developmental stages grouped samples into early (I-III), middle (IV-VI), and late (VII-IX) phases. Stage-specific differences in metabolite expression were associated with fertility changes. Pathway enrichment analysis indicated that differential metabolites were primarily concentrated in the floret primordium differentiation, chamber formation, tetrad, uninucleate, and trinucleate stages, mainly involving carboxylic acid metabolism and secondary metabolism. The stages most critical to fertility development occurred in early to mid (II-IV) and mid to late (VI-VIII) phases. These results suggest that the interplay between endogenous hormone balance and abnormal metabolite accumulation may be key factors in CMS. This study provides both theoretical insights and practical references for understanding the physiological and metabolic mechanisms of spikelet development in CMS wheat and for developing strategies to promote fertility restoration in AL-type sterile lines.

Key words: wheat, AL-type, CMS, endogenous hormones, metabolome

表1

不同发育时期具体取样日期"

系
Line		 发育时期Development stage (month/day) 自交结实率(国际法)
Self-seeds rate (international, %)
I II III IV V VI VII VIII IX
不育系 Sterile line (A) 04/09 04/19 04/24 04/28 05/03 05/06 05/09 05/11 05/13 0
保持系 Maintainer line (B) 04/09 04/19 04/24 04/28 05/03 05/06 05/09 05/11 05/13 100

附图1

5种激素外标法定量标准曲线"

图1

不育系和保持系不同时期内源激素含量变化 缩写同表1。A: 不育系; B: 保持系。ABA: 脱落酸; ZR: 玉米素核苷; MeJA: 茉莉酸甲酯; IAA: 生长素; GA3: 赤霉素。不同小写字母表示差异达5%显著水平。"

表2

不育系和保持系内源激素含量之间比值的关系"

项目Item 材料Material I II III IV V VI VII VIII IX
IAA/ABA A 0.50 1.17 0.40 0.35 0.26 0.29 2.69 0.69 2.89
B 0.97 2.21 0.49 0.94 0.66 0.69 3.87 3.53 4.13
B-A 0.47 1.04 0.09 0.59 0.40 0.40 1.18 2.84 1.24
IAA/GA3 A 5.09 41.69 16.79 44.18 29.20 21.30 43.46 1.25 4.57
B 6.71 24.16 71.28 29.73 24.89 1.99 3.81 5.86 129.94
B-A 1.62 -17.53 54.49 -14.45 -4.31 -19.31 -39.65 4.61 125.37
ABA/GA3 A 10.12 35.65 42.27 125.45 111.59 73.46 16.17 1.81 1.58
B 6.94 10.92 145.97 31.51 37.96 2.87 0.98 1.66 31.43
B-A -3.18 -24.73 103.70 -93.94 -73.63 -70.59 -15.19 -0.15 29.85
MeJA/IAA A 17.19 3.03 3.83 1.00 1.46 71.66 40.08 18.87 0.98
B 21.98 21.39 8.39 5.33 9.95 4.05 3.82 2.00 1.76
B-A 4.79 18.36 4.56 4.33 8.49 -67.61 -36.26 -16.87 0.78
ZR/ IAA A 28.89 14.62 18.57 9.60 12.86 45.00 15.03 24.42 8.93
B 16.50 17.33 13.54 11.16 8.02 20.95 12.84 8.38 7.34
B-A -12.39 2.71 -5.03 1.56 -4.84 -24.05 -2.19 -16.04 -1.59
ZR/ GA3 A 147.13 609.41 311.74 423.92 375.51 958.56 653.39 30.51 40.84
B 110.67 418.64 965.37 331.77 199.58 41.58 48.91 49.09 953.25
B-A -36.46 -190.77 653.63 -92.15 -175.93 -916.98 -604.48 18.58 912.41
MeJA/ GA3 A 87.57 126.52 64.36 44.29 42.65 1526.45 1742.01 23.58 4.50
B 147.38 516.89 598.18 158.33 247.66 8.04 14.54 11.72 228.15
B-A 59.81 390.37 533.82 114.04 205.01 -1518.41 -1727.47 -11.86 223.65

图2

不育系和保持系在小麦发育不同时期生理代谢物变化 缩写同表1。A: 不育系; B: 保持系。不同小写字母表示差异达5%显著水平。"

图3

不育系和保持系在小麦发育不同时期代谢物差异分析 缩写同表1。"

图4

不育系和保持系在小穗发育各时期前10类富集的差异代谢物"

图5

不育系和保持系不同时期差异代谢物的KEGG通路分析 缩写同表1。"

[1] 孔德真, 聂迎彬, 徐红军, 穆培源, 崔凤娟, 桑伟, 田笑明. AL型三系交小麦不育相关基因dCAPs标记开发与应用. 分子植物育种, 2023, 21: 3336-3340.
Kong D Z, Nie Y B, Xu H J, Mu P Y, Cui F J, Sang W, Tian X M. Development and application of dCAPs markers related to sterility genes in AL type three-line hybrid wheat. Mol Plant Breed, 2023, 21: 3336-3340 (in Chinese with English abstract).
[2] Zhang S W, Zhang D, Fan S, Du L S, Shen Y W, Xing L B, Li Y M, Ma J J, Han M Y. Effect of exogenous GA3 and its inhibitor paclobutrazol on floral formation, endogenous hormones, and flowering-associated genes in ‘Fuji’ apple (Malus domestica Borkh.). Plant Physiol Biochem, 2016, 107: 178-186.
[3] 李罗江, 茹振刚, 高庆荣, 姜辉, 郭凤芝, 吴世文, 孙哲. BNS小麦的雄性不育性及其温光特性. 中国农业科学, 2009, 42: 3019-3027.
doi: 10.3864/j.issn.0578-1752.2009.09.002
Li L J, Ru Z G, Gao Q R, Jiang H, Guo F Z, Wu S W, Sun Z. Male sterility and thermo-optical characteristics of BNS wheat. Sci Agric Sin, 2009, 42: 3019-3027 (in Chinese with English abstract).
[4] 周美兰, 宋雪薇, 朱潇, 姜辉, 郭凤芝, 吴世文, 孙哲. 小麦温敏不育系BNS366育性转换的敏感期研究. 麦类作物学报, 2015, 35: 7-15.
Zhou M L, Song X W, Zhu X, Jiang H, Guo F Z, Wu S W, Sun Z. Study on sensitive period of fertility transition of wheat temperature-sensitive sterile line BNS366. J Triticeae Crops, 2015, 35: 7-15 (in Chinese with English abstract).
[5] 刘海英, 冯必得, 茹振钢, 陈向东, 黄培新, 邢晨涛, 潘茵茵, 甄俊琦. BNS和BNS366小麦雄性不育与内源激素的关系. 中国农业科学, 2021, 54(1): 1-18.
doi: 10.3864/j.issn.0578-1752.2021.01.001
Liu H Y, Feng B D, Ru Z G, Chen X D, Huang P X, Xing C T, Pan Y Y, Zhen J Q. Relationship between BNS and BNS366 wheat male sterility and endogenous hormones. Sci Agric Sin, 2021, 54: 1-18 (in Chinese with English abstract).
[6] 李英贤, 张爱民, 梁振兴. 小麦雄性不育的发生与花药组织内激素平衡的关系. 农业生物技术学报, 1998, 6(1): 71-75.
Li Y X, Zhang A M, Liang Z X. Relationship between BNS and BNS366 wheat male sterility and endogenous hormones. J Agric Biotechnol, 1998, 6(1): 71-75 (in Chinese with English abstract).
[7] 张自刚, 马小飞, 张红霞, 王震, 张思妮, 郭冬, 张永鹏, 马翎健. 小麦光温敏雄性不育系BNS育性转换与内源激素的关系研究. 植物遗传资源学报, 2016, 17: 913-919.
doi: 10.13430/j.cnki.jpgr.2016.05.018
Zhang Z G, Ma X F, Zhang H X, Wang Z, Zhang S N, Guo D, Zhang Y P, Ma L J. Relationship between fertility transition and endogenous hormones in wheat temperature-sensitive male sterile line BNS. J Plant Genet Resour, 2016, 17: 913-919 (in Chinese with English abstract).
[8] 张龙雨, 袁蕾, 杨书玲, 张改生, 王俊生, 宋瑜龙, 赵卓军, 牛娜, 马守才. 小麦雄性不育系中TaPDC-E1α及其调节酶基因的表达特征. 作物学报, 2011, 37: 620-628.
doi: 10.3724/SP.J.1006.2011.00620
Zhang L Y, Yuan L, Yang S L, Zhang G S, Wang J S, Song Y L, Zhao Z J, Niu N, Ma S C. Expression characteristics of TaPDC-E1α and its regulatory enzyme gene in male sterile wheat lines. Acta Agron Sin, 2011, 37: 620-628 (in Chinese with English abstract).
[9] 苗锦山, 杨文才, 刘彩霞, 李美芹, 乔宁, 沈火林. 葱胞质雄性不育花蕾生化物质含量和能量代谢酶活性的动态变化特征. 西北植物学报, 2010, 30: 1142-1148.
Miao J S, Yang W C, Liu C X, Li M Q, Qiao N, Shen H L. Dynamic changes of biochemical substances content and energy metabolizing enzyme activity in cytoplasmic male sterile flower buds of Allium allium L. Acta Bot Boreali-Occident Sin, 2010, 30: 1142-1148 (in Chinese with English abstract).
[10] 王保明, 陈永忠, 李红波, 莫华, 黄露波. 植物雄性不育的机制及应用研究进展. 河南农业科学, 2019, 48(5): 1-9.
Wang B M, Chen Y Z, Li H B, Mo H, Huang L B. Research progress on mechanism and application of male sterility in plants. J Henan Agric Sci, 2019, 48(5): 1-9 (in Chinese with English abstract).
[11] 张鹏飞, 宋瑜龙, 张改生, 赵新亮, 巴青松, 刘红占, 祝万万, 李志宽, 王军卫, 牛娜. 小麦雄性不育系绒毡层异常代谢与小孢子败育的关系. 中国农业科学, 2014, 47: 1670-1680.
doi: 10.3864/j.issn.0578-1752.2014.09.002
Zhang P F, Song Y L, Zhang G S, Zhao X L, Ba Q S, Liu H Z, Zhu W W, Li Z K, Wang J W, Niu N. Relationship between abnormal tapetum metabolism and microspore abortion in male sterile lines of wheat. Sci Agric Sin, 2014, 47: 1670-1680 (in Chinese with English abstract).
[12] Li Y, Liu T K, Duan W K, Song X M, Shi G J, Zhang J Y, Deng X H, Zhang S N, Hou X L. Instability in mitochondrial membranes in Polima cytoplasmic male sterility of Brassica rapa ssp. chinensis. Funct Integr Genomics, 2014, 14: 441-451.
[13] 贲蓓倍, 徐维红, 邹德玉, 牟美睿, 杨仁杰, 刘海学. 不同施肥条件下的小麦籽粒代谢组学研究. 麦类作物学报, 2021, 41: 212-219.
Ben B B, Xu W H, Zou D Y, Mu M R, Yang R J, Liu H X. Study on metabolomics of wheat grain under different fertilization conditions. J Triticeae Crops, 2021, 41: 212-219 (in Chinese with English abstract).
[14] 于振文. 作物栽培学各论(北方本). 北京: 中国农业出版社, 2003.
Yu Z W. Crop Cultivation Monographs (Northern Edition). Beijing: China Agricultural Press, 2003 (in Chinese).
[15] 宋喜悦, 何蓓如, 马翎健, 胡银岗, 李宏斌. 小麦温敏不育系A3314温敏不育性的遗传研究. 中国农业科学, 2005, 38: 1095-1099.
Song X Y, He B R, Ma L J, Hu Y G, Li H B. Genetic study on temperature-sensitive sterility of wheat temperature-sensitive sterile line A3314. Sci Agric Sin, 2005, 38: 1095-1099 (in Chinese with English abstract).
[16] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
Li H S. Experimental Principles and Techniques of Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000 (in Chinese).
[17] 王贺正, 张均, 吴金芝, 徐国伟, 陈明灿, 付国占, 李友军. 不同氮素水平对小麦旗叶生理特性和产量的影响. 草业学报, 2013, 22(4): 69-75.
doi: 10.11686/cyxb20130408
Wang H Z, Zhang J, Wu J Z, Xu G W, Chen M C, Fu G Z, Li Y J. Effects of different nitrogen levels on physiological characteristics and yield of flag leaves of wheat. Acta Pratac Sin, 2013, 22(4): 69-75 (in Chinese with English abstract).
[18] 柏斌, 吴俊, 盛文涛, 庄文, 李莺歌, 邓启云. 育性敏感期低温胁迫对水稻光温敏不育系叶片内源激素的影响. 杂交水稻, 2016, 31(1): 57-61.
Bai B, Wu J, Sheng W T, Zhuang W, Li Y G, Deng Q Y. Effects of different nitrogen levels on physiological characteristics and yield of flag leaves of wheat. Hybrid Rice, 2016, 31(1): 57-61 (in Chinese with English abstract).
[19] 张艳玉, 张卫东, 高庆荣, 张保雷, 李楠楠, 高建华, 王慧娜, 赵兰飞. 温光敏雄性不育小麦BNS幼穗发育中的内源激素变化. 西北植物学报, 2013, 33: 1165-1170.
Zhang Y Y, Zhang W D, Gao Q R, Zhang B L. Li N N, Gao J H, Wang H N, Zhao L F. Endogenous hormone changes in young ear development of temperature-photosensitive male sterile wheat BNS. Acta Bot Boreali-Occident Sin, 2013, 33: 1165-1170 (in Chinese with English abstract).
[20] 陈建国, 王建波, 胡如忠, 陈钊德, 杨振智, 丁丁, 郭大芳, 谢晋云, 安开宇, 等. 烟草胞质雄性不育系与保持系内源植物激素水平差异性研究. 安徽农业科学, 2018, 46(9): 54-57.
Chen J G, Wang J B, Hu R Z, Chen Z D, Yang Z Z, Ding D, Guo D F, Xie J Y, An K Y, et al. Study on the difference of endogenous plant hormone levels between cytoplasmic male sterile line and maintainer line of tobacco. J Anhui Agric Sci, 2018, 46(9): 54-57 (in Chinese with English abstract).
[21] 杨海燕. 温光敏核不育小麦育性转换与激素间关系的研究. 西南大学硕士学位论文, 重庆, 2006.
Yang H Y. Study on the Relationship between Fertility Conversion and Hormones in Temperature-photosensitive Sterile Wheat. MS Thesis of Southwest University, Chongqing, China, 2006 (in Chinese with English abstract).
[22] 贺晓敏, 周美兰, 余传启, 蒋敏明, 茹振刚. 温敏雄性不育小麦BNS366花粉败育的细胞学观察. 麦类作物学报, 2014, 34: 460-466.
He X M, Zhou M L, Yu C Q, Jiang M M, Ru Z G. Cytological observation of pollen abortion in Wenmin male sterile wheat BNS366. J Triticeae Crops, 2014, 34: 460-466 (in Chinese with English abstract).
[23] 刘海英, 甄俊琦, 胡铁柱, 茹振钢, 李珍, 胡雪寒, 邢晨涛, 高远. 小麦温敏雄性不育系BNS366小孢子发育和花粉育性检测方法研究. 麦类作物学报, 2018, 38: 379-385.
Liu H Y, Zhen J Q, Hu T Z, Ru Z G, Li Z, Hu X H, Xing S T, Gao Y. Study on detection methods of microspore development and pollen fertility of wheat temperature-sensitive male sterile line BNS366. J Triticeae Crops, 2018, 38: 379-385 (in Chinese with English abstract).
[24] 丛庆, 张琪, 宋丽莉, 陈月, 蔡洪生, 郭长虹. 激素在植物冷胁迫应答中的角色. 核农学报, 2016, 30: 614-619.
doi: 10.11869/j.issn.100-8551.2016.03.0614
Cong Q, Zhang Q, Song L L, Chen Y, Cai H S, Guo C H. The role of hormones in plant response to cold stress. J Nucl Agric Sci, 2016, 30: 614-619 (in Chinese with English abstract).
[25] 孙佳尧, 李资文, 崔天宇, 刘鹏, 赵丽梅, 李志刚. 大豆不育系花器官发育过程中内源激素动态变化研究. 大豆科学, 2020, 39: 891-897.
Sun J Y, Li Z W, Cui T Y, Liu P, Zhao L M, Li Z G. Dynamic changes of endogenous hormones during flower organ development in sterile soybean lines. Soybean Sci, 2020, 39: 891-897 (in Chinese with English abstract).
[26] 邓继新, 刘文芳, 肖翊华. HPGMR花粉发育期花药ATP含量及核酸与蛋白质的合成研究. 武汉大学学报(自然科学版), 1990, 36(3): 85-88.
Deng J X, Liu W F, Xiao Y H. Study on anther ATP content and synthesis of nucleic acid and protein in HPGMR pollen during its development. J Wuhan Univ (Nat Sci Edn), 1990, 36(3): 85-88 (in Chinese with English abstract).
[27] 周凯, 司龙亭, 张琪. 萝卜细胞质雄性不育系与保持系光合特性和呼吸特性的比较试验. 北方园艺, 2007, (6): 3-5.
Zhou K, Si L T, Zhang Q. Comparative study on photosynthetic and respiratory characteristics of cytoplasmic male sterile line and maintainer line of turnip. North Hortic, 2007, (6): 3-5 (in Chinese with English abstract).
[28] 冯小磊, 范光宇, 苏旭, 宋国亮, 史高雷, 王峰, 邱风仓, 王晓明, 赵治海. 植物雄性不育生理生化研究进展. 作物杂志, 2012, (3): 6-11.
Feng X L, Fan G Y, Su X, Song G L, Shi G L, Wang F, Qiu F C, Wang X M, Zhao Z H. Advances in physiological and biochemical studies of male sterility in plants. Crops, 2012, (3): 6-11 (in Chinese with English abstract).
[29] 刘金兵, 侯喜林, 陈晓峰, 张静宜, 王述彬, 潘宝贵. 甜椒胞质雄性不育系及其保持系生化特性研究. 园艺学报, 2006, 33: 629-631.
Liu J B, Hou X L, Chen X F, Zhang J Y, Wang S B, Pan B G. Study on biochemical characteristics of cytoplasmic male sterile line and its maintainer line of sweet pepper. Acta Hortic Sin, 2006, 33: 629-631 (in Chinese with English abstract).
[30] Wang S P, Zhang Y X, Song Q L, Fang Z W, Chen Z, Zhang Y M, Zhang L L, Zhang L, Niu N, Ma S C, et al. Mitochondrial dysfunction causes oxidative stress and tapetal apoptosis in chemical hybridization reagent-induced male sterility in wheat. Front Plant Sci, 2018, 8: 2217.
[31] 刘玉平, 茹振刚, 陈希勇, 李辉, 李亚军, 赵爱菊, 宿振起. 小麦不育系BNS在石家庄地区育性转换规律的研究. 河北农业科学, 2011, 15(6): 46-49.
Liu Y P, Ru Z G, Chen X Y, Li H, Li Y J, Zhao A J, Su Z Q. Study on fertility conversion of wheat sterile line BNS in Shijiazhuang area. J Hebei Agric Sci, 2011, 15(6): 46-49 (in Chinese with English abstract).
[1] 李云香, 郭千纤, 侯万伟, 张小娟. 引进ICARDA小麦苗期根系抗旱性状的全基因组关联分析[J]. 作物学报, 2025, 51(9): 2387-2398.
[2] 蒋环琪, 段奥, 郭超, 黄晓梦, 艾德骏, 刘小雪, 谭静怡, 彭成林, 李曼菲, 杜何为. 渍水胁迫对玉米幼苗根系代谢的影响[J]. 作物学报, 2025, 51(9): 2295-2306.
[3] 胡润慧, 汪军成, 司二静, 张宏, 李兴茂, 马小乐, 孟亚雄, 王化俊, 刘青, 姚立蓉, 李葆春. 小麦苗期耐旱耐盐种质筛选及抗旱耐盐综合评价[J]. 作物学报, 2025, 51(9): 2371-2386.
[4] 杨颖聪, 张俊豪, 唐一哲, 乔唱唱, 王鹏博, 黄明, 徐国伟, 王贺正. 秸秆还田和施磷量对旱地小麦籽粒淀粉及其合成相关酶活性的影响[J]. 作物学报, 2025, 51(9): 2467-2484.
[5] 李璐琪, 程宇坤, 白斌, 雷斌, 耿洪伟. 小麦叶片气孔相关性状全基因组关联分析[J]. 作物学报, 2025, 51(9): 2266-2284.
[6] 杨婷婷, 陈娟, ABDUL Rehman, 李婧, 闫素辉, 汪建来, 李文阳. 花后弱光对软质小麦干物质积累转运、籽粒产量和淀粉品质的影响[J]. 作物学报, 2025, 51(8): 2204-2219.
[7] 张飞飞, 何万龙, 焦文娟, 白斌, 耿洪伟, 程宇坤. 小麦抗条锈病相关性状元分析及候选基因分析[J]. 作物学报, 2025, 51(8): 2111-2127.
[8] 闫喆林, 任强, 樊志龙, 殷文, 孙亚丽, 范虹, 何蔚, 胡发龙, 闫丽娟, 柴强. 氮肥后移优化绿洲灌区小麦间作玉米种间关系提高氮素利用效率[J]. 作物学报, 2025, 51(8): 2190-2203.
[9] 宋改利, 王璐倩, 屈柯飞, 唐建卫, 董纯豪, 黄振朴, 高艳, 牛吉山, 殷贵鸿, 李巧云. Bipolaris sorokiniana黑胚病对中筋小麦淀粉含量、粒度分布与糊化特性的影响[J]. 作物学报, 2025, 51(8): 2164-2175.
[10] 王曜阔, 王文政, 张敏, 刘希伟, 杨敏, 李昊昱, 张灵鑫, 闫彦菲, 蔡瑞国. 水氮运筹对冬小麦籽粒GMP合成和面粉加工品质的影响[J]. 作物学报, 2025, 51(8): 2176-2189.
[11] 高梦娟, 赵贺莹, 陈家辉, 陈晓倩, 牛萌康, 钱琪润, 崔陆飞, 邢江敏, 银庆淼, 郭雯, 张宁, 孙丛苇, 阳霞, 裴丹, 贾奥琳, 陈锋, 余晓东, 任妍. 小麦抗纹枯病新位点Qse.hnau-5AS的定位及其候选基因鉴定[J]. 作物学报, 2025, 51(8): 2240-2250.
[12] 姜朋, 吴磊, 黄倩楠, 李畅, 王化敦, 何漪, 张鹏, 张旭. 矮秆基因Rht-D1在长江中下游麦区的育种利用探索[J]. 作物学报, 2025, 51(8): 2077-2086.
[13] 鲁向前, 付玉洁, 赵俊恒, 郑楠楠, 孙楠楠, 张国平, 叶玲珍. 小麦花药培养最佳取样时期穗部形态特征鉴定与高培养力基因型筛选[J]. 作物学报, 2025, 51(8): 2033-2047.
[14] 蔡金珊, 李超男, 王景一, 李宁, 柳玉平, 景蕊莲, 李龙, 孙黛珍. 小麦幼苗根系性状全基因组关联分析及TaSRL-3B优异等位基因发掘[J]. 作物学报, 2025, 51(8): 2020-2032.
[15] 吴柳格, 陈坚, 张鑫, 邓艾兴, 宋振伟, 郑成岩, 张卫建. 近二十年国审冬小麦品种的产量与品质性状变化趋势研究[J]. 作物学报, 2025, 51(7): 1814-1826.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2