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作物学报 ›› 2013, Vol. 39 ›› Issue (11): 1944-1951.doi: 10.3724/SP.J.1006.2013.01944
王瑞1,2,**,吴华玲3,**,王会芳1,2,黄珂1,2,霍春艳1,2,倪中福1,2,孙其信1,2,*
WANG Rui1,2,**,WU Hua-Ling3,**,WANG Hui-Fang1,2,HUANG Ke1,2,HUO Chun-Yan1,2,NI Zhong-Fu1,2,SUN Qi-Xin1,2,*
摘要：
WRKY是植物特有的转录因子基因, 在植物对外界胁迫响应及生长发育的过程中发挥重要作用。本研究克隆了一个新的小麦WRKY转录因子基因TaWRKY44, 获得其全长cDNA, 其中开放阅读框长度为897 bp, 编码298个氨基酸。半定量RT-PCR的结果表明, TaWRKY44在叶片中表达水平较高, 并且受干旱和低温胁迫诱导表达。转基因功能分析结果表明, TaWRKY44的拟南芥超表达株系叶片变小, 叶柄缩短, 并且叶片细胞也明显小于野生型。另外, 转基因系对ABA、干旱和盐等胁迫处理的敏感性也高于野生型, 说明该基因可能作为一个转录抑制子参与逆境胁迫信号转导过程。
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