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作物学报 ›› 2021, Vol. 47 ›› Issue (2): 275-284.doi: 10.3724/SP.J.1006.2021.04105

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

河南大豆新品系抗大豆疫霉根腐病基因鉴定

张雪翠1(), 孙素丽1(), 卢为国2, 李海朝2, 贾岩岩1, 段灿星1, 朱振东1,*()   

  1. 1中国农业科学院作物科学研究所, 北京 100081
    2河南省农业科学院经济作物研究所, 河南郑州 450002
  • 收稿日期:2020-05-12 接受日期:2020-09-13 出版日期:2021-02-12 网络出版日期:2020-09-28
  • 通讯作者: 朱振东
  • 作者简介:张雪翠, E-mail: 553870151@qq.com;|孙素丽, E-mail: sunsuli@caas.cn
  • 基金资助:
    “十三五”国家重点研发计划项目(2016YFD0100201);国家公益性行业(农业)科研专项(201303018);农作物种质资源保护与利用专项(2019NWB036-12);中国农业科学院科技创新工程项目资助

Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province

ZHANG Xue-Cui1(), SUN Su-Li1(), LU Wei-Guo2, LI Hai-Chao2, JIA Yan-Yan1, DUAN Can-Xing1, ZHU Zhen-Dong1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2Institute of Economic Crops, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
  • Received:2020-05-12 Accepted:2020-09-13 Published:2021-02-12 Published online:2020-09-28
  • Contact: ZHU Zhen-Dong
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100201);Special Fund for Agro-scientific Research in the Public Interest(201303018);Program of Protection of Crop Germplasm Resources(2019NWB036-12);Scientific Innovation Program of Chinese Academy of Agricultural Sciences.

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摘要：

大豆疫霉根腐病作为影响大豆生产的毁灭性病害之一, 对大豆生产威胁很大。种植抗疫霉根腐病的大豆品种是控制该病害最有效的途径。河南省位于我国黄淮夏大豆产区的腹地, 具有大豆疫霉根腐病发生的潜在威胁。本研究的目的是对河南省新育成的大豆品系进行抗性鉴定和抗病基因分子标记检测, 以明确大豆新品系对大豆疫霉根腐病的抗性水平和抗病基因。采用下胚轴创伤接种法对64个河南省培育的大豆新品系进行接种, 鉴定其对2个具有不同毒力的大豆疫霉分离物PsJS2和Ps41-1的抗性。结果显示, 对分离物Ps41-1和PsJS2抗病的分别有35个和16个品系, 对Ps41-1和PsJS2为中间反应型的分别有16个和10个品系, 其中对2个分离物均抗病的有16个品系, 占鉴定品系的25%。使用抗疫霉病基因RpsZheng共分离标记WZInDel11进行新品系的基因型鉴定发现, 对2个大豆疫霉分离物均抗病的16个品系中有13个含有标记WZInDel11, 对1个或2个大豆疫霉分离物表现为中间反应型的5个大豆品系, 分子检测结果表明, 其为杂合基因型, 这些品系中的纯合抗病单株可直接选育成纯合抗病品系用于抗病育种。综合系谱分析结果推测, 有2个品系可能含抗疫霉根腐病基因RpsZheng, 2个品系可能含RpsYD29, 14个品系可能含有RpsZheng或其等位基因。表明河南省培育的大豆新品系中含有优异的大豆疫霉根腐病抗源, 该研究结果将为病害防控和抗病品种的选育提供参考。

关键词: 大豆, 大豆疫霉根腐病, 品系, 抗病性, 分子标记

Abstract:

Phytophthora root rot is one of the destructive diseases affecting soybean production, which is a great threat to soybean production. Planting resistant soybean cultivars is the most effective way to control this disease. Henan province was located in the hinterland area of the summer-sowing soybean production region of Huang-Huai in China, which had the potential threat region of phytophthora root rot. The objective of this study was to screen effective resistance cultivars for disease control and resistance breeding by phenotypic identification and molecular detection of resistance gene. Sixty-four new soybean lines bred in Henan were evaluated for their resistance responses to two Phytophthora sojae isolates PsJS2 and Ps41-1 using the hypocotyls inoculation technique. The result showed that 35 lines and 16 lines were resistance to Ps41-1 and PsJS2, respectively. Sixteen lines and 10 lines were intermediate to Ps41-1 and PsJS2, respectively. And there were 16 lines resistance to both Ps41-1and PsJS2, accounting for 25% of tested lines. Sixty-four lines was detected for Phytophthora resistance gene by using molecular marker WZInDel11 co-segregating with a resistance gene RpsZheng. The results showed that, 13 of 16 lines resistant to both PsJS2 and Ps41-1 contain target band of WZInDel11, while 5 lines resistant to one of two P. sojae isolates show segregating to P. sojae produced heterozygous bands. The homozygous resistant plants of these lines segregating for resistance could be accurately detected by marker WZInDel11, and further were directly developed into homozygous resistant lines. Combining the results of pedigree analysis, it was speculated that two lines might contain the resistance gene RpsZheng, two lines might contain RpsYD29, and 14 lines might contain RpsZheng or its allele. In conclusion, the results indicated that the new soybean lines cultivated in Henan Province had excellent resistance sources to P. sojae. This study provides important information for disease control and resistance breeding.

Key words: Glycine max, phytophthora root rot, lines, resistance, molecular marker

表1

大豆新品系对2个大豆疫霉分离物Ps41-1和PsJS2的抗性及分子标记WZInDel11的鉴定结果"

图1

大豆品系对大豆疫霉分离物Ps41-1和PsJS2的抗性反应型的比例 R: 抗病; I: 中间型; S: 感病。"

图2

标记WZInDel11在部分大豆品系上的分子检测结果 Williams和郑97196分别为感病和抗病品种对照。洛豆16106、郑豆1304、洛豆16095、周豆47和郑豆1526为5个抗病品系。周豆39、洛豆1420、商豆191、濮豆103和宛黄5号为5个感病品系。郑15234、漯8825、漯豆8816、驻豆37和泛豆22为5个存在抗性分离的品系。"

图3

标记WZInDel11在大豆品系漯豆8816的10个单株上的分子检测结果 Williams和郑97196分别为感病和抗病品种对照, 漯豆8816-S为6个感病单株, 漯豆8816-R为4个抗病单株。"

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