Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 580-587.doi: 10.3724/SP.J.1006.2009.00580

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Quantitative Trait Loci Analysis of Responses to Nitrogen and Phosphorus Deficiency in Rice Chromosomal Segment Substitution Lines

WANG Yu,SUN Yong-Jian,CHEN Deng-Ying,YU Si-Bin*   

  1. National Key Laboratory of Crop Genetic Improvement and National Plant Gene Research Centre (Wuhan),Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-08-11 Revised:2008-12-13 Online:2009-04-12 Published:2009-02-13
  • Contact: YU Si-Bin,E-mail:ysb@mail.hzau.edu.cn;Tel:027-87281803 E-mail:ysb@mail.hzau.edu.cn

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Abstract:

Tolerance to low nitrogen and low phosphorus conditions is a highly desired characteristic for sustainable crop production. In this study, a set of 125 chromosome segment substitution lines (CSSL), each containing a single or few introgression segments from a japonica cv. Nipponbare with the genetic background of an indica cv. 9311, were evaluated using augmented design under the field experiment with normal, low nitrogen (N0), and low phosphorus (P0) conditions. The grain yield and panicle number per plant were measured for each CSSL, and their relative values based on N0 or P0 and normal conditions were considered as the measurement for tolerance to low soil nutrient. The results showed that both the N0 and P0 conditions had strong negative effect on yield and panicle number, and there were different responses among the CSSLs to the stresses, and the relative traits had a significant negative correlation with the traits under the normal condition. 9311 showed better tolerance to low nutrient conditions than Nipponbare. A total of 38 chromosomal regions or quantitative trait loci (QTLs) all with negative allelic effects from Nipponbare were detected for the measured traits under the nitrogen and the phosphorus stresses, of those 26 QTLs were for the yield and panicle number, 12 QTLs for the relative traits. Five chromosomal regions were identified in common under both the stresses, while most QTLs (81%) were specifically detected only in low nitrogen or phosphorus condition. Such different QTLs suggest that the responses to limiting nitrogen and phosphorus conditions are regulated by different sets of genes in rice. Most QTLs for the relative traits were co-localized with those for the yield and the panicle number under either nitrogen or phosphorus stresses, indicating that the tolerance QTLs may be involved in nitrogen and phosphorus uptake or assimilation pathway in rice.

Key words: Oryza sativa L., Low nitrogen and phosphorus tolerance, Chromosomal segment substitution lines(CSSLs), Quantitative trait locus(QTL)

[1]Li Q-K(李庆逵), Zhu Z-L(朱兆良), Yu T-R(于天仁). The Fertilizer Problems for Sustainable Development of Agriculture in China (中国农业持续发展中的肥料问题). Nanchang: Jiangxi Science and Technology Press, l997. pp 38–51 (in Chinese)
[2]Zhang Q. Strategies for developing Green Super Rice. Proc Natl Acad Sci USA, 2007, 104: 16402–16409
[3]Hirel B, Bertin P, Quillere I, Bourdoncle W, Attagnant C, Dellay C, Gouy A, Cadiou S, Retailliau C, Falque M, Gallais A. Towards a better understanding of the genetic and physiological basis for nitrogen use efficiency in maize. Plant Physiol, 2001, 125:1258–1270
[4]Loudet O, Chaillou S, Merigout P, Talbotec J, Daniel-Vedele F. Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis. Plant Physiol, 2003, 131: 345–358
[5]Liang Q(梁泉), Liao H(廖红), Mei M-T(梅曼彤), Cheng X-H(程小会), Yan X-L(严小龙). Research progress in QTL analysis of traits related to phosphorus efficiency in crops. Mol Plant Breed (分子植物育种), 2006, 4(4):453–463 (in Chinese with English abstract)
[6]Ni J, Wu P, Senadhira D, Huang N. Mapping QTLs for phosphorus deficiency tolerance in rice (Oryza sativa L.). Theor Appl Genet, 1998, 97:1361–1369
[7]Wissuwa M, Wegner J, Ae N, Yano M. Substitution mapping of Pup1: A major QTL increasing phosphorus uptake of rice from a phosphorus-deficient soil. Theor Appl Genet, 2002, 105: 890–897
[8]Tong H H, Mei H W, Yu X Q, Xu X Y, Li M S, Zhang S Q, Luo L J. Identification of related QTL at late developmental stage in rice (Oryza sativa L.) under two nitrogen levels. Acta Genet Sin, 2006, 33: 458–467
[9]Mu P(穆平), Huang C(黄超), Li J-X(李君霞), Liu L-F(刘立峰), Li Z-C(李自超). Yield trait variation and QTL mapping in a DH population of rice under phosphorus deficiency. Acta Agron Sin (作物学报), 2008, 34(7): 1137–1142 (in Chinese with English abstract)
[10]Yamaya T, Obara M, Nakajima H, Sasaki S, Hayakawa T, Sato T. Genetic manipulation and quantitative trait loci mapping for nitrogen recycling in rice. J Exp Bot, 2002, 53: 917–925
[11]Lian X, Xing Y, Yan H, Xu C, Zhang Q. QTLs for low nitrogen tolerance at seedling stage identified using a recombinant inbred line population derived from an elite rice hybrid. Theor Appl Genet, 2005, 112: 85–96
[12]Ashikari M, Matsuoka M. Identification, isolation and pyramiding of quantitative trait loci for rice breeding. Trends Plant Sci, 2006, 11: 344–350
[13]Zamir D. Improving plant breeding with exotic genetic libraries. Nat Rev Genet, 2001, 2: 983–989
[14]Xu H-S(徐华山), Sun Y-J(孙永建), Zhou H-J(周红菊), Yu S-B(余四斌). Development and characterization of contiguous segment substitution lines with background of elite restorer line. Acta Agron Sin (作物学报), 2007, 33(6): 979–986 (in Chinese with English abstract)
[15]Yang D(杨德). Design and Analysis of Experiments (试验设计与分析)．Beijing: China Agriculture Press，2002. pp 357–360 (in Chinese)
[16]StatSoft. Statistica. StatSoft Incorporated, Tusla, Oklahoma, 1997
[17]Young N D, Tanksley S D. Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet, 1989, 77: 95–101
[18]Eshed Y, Zamir D. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics, 1995, 141: 1147–1162
[19]Piao Z-Z(朴钟泽), Han L-Z(韩龙植), Koh L-J (高熙宗), Lu J-A(陆家安), Zhang J-M(张建明). Selection effect of nitrogen use efficiency in rice. Acta Agron Sin (作物学报), 2004, 30(7): 651–656 (in Chinese with English abstract)
[20]Li F(李锋), Pan X-H(潘晓华), Liu S-Y(刘水英), Li M-Y(李木英), Yang F-S(杨福孙). Effect of phosphorus deficiency stress on root morphology and nutrient absorption of rice cultivars. Acta Agron Sin (作物学报), 2004, 30(5): 438–442 (in Chinese with English abstract)
[21]Zeng J-M (曾建敏), Cui K-H(崔克辉), Huang J-L(黄见良), He F(贺帆), Peng S-B(彭少兵). Responses of physio-biochemical properties to N-fertilizer application and its relationship with nitrogen use efficiency in rice (Oryza sativa L.). Acta Agron Sin (作物学报), 2007, 33 (7):1168–1176 (in Chinese with English abstract)
[22]Geloff G C. Intact-plant screening for tolerance of nutrient deficiency stress. Plant Soil, 1987, 99: 3–16
[23]Cheng J-F(程建峰), Dai T-B(戴廷波), Cao W-X(曹卫星), Jiang D(姜东). Classification, identification and evaluation of nitrogen nutrition efficiencies in rice germplasms at seedling stage. Acta Agron Sin (作物学报), 2005, 31(12):1640–1647(in Chinese with English abstract)
[24]Hang N-R(黄农荣), Zhong X-H(钟旭华), Zheng H-B(郑海波). Selection of rice genotypes with high nitrogen utilization efficiency and its evaluation indices. Chin Agric Sci Bul (中国农学通报), 2006, 22(6): 29–34 (in Chinese with English abstract)
[25]Liu Y(刘亚), Li Z-C(李自超), Mi G-H(米国华), Zhang H-L(张洪亮), Mu P(穆平), Wang X-K(王象坤). Screening and identification for tolerance to low-phosphorus stress of rice germplasm (Oryza sativa L.). Acta Agron Sin (作物学报), 2005, 31(2): 238–242 (in Chinese with English abstract)
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