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作物学报 ›› 2012, Vol. 38 ›› Issue (04): 571-577.doi: 10.3724/SP.J.1006.2012.00571

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

硬粒小麦品种Waskana和Waskowa对禾谷孢囊线虫(Heterodera filipjevi和H. avenae)的抗性

高秀1,2，崔磊2,3，李洪连3，王晓鸣2，唐文华4，Robert L. CONNER5，林小虎1,*，李洪杰2,*   

  1. 1 河北科技师范学院生命科技学院，河北秦皇岛 066004；2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程，北京100081；3 河南农业大学植物保护学院，河南郑州450002；4 中国农业大学植物病理系，北京100193；5 Agriculture and Agri-Food Canada, Morden Research Station, Unit 100-101 Route 100, Morden, MB R6M 1Y5 Canada
  • 收稿日期:2011-11-03 修回日期:2012-01-19 出版日期:2012-04-12 网络出版日期:2012-02-14
  • 通讯作者: 李洪杰, E-mail: hongjie@caas.net.cn; 林小虎, E-mail: xiaohulin2008@163.com
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(200903040), 国家重点基础研究计划(973计划)项目(2009CB118300), 科技部中澳特别基金项目(2010DFA31380), 国家现代农业产业体系建设项目(CARS-3-1)和国家自然科学基金项目(31171545)资助。

Resistance of Triticum durum Cultivars Waskana and Waskowa to Cereal Cyst Nematode, Heterodera filipjevi and H. avenae

GAO Xiu1,2,CUI Lei2,3,LI Hong-Lian3,WANG Xiao-Ming2,TANG Wen-Hua4,Robert L. CONNER5,LIN Xiao-Hu1,*,LI Hong-Jie2,*   

  1. 1 Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China; 4 Department of Plant Pathology, China Agricultural University, Beijing 100193, China; 5 Agriculture and Agri-Food Canada, Morden Research Station, Unit 100-101 Route 100, Morden, MB R6M 1Y5 Canada
  • Received:2011-11-03 Revised:2012-01-19 Published:2012-04-12 Published online:2012-02-14
  • Contact: 李洪杰, E-mail: hongjie@caas.net.cn; 林小虎, E-mail: xiaohulin2008@163.com

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摘要： 禾谷孢囊线虫(cereal cyst nematode, CCN)是一类重要的土传小麦病原线虫，危害我国小麦的主要是燕麦孢囊线虫(Heterodera avenae)和菲利普孢囊线虫(H. filipjevi)。我国对这些病原线虫的抗性资源十分缺乏，寻找新抗源是当前抗性育种的重要工作。本研究通过3年的田间病圃和温室接种鉴定，发现加拿大的硬粒小麦品种Waskana和Waskowa对H. filipjevi (河南许昌群体，Hfc-1致病型)和H. avenae (河南荥阳群体，Ha43致病型)都表现很强的抗性，单株孢囊数显著少于感病的普通小麦品种矮抗58、石4185和温麦19。显微观察可见，虽然两种线虫的幼虫都能够侵入Waskana和Waskowa的根组织内，但是根内的线虫数量显著少于感病对照普通小麦品种，最终在根系上形成的可见孢囊数量也较少。Waskana和Waskowa对两种病原线虫的抗性为我国抗CCN小麦品种选育提供了有较高利用价值的新抗源。根据南澳大利亚研究所的土传病害检测服务系统对土壤中病原线虫的分子检测结果，抗CCN品种Waskana和Waskowa根际土壤中的线虫虫卵量低于感病小麦品种，因此种植可能降低土壤中禾谷孢囊线虫危害的风险。

关键词: 硬粒小麦, 禾谷孢囊线虫, Heterodera filipjevi, H. avenae, 抗病性

Abstract: Cereal cyst nematodes (CCN) are a group of important soil-borne pathogens of wheat. Heterodera avenae and H. filipjevi are the major species of CCN that have limited wheat (Triticum aestivum) production in China in recent years. Since the CCN-resistant resource is short, it is important to develop new sources with resistance for breeding purpose in China. Based on the results of a 3-year field test and controlled environment tests, we found that two Canadian Triticum durum cultivars, Waskana and Waskowa, were highly resistant to both Heterodera filipjevi (pathotype Hfc-1) and H. avenae (pathotype HA43) populations from Henan Province, China. The number of white females per plant in Waskana and Waskowa was significantly smaller than that in the susceptible common wheat cultivars Aikang 58, Shi 4185, and Wenmai 19. Although the juveniles of H. filipjevi and H. avenae populations were able to penetrate into the roots of Waskana and Waskowa, the number of juveniles inside the roots was significantly smaller than that of Aikang 58, Shi 4185, and Wenmai 19, resulting in smaller numbers of females on the roots of these durum wheat cultivars. Waskana and Waskowa with resistance to H. filipjevi and H. avenae are useful in developing wheat cultivars with enhanced resistance to CCN in China. Based on the results from the DNA-based soil testing service operated by South Australian Research and Development Institute, the number of eggs of nematodes in the rhizospheric soil samples from the CCN-resistant cultivars Waskana and Waskowa was less than that in the soil samples from the susceptible wheat cultivars. This indicates that application of resistant cultivars might reduce the risk of damage caused by CCN in soil.

Key words: Triticum durum, Cereal cyst nematode (CCN), Heterodera filipjevi, H. avenae, Resistance

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