作物学报 ›› 2016, Vol. 42 ›› Issue (07): 966-975.doi: 10.3724/SP.J.1006.2016.00966
郭丹,施勇烽,王惠梅,张晓波,宋莉欣,徐霞,贺彦,郭梁,吴建利*
GUO Dan,SHI Yong-Feng,WANG Hui-Mei,ZHANG Xiao-Bo,SONG Li-Xin,XU Xia,HE Yan,GUO Liang,WU Jian-Li*
摘要:
通过EMS (ethane methyl sulfonate)诱变籼稻品种IR64获得一个稳定遗传的显性斑点叶突变体HM113。在大田环境下,突变体褐色斑点在播种后3周的叶片上产生,始穗期扩散至叶鞘。与野生型IR64相比,突变体HM113的株高、结实率和千粒重等农艺性状显著下降,光合色素含量、净光合速率和可溶性蛋白含量显著降低。同时突变体CAT和SOD活性显著降低,POD活性显著上升。组织化学分析显示,突变体叶片中积累了大量活性氧,且斑点处细胞坏死。白叶枯病菌接种结果显示,HM113是一个广谱抗性增强的突变体。实时定量PCR分析表明HM113中防卫反应基因AOS2、PAL4、PR10和PR1b等的表达大幅上调。遗传分析表明,突变体褐斑性状受单显性基因(SplHM113)控制,利用图位克隆法将该基因定位在第7染色体长臂RM21605和RM418之间,物理距离约为308 kb。本研究为褐斑基因SplHM113的克隆与功能分析奠定了基础。
[1]Heath M C. Hypersensitive response-related death. Plant Mol Biol, 2000, 44: 321–334
[2]夏启中, 吴家和, 张献龙. 与植物超敏反应(HR)相关的细胞编程性死亡. 华中农业大学学报, 2005, 24: 97–103 Xia Q Z, Wu J H, Zhang X L. Review on hypersensitive response-related PCD in plant. J Huazhong Agric Univ, 2005, 24: 97–103 (in Chinese with English abstract) [3]Durrant W E, Dong X. Systemic acquired resistance. Annu Rev Phytopathol, 2004, 42: 185–209 [4]Hu G, Yalpani N, Briggs S P, Johal G S. A porphyrin pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize. Plant Cell, 1998, 10: 1095–1105 [5]Balague C, Lin B, Alcon C, Flottes G, Malmstrom S, Kohler C, Neuhaus G, Pelletier G, Gaymard F, Roby D. HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family. Plant Cell, 2003, 15: 365–379 [6]Feng B H, Yang Y, Shi Y F, Shen H C, Wang H M, Huang Q N, Xu X, Lv X G, Wu J L. Characterization and genetic analysis of a novel rice spotted-leaf mutant HM47 with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J Integr Plant Biol, 2013, 55: 473–483 [7]Shen H C, Shi Y F, Feng B H, Wang H M, Xu X, Huang Q N, Lv X G, Wu J L. Identification and genetic analysis of a novel rice spotted-leaf mutant with broad-spectrum resistance to Xanthomonas oryzae pv. oryzae. J Integr Agric, 2014, 13: 713–721 [8]李小红, 施勇烽, 张晓波, 奉保华, 宋莉欣, 王惠梅, 徐霞, 黄奇娜, 郭丹, 吴建利. 水稻斑点叶突变体hm197的鉴定及其基因定位. 中国水稻科学, 2015, 29: 447–456 Li X H, Shi Y F, Zhang X B, Feng B H, Song L X, Wang H M, Xu X, Huang Q N, Guo D, Wu J L. Identification and gene mapping of a spotted leaf mutant hm197 in rice. Chin J Rice Sci, 2015, 29: 447–456 (in Chinese with English abstract) [9]Jung Y H, Lee J H, Agrawal G K, Rakwal R, Kim J A, Shim J K, Lee S K, Jeon J S, Koh H J, Lee Y H, Iwahashi H, Jwa N S. The rice (Oryza sativa) blast lesion mimic mutant, blm, may confer resistance to blast pathogens by triggering multiple defense-associated signaling pathways. Plant Physiol Biochem, 2005, 43: 397–406 [10]Wu C, Bordeos A, Madamba M R, Baraoidan M, Ramos M, Wang G L, Leach J E, Leung H. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet Genomics, 2008, 279: 605–619 [11]Fujiwara T, Maisonneuve S, Isshiki M, Mizutani M, Chen L, Wong H L, Kawasaki T, Shimamoto K. Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice. J Biol Chem, 2010, 285: 11308–11313 [12]Wang L, Pei Z, Tian Y, He C. OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol Plant Microbe Interact, 2005, 18: 375–384 [13]Sun C, Liu L, Tang J, Lin A, Zhang F, Fang J, Zhang G, Chu C. RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice. J Genet Genomics, 2011, 38: 29–37 [14]Sakuraba Y, Rahman M L, Cho S H, Kim Y S, Koh H J, Yoo S C, Paek N C. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J, 2013, 74: 122–133 [15]Yamanouchi U, Yano M, Lin H, Ashikari M, Yamada K. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci USA, 2002, 99: 7530–7535 [16]Jiang C J, Shimono M, Maeda S, Inoue H, Mori M, Hasegawa M, Sugano S, Takatsuji H. Suppression of the rice fatty-acid desaturase gene OsSSI2 enhances resistance to blast and leaf blight diseases in rice. Mol Plant Microbe Interact, 2009, 22: 820–829 [17]Qiao Y, Jiang W, Lee J, Park B, Choi M S, Piao R, Woo M O, Roh J H, Han L, Paek N C, Seo H S, Koh H J. SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit micro 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). New Phytol, 2010, 185: 258–274 [18]Fekih R, Tamiru M, Kanzaki H, Abe A, Yoshida K, Kanzaki E, Saitoh H, Takagi H, Natsume S, Undan J R, Undan J, Terauchi R. The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response. Mol Genet Genomics, 2015, 290: 611–622 [19]Arnon D I. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol, 1949, 24: 1–15 [20]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectro-photometers of different resolution. Plant Physiol, 2015, 144: 307–313 [21]赵世杰, 史国安, 董新纯. 植物生理学实验指导. 北京: 中国农业科学技术出版社, 2002. pp 134–143 Zhao S J, Shi G A, Dong X C. Plant Physiology Experiment Instruction. Beijing: China Agricultural Science and Technology Press, 2002. pp 134–143 (in Chinese) [22]Huang Q N, Shi Y F, Zhang X B, Song L X, Feng B H, Wang H M, Xu X, Li X H, Guo D, Wu J L. Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. J Integr Plant Biol, 2016, 58: 12–28 [23]Yin Z, Chen J, Zeng L, Goh M, Leung H, Khush G S, Wang G L. Characterizing rice lesion mimic mutants and identifying a mutant with broad-spectrum resistance to rice blast and bacterial blight. Mol Plant Microbe Interact, 2000, 13: 869–876 [24]Kauffman H E, Reddy A P D, Ksiek S P V, Marca S D. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 1973, 57: 537–541 [25]卢扬江, 郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学, 1992, 6: 47–48 Lu Y J, Zheng K L. A simple method for isolation of rice DNA. Chin J Rice Sci, 1992, 6: 47–48 (in Chinese with English abstract) [26]Shi Y F, Chen J, Liu W Q, Huang Q N, Shen B, Leung H, Wu J L. Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.). Sci China Ser C-Life Sci, 2009, 52: 885–890 [27]代高猛, 朱小燕, 李云峰, 凌英华, 赵芳明, 杨正林, 何光华. 水稻类病斑突变体spl31的遗传分析与基因定位. 作物学报, 2013, 39: 1223–1230 Dai G M, Zhu X Y, Li Y F, Ling Y H, Zhao F M, Yang Z L, He G H. Genetic analysis and fine mapping of a lesion mimic mutant spl31 in rice. Acta Agron Sin, 2013, 39: 1223–1230 (in Chinese with English abstract) [28]宋莉欣, 黄奇娜, 奉保华, 施勇烽, 张晓波, 徐霞, 王惠梅, 李小红, 赵宝华, 吴建利. 水稻斑点叶突变体spl21的鉴定与基因定位. 作物学报, 2015, 41: 1519–1528 Song L X, Huang Q N, Feng B H, Shi Y F, Zhang X B, Xu X, Wang H M, Li X H, Zhao B H, Wu J L. Characterization and gene mapping of a spotted-leaf mutant spl21 in rice (Oryza sativa L.). Acta Agron Sin, 2015, 41: 1519–1528 (in Chinese with English abstract) [29]Xiao G Q, Zhang H W, Lu X Y, Huang R F. Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.). J Integr Agric, 2015, 14: 1687–1696 [30]Jacks Thomas J, Davidonis Gayle H. Superoxide, hydrogen peroxide, and the respiratory burst of fungally infected plant cells. Mol Cellular Biochem, 1996, 158: 77–79 [31]Asada K. Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant, 1992, 85: 235–241 [32]Minibayeva F, Beckett R P, Kranner I. Roles of apoplastic peroxidases in plant response to wounding. Phytochemistry, 2015, 112: 122–129 [33]Kawano T. Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep, 2003, 21: 829–837 [34]Whitaker C, Beckett R P, Minibayevab F V, Kranner I. Production of reactive oxygen species in excised, desiccated and cryopreserved explants of Trichilia dregeana Sond. S Afr J Bot, 2010, 76: 112–118 [35]金杨. 水稻类病变突变体spl5细胞坏死机制及其抗病性的研究. 浙江师范大学硕士学位论文, 浙江金华, 2009 Jin Y. Mechanisms of Cell Death and Its Resistance in Rice Lesion Mimic Mutant spl5. MS Theises of Zhejiang Normal University, Jinhua, China, 2009 (in Chinese with English abstract) [36]Kaurilind E, Xu E, Brosche M. A genetic framework for H2O2 induced cell death in Arabidopsis thaliana. BMC Genomics, 2015, 16: 837–853 [37]Kariola T, Brader G, Li J, Palva E T. Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell, 2005, 17: 282–294 [38]Chern M, Fitzgerald H A, Canlas P E, Navarre D A, Ronald P C. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol Plant Microbe Interact, 2005, 18: 511–520 [39]Mori M, Tomita C, Sugimoto K, Hasegawa M, Hayashi N, Dubouzet J G, Ochiai H, Sekimoto H, Hirochika H, Kikuchi S. Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice. Plant Mol Biol, 2007, 63: 847–860 [40]钟振泉, 罗文龙, 刘永柱, 王慧, 陈志强, 郭涛. 一份新的水稻斑点叶突变体spl32的鉴定和基因定位. 作物学报, 2015, 41: 861–871 Zhong Z Q, Luo W L, Liu Y Z, Wang H, Chen Z Q, Guo T. Characterization of a novel spotted leaf mutant spl32 and mapping of Spl32(t) gene in rice (Oryza sativa). Acta Agron Sin, 2015, 41: 861–871 (in Chinese with English abstract) [41]Hwang S H, Hwang D J. Isolation and characterization of the rice NPR1 promoter. Plant Biotechnol Rep, 2010, 4: 29–35 [42]Yuan Y X, Zhong S H, Li Q, Zhu Z R, Lou Y G, Wang L Y, Wang J J, Wang M Y, Li Q L, Yang D L, He Z H. Functional analysis of rice NPR1-like genes reveals that OsNPR1/NHI is the rice orthologue conferring disease resistance with enhanced herbivore susceptibility. Plant Biotechnol J, 2007, 5: 313–324 [43]Li Z, Zhang Y X, Liu L, Liu Q E, Bi Z Z, Yu N, Cheng S H, Cao L Y. Fine mapping of the lesion mimic and early senescence 1 (lmes1) in rice (Oryza sativa). Plant Physiol Biochem, 2014, 80: 300–307 [44]Chen X F, Pan J W, Cheng J, Jiang G H, Jin Y, Gu Z M, Qian Q, Zhai W X, Ma B J. Fine genetic mapping and physical delimitation of the lesion mimic gene spotted leaf 5 (spl5) in rice (Oryza sativa L.). Mol Breed, 2009, 24: 387–395 [45]Babu R, Jiang C J, Xu X, Kottapalli K R, Takatsuji H, Miyao A, Hirochika H, Kawasaki S. Isolation, fine mapping and expression profiling of a lesion mimic genotype, spl(NF4050-8) that confers blast resistance in rice. Theor Appl Genet, 2011, 122: 831–854 |
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