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作物学报 ›› 2018, Vol. 44 ›› Issue (01): 95-104.doi: 10.3724/SP.J.1006.2018.00095

• 耕作栽培·生理生化 • 上一篇    下一篇

北方白菜型冬油菜的膜脂脂肪酸组分和ATPase活性对温度的响应

方彦1,2,孙万仓1,2,*,武军艳2,刘自刚2,董云2,3,米超2,马骊2,陈奇2,何辉立2   

  1. 1 甘肃省干旱生境作物学重点实验室,甘肃兰州 730070;2 甘肃省油菜工程技术研究中心, 甘肃兰州 730070;3 甘肃省农业科学院作物研究所, 甘肃兰州 730070
  • 收稿日期:2017-01-20 修回日期:2017-09-10 出版日期:2018-01-12 网络出版日期:2017-09-28
  • 通讯作者: 孙万仓, E-mail: 18293121851@163.com, Tel: 18293121851
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-12),国家自然科学基金项目(31460356, 31560397),国家重点基础研究发展计划项目(973计划) (2015CB150206)和甘肃农业大学盛彤笙创新基金(GSAU-STS-1428)资助。

Response of Membrane Fatty Acid Composition and ATPase Activity in Brassica rapa L. to Temperature in North China

FANG Yan1,2,SUN Wan-Cang1,2,*,WU Jun-Yan2,LIU Zi-Gang2,DONG Yun2,3,MI Chao2,MA Li2,CHEN Qi2,HE Hui-Li2   

  1. 1 Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Lanzhou 730070, Gansu, China; 2 Rape Engineering and Technology Research Center of Gansu Provincial, Lanzhou 730070, Gansu, China; 3 Crops Institute of Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2017-01-20 Revised:2017-09-10 Published:2018-01-12 Published online:2017-09-28
  • Contact: Sun Wancang, E-mail: 18293121851@163.com, Tel: 18293121851
  • Supported by:

    This study was supported by the National Modern Agro-industry Technology System (CARS-13), the National Natural Science Foundation of China (31460356, 31560397), the National Key Basic Research Program of China (973 Program) (2015CB150206), and the Sheng Tongsheng Innovation Fund of Gansu Agricultural University (GSAU-STS-1428).

摘要:

为了解北方白菜型冬油菜膜脂脂肪酸和ATPase活性与抗寒性的关系,以抗寒性强的冬油菜品种陇油7号和抗寒性弱的品种天油2号为材料,研究了不同温度处理(25°C、10°C、2°C、–5°C)后叶片和根系膜脂脂肪酸组分和ATPase酶活性的变化。结果表明,低温胁迫下2个冬油菜品种叶片和根系膜脂脂肪酸组分相同,叶片中不饱和脂肪酸以亚麻酸为主,根系不饱和脂肪酸以亚油酸为主。随处理温度的降低,2个冬油菜品种叶片不饱和脂肪酸含量呈先降低(10°C,2°C)后增加(–5°C)的趋势;陇油7号根中不饱和脂肪酸含量逐步增加,天油2号则逐步降低;在低温条件下(2°C,–5°C),陇油7号膜脂U/S比值、IUFA值高于天油2号;ATPase活性表现为陇油7号逐渐高于天油2号。说明2个冬油菜品种的膜脂在低温响应上存在一定差异,低温下不饱和脂肪酸含量和ATPase活性的提高是强抗寒冬油菜品种在北方旱寒区严酷环境条件下能安全越冬的重要原因。

关键词: 冬油菜, 脂肪酸, ATP酶活性, 抗寒性

Abstract:

Brassica rapa L. cultivars Longyou 7 (cold tolerant) and Tianyou 2 (cold sensitive) were used to investigate the variations of membrane fatty acid composition and ATPase activity at the temperatures of 25°C, 10°C, 2°C, and –5°C. There was the same membrane fatty acid composition in leaf and root of two cultivars under temperature stresses, with linolenic acid as the main component of unsaturated fatty acid in leaf, but linoleic acid in root. With decrease of treatment temperatures, the content of unsaturated fatty acid in leaf initially decreased at 10°Cand 2°C, then increased at –5°C.The content of unsaturated fatty acid rose up gradually in root of Longyou 7, but reduced in Tianyou 2. At low temperature(2°C, –5°C), the ratio of U/S and IUFA in Longyou 7 were significantly higher than those in Tianyou 2.The activity of ATPase was gradually increased in Longyou 7 than in Tianyou 2. It suggested there are differences in membrane lipids in response to temperature for two winter rapeseed cultivars, the increase of unsaturated fatty acid content and ATPase activity is the main cause sustaining winter rapeseed cultivars to overwinter.

Key words: winter rapeseed, fatty acid, ATPase activity, cold tolerance

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