小麦品系CH7034中耐盐QTL定位

doi:10.3724/SP.J.1006.2022.11074

作物学报 ›› 2022, Vol. 48 ›› Issue (10): 2654-2662.doi: 10.3724/SP.J.1006.2022.11074

小麦品系CH7034中耐盐QTL定位

张潇文¹(), 李世姣¹, 张晓军², 李欣², 杨足君³, 张树伟², 陈芳², 常利芳², 郭慧娟², 畅志坚²^,^*(), 乔麟轶²^,^*()

¹山西大学生命科学学院, 山西太原 030006
²山西农业大学农学院 / 作物遗传与分子改良山西省重点实验室 / 省部共建有机旱作农业国家重点实验室(筹), 山西太原 030031
³电子科技大学生命科学与技术学院, 四川成都 610054

收稿日期:2021-08-22 接受日期:2022-01-06 出版日期:2022-10-12 网络出版日期:2022-02-24
通讯作者: 畅志坚,乔麟轶
作者简介:第一作者联系方式: E-mail: zxw18435139058@126.com
基金资助:
山西省回国留学人员科研资助项目(2021-070);山西农业大学博士科研启动项目(2021BQ39);山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202002-3)

QTL mapping for salt tolerance in wheat line CH7034

ZHANG Xiao-Wen¹(), LI Shi-Jiao¹, ZHANG Xiao-Jun², LI Xin², YANG Zu-Jun³, ZHANG Shu-Wei², CHEN Fang², CHANG Li-Fang², GUO Hui-Juan², CHANG Zhi-Jian²^,^*(), QIAO Lin-Yi²^,^*()

¹College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
²College of Agriculture, Shanxi Agricultural University / Shanxi Key Laboratory of Crop Genetics and Molecular Improvement / State Key Laboratory of Sustainable Dryland Agriculture (in preparation), Taiyuan 030031, Shanxi, China
³College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

Received:2021-08-22 Accepted:2022-01-06 Published:2022-10-12 Published online:2022-02-24
Contact: CHANG Zhi-Jian,QIAO Lin-Yi
Supported by:
Shanxi Scholarship Council of China(2021-070);Shanxi Agricultural University Research Project for Doctor(2021BQ39);State Key Laboratory of Sustainable Dryland Agriculture (in preparation) of Shanxi Agricultural University(202002-3)

摘要/Abstract

摘要：

鉴定小麦耐盐种质对于充分利用盐碱地和保障粮食安全具有重要意义。CH7034是本实验室自育的1份小麦耐盐品系, 为了明确其耐盐性遗传规律和控制位点, 利用CH7034与盐敏感品种SY95-71的重组自交系群体进行QTL分析。基于SNP芯片数据和盐害指数(salt injury index), 在2A、2D、4B和5A染色体上共检测出6个QTL, 分别为QSI.sxau_2A、QSI.sxau_2D、QSI.sxau_4B.1、QSI.sxau_4B.2、QSI.sxau_5A.1和QSI.sxau_5A.2。其中, QSI.sxau_5A.1在3次盐胁迫试验中均能被检测到, 具有最高的表型变异解释率(15.73%~20.18%), 且不同于5AL染色体上已报道的其他耐盐位点。在QSI.sxau_5A.1区间开发并整合了7个SSR标记, 将LOD峰值进一步确定在SSR-D1处。基于转录组数据库, 从QSI.sxau_5A.1区段内筛选了12个响应盐胁迫的高置信基因。研究结果为CH7034耐盐位点的精细定位乃至克隆奠定了基础, 也为小麦耐盐品种选育提供了新种质和分子标记。

关键词: 小麦, 盐胁迫, 盐害指数, SNP标记, QTL定位

Abstract:

The identification of wheat salt-tolerant germplasm is of great significance for making full use of saline land and ensuring food security. CH7034 is a wheat salt-tolerant line bred by our laboratory. In order to clarify the genetic rules and control loci for salt tolerance, QTL analysis was conducted using a recombinant inbred lines population crossed by CH7034 and salt-sensitive variety SY95-71. Based on the SNP microarray data and salt injury index, six QTL were detected on chromosomes 2A, 2D, 4B, and 5A, including QSI.sxau_2A, QSI.sxau_2D, QSI.sxau_4B.1, QSI.sxau_4B.2, QSI.sxau_5A.1, and QSI.sxau_5A.2. Among them, QSI.sxau_5A.1 was detected in all three salt stress experiments and had the highest phenotypic variance explained (15.73%-20.18%), which was different from other salt-tolerance loci reported on chromosome 5AL. Seven SSR markers were developed and integrated in the QSI.sxau_5A.1 interval, and the peak of LOD was further determined at SSR-D1. Based on the transcriptome database, 12 high-confidence genes in response to salt stress were screened from the QSI.sxau_5A.1 section. These results lay the foundation for the fine mapping and even cloning of the salt tolerance loci of CH7034, and provide new germplasm and molecular markers for the selection and breeding of wheat salt-tolerant varieties.

Key words: wheat, salt stress, salt injury index, SNP marker, QTL mapping

张潇文, 李世姣, 张晓军, 李欣, 杨足君, 张树伟, 陈芳, 常利芳, 郭慧娟, 畅志坚, 乔麟轶. 小麦品系CH7034中耐盐QTL定位[J]. 作物学报, 2022, 48(10): 2654-2662.

ZHANG Xiao-Wen, LI Shi-Jiao, ZHANG Xiao-Jun, LI Xin, YANG Zu-Jun, ZHANG Shu-Wei, CHEN Fang, CHANG Li-Fang, GUO Hui-Juan, CHANG Zhi-Jian, QIAO Lin-Yi. QTL mapping for salt tolerance in wheat line CH7034[J]. Acta Agronomica Sinica, 2022, 48(10): 2654-2662.

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标记名称 Marker name	上游引物 Forward sequence (5'-3')	下游引物 Reverse sequence (5'-3')	产物长度 Product size (bp)	退火温度 Annealing temperature (℃)
SSR-U7	GGTCATGTTGGAATGTTGATCAAG	GCTTTTATGTTTCCAACTCTAGTCAA	127	58
SSR-D28	GGGAAATCCCAACGCCTG	GAGTCATCATGAAAGAAAGCATAAAT	144	58
SSR-D5	GCCCACGTTTCCTCTTAATG	TCATCGAACATGATGCACTATC	94	58
SSR-D1	GAGAATAAATCCACTCTCCATGC	CGATATGTTCCTGTCTCCTTGT	180	58
SSR-D4	CCGTGTAGTAGAACACTGCATGT	GCTCGAGCTGTAGCCCAG	193	54
SSR-U2	AAGCAGAATCATGGAAGGGAC	CACTTATTCAAGTCATTAAGCATTCAAT	102	58
SSR-U5	CAGTATCCATCTTTGCCATTCAG	CTCCTACCTCGACGACAC	116	58
cslinkNax2	TCTCCATCATTCAACATCAATCG	TGTAGCTCGTCGGGGTGTGTTGC	170	58
Xgwm304	AGGAAACAGAAATATCGCGG	AGGACTGTGGGGAATGAATG	176	58
Xbarc144	GCGTTTTAGGTGGACGACATAGATAGA	GCGCCACGGGCATTTCTCATAC	191	58
Xwmc110	GCAGATGAGTTGAGTTGGATTG	GTACTTGGAAACTGTGTTTGGG	127	58

材料 Material	总株数 Total number of plants	盐害级别及株数 Salt injury level and number of plants					盐害指数 Salt injury index (%)
材料 Material	总株数 Total number of plants	0	1	2	3	4	盐害指数 Salt injury index (%)
CH7034-1	16		7	8	1		32.5
CH7034-2	16		8	6	2		32.5
CH7034-3	16		8	8			30.0
平均值 Mean							31.7
SY97-71-1	16				2	14	77.5
SY97-71-2	16				3	13	76.3
SY97-71-3	16				2	14	77.5
平均值 Mean							77.1

试验编号 Test number	数量性状位点 QTL	染色体 Chr.	图谱位置 Position (cM)	标记区间 Marker region	LOD值 LOD score	表型变异解释率 PVE (%)	加性效应 Add.
SI-1	QSI.sxau_2D	2D	0	992489-992489	3.57	5.60	-2.42
	QSI.sxau_4B.1	4B	112	1265612-3954524	6.38	10.50	-3.31
	QSI.sxau_5A.1	5A	73	1049519-4911066	6.63	15.73	-3.81
SI-2	QSI.sxau_2A	2A	9	3532866-1090021	2.96	5.24	-2.71
	QSI.sxau_4B.2	4B	43	1862515-1059340	3.41	6.36	-2.96
	QSI.sxau_5A.1	5A	71	1049519-1215538	10.29	20.18	-5.49
SI-3	QSI.sxau_5A.1	5A	71	1049519-1215538	10.15	17.62	-4.50
	QSI.sxau_5A.2	5A	116	1125437-1228444	4.27	15.73	-3.09

基因/QTL Gene/QTL	参考文献 Reference	耐盐表型 Phenotype	来源 Source	连锁标记 Linkage marker
基因/QTL Gene/QTL	参考文献 Reference	耐盐表型 Phenotype	来源 Source	标记名称 Name	亲本多态性 Polymorphism in parents	与表型连锁Linked to phenotype
TmHKT1;5-A	[6,7]	叶片K⁺/Na⁺ K⁺/Na⁺ ratio of leaf	Triticum durum	cslinkNax2	+	-
QTL-SR3	[9]	叶片盐害级别 Salt injury level of leaf	wheat	Xgwm304	-	-
QTL-SR3	[10]	叶片盐害级别 Salt injury level of leaf	wheat	Xbarc144	+	-
qNL5, qLL5	[8]	叶数, 叶长 Leaf number, leaf length	Triticum durum	Xwmc110	-	-
QHAI.5A-2	[11]	盐碱地收获指数 Harvest index in salt land	wheat	wPt-3334
QG(1-5).asl-5A	[12]	相对生长率 Relative growth rate (RGR) salt/RGR control	wheat	TP14539

差异表达基因 DEGs	基因组位置 Genomic location	差异倍数 log₂ (Fold Change)	响应类别 Response type	编码蛋白注释 Protein-encoding annotation
TraesCS5A01G382800	580799964-580801653	1.71	上调 Upregulate	钙调素相关钙感应蛋白 Calmodulin-related calcium sensor protein
TraesCS5A01G382900	580803022-580805966	-1.44	下调 Downregulate	ATP硫酸化酶 ATP sulfurylase
TraesCS5A01G386500	584049719-584058870	-1.08	下调 Downregulate	肌球蛋白 Myosin protein
TraesCS5A01G388300	585018687-585021441	1.26	上调 Upregulate	β-葡萄糖苷酶前体 β-glucosidase precursor
TraesCS5A01G389000	585232865-585236702	1.26	上调 Upregulate	β-葡萄糖苷酶前体 β-glucosidase precursor
TraesCS5A01G390200	585488957-585491921	1.01	上调 Upregulate	胡豆苷合酶 Strictosidine synthase
TraesCS5A01G392100	588416260-588416877	4.68	上调 Upregulate	无 None
TraesCS5A01G393900	589311576-589313132	2.25	上调 Upregulate	UDP-葡萄糖基转移酶 UDP-glucosyl transferase
TraesCS5A01G394500	589584058-589585518	1.38	上调 Upregulate	UDP-葡萄糖基转移酶 UDP-glucosyl transferase
TraesCS5A01G394900	590540043-590541485	1.38	上调 Upregulate	UDP-葡萄糖6-磷酸脱氢酶 UDP-glucose 6-phosphatedehydrogenase
TraesCS5A01G397600	591976101-591977663	-1.85	下调 Downregulate	脂肪酶3类家族蛋白 Lipase class 3 family protein
TraesCS5A01G402600	595371952-595372493	-1.01	下调 Downregulate	细胞色素6f复合亚基 Cytochrome b6f complex subunit

小麦品系CH7034中耐盐QTL定位

QTL mapping for salt tolerance in wheat line CH7034

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