Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 1055-1060.doi: 10.3724/SP.J.1006.2010.01055

• RESEARCH ACTIVITIES • Previous Articles    

Effects of Phosphorus Fertilizer on Accumulation of High Molecular Weight Glutenin Subunits and Glutenin Macropolymer Size Distribution in Wheat Grain

NI Ying-Li1,WANG Zhen-Lin1*,LI Wen-Yang12,YAN Su-Hui1,2,YIN Yan-Ping1,LI Yong1,WANG Ping1,CHEN Xiao-Guang1   

  1. 1National Key Laboratory of Crop Biology/Agronomy College,Shandong Agricultural University,Tai'an 271018,China;2College of Plant Science,Anhui Science and Technology University,Fengyang 233100,China
  • Received:2009-12-08 Revised:2010-03-19 Online:2010-06-12 Published:2010-04-14
  • Contact: WANG Zhen-Lin,E-mail: zlwang@sdau.edu.cn; zlwangsd@sina.com; Tel: 0538-8241359


For gaining insights into the relationship between the accumulation of high-molecular-weight glutenin subunit (HMW-GS) and the distribution of glutenin macropolymer (GMP) in wheat (Triticum aestivum L.), Shannong 12, a cultivar with high glutenin, was used in a field experiment with four phosphorus (P) application levels (0, 40, 100, and 160 kg ha-1) under low nitrogen (112.5 kg ha-1) and normal nitrogen (225 kg ha-1) conditions, respectively. The result showed that HMW-GS in grains formed before 14 d after anthesis, and its content at maturity was higher in P application treatments than in the treatment with no P fertilizer applied. Phosphorus was in favor of the accumulation of HMW-GS under the low nitrogen (N) condition, but caused a negative effect in high P levels under the normal N condition. The content of GMP particle in <10 μm in diameter was significantly reduced with increasing P application. The effect increased with increasing P level under the low N condition, but showed the largest reduction at P level of 100 kg ha-1 and the smallest reduction at P level of 40 kg ha-1. For GMP particle in 10–100 μm and >100 μm groups, their contents tended to increasing with more P applied under both N conditions. Under the normal N condition, there was no significant effect of P level on the number distribution of GMP particle. At maturity, the content of HMW-GS was negatively correlated with the volume of GMP particle in <10 μm group, but positively correlated with that in 10–100 µm group. The result suggested that larger GMP particles have higher HMW-GS content than the smaller GMP particles.

Key words: Wheat, High-molecular-Weight glutenin subunit(HMW-GS), Glutenin macropolymer(GMP), Phosphorus, Nitrogen

[1]Steffolani M E, Perez G T, Ribotta P D, Puppo M C, Leon A E. Effect of transglutaminase on properties of glutenin macropolymer and dough rheology
[J]. J Cereal Sci
[2]Don C, Lichtendonk W J, Plijter J J, Hamer R J. Glutenin macropolymer: A gel formed by glutenin particle
[J].J Cereal Sci
[3]Don C, Lichtendonk W J, Plijter J J, Hamer R J. Understanding the link between GMP and dough: from glutenin particle in flour towards developed dough
[J]. J Cereal Sci
[4]Bean S R, Lyne R K, Tilley K A, Chung O K, Lookhart G L. A rapid method for quantitation of insoluble polymeric proteins in flour
[J].Cereal Chem
[5]Gupta R B, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheat: Effect of variation in thequantity and size distribution of polymeric protein
[J].J Cereal Sci
[6]Zhu J, Khan K. Characterization of glutenin protein fractions from sequential extraction of hard red spring wheats of different bread-making quality
[J].Cereal Chem
[7]Sun H(孙辉), Yao D-N(姚大年), Li B-Y(李保云), Liu G-T(刘广田), Zhang S-Z(张树臻). Correlation between content of glutenin macropolymer (GMP) in wheat and baking quality. Chin Cereals Oils Assoc (中国粮油学报), 1998, 13(6): 13-16 (in Chinese with English abstract)
[8]Don C G, Mann F B, Hamer R J. HMW-GS affect the properties of glutenin particles in GMP and thus flour quality
[J]. J Cereal Sci
[9]Liang R-Q(梁荣奇), Zhang Y-R(张义荣), You M-S(尤明山), Mao S-F(毛善锋), Song J-M(宋建民), Liu G-T(刘广田). Multistacking SDS-PAGE for wheat glutenin polymer and its relation to bread making quality. Acta Agron Sin (作物学报), 2002, 28(5): 609-614(in Chinese with English abstract)
[10]Payne P I. Relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties
[J].J Sci Food Agric
[11]Zhao Y-M(赵友梅), Wang S-J(王淑俭). The application of HMW glutenin subunits in the study of wheats baking quality property. Acta Agron Sin (作物学报), 1990, 16(3): 208-218 (in Chinese with English abstract)
[12]Shi Y(石玉), Zhang Y-L(张永丽), Yu Z-W(于振文). Contents of grain protein components and their relationships to processing quality in wheat
[J].Acta Agron Sin (作物学报.2009, 35(7):1306-1312
[13]Zhu J B, Khan K. Characterization of monomeric and glutenin polymeric proteins of hard red spring wheats during grain development by multistacking SDS-PAGE and capillary zone electrophoresis
[J].Cereal Chem
[14]Deng Z-Y(邓志英), Tian J-C(田纪春), Liu X-P(刘现鹏). Accumulation regularity of protein components in wheat cultivars with different HMW-GS. Acta Agron Sin (作物学报), 2004, 30(5): 481-486 (in Chinese with English abstract)
[15]Yue H-W(岳鸿伟), Qin X-D(秦晓东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星), Jiang D(姜东). Effects of nitrogen rate on accumulations of HMW-GS and GMP in wheat grain. Acta Agron Sin (作物学报), 2006, 32(10): 1678-1683 (in Chinese with English abstract)
[16]Deng Z, Tian J, Zhao L, Zhang Y, Sun C. High temperature-induced changes in high molecular weight glutenin subunits of Chinese winter wheat and its influences on the texture of Chinese noodles
[J].J Agron Crop Sci
[17]DuPont F M, Hurkman W J, Vensel W H, Tanaka C, Kothari K M, Chung O K. Protein accumulation and composition in wheat grains: Effects of mineral nutrients and high temperature
[J].Eur J Agron
[18]Spiertz J H J, Hamer R J, Xu H, Primo-Martin C, Don C, van der Putten P E L. Heat stress in wheat (Triticum aestivum L.): Effects on grain growth and quality traits. Eur J Agron, 2006, 25: 89-95
[19]Jiang D, Yue H, Wollenweber B, Tan W, Mu H, Bo Y, Dai T, Jing Q, Cao W. Effects of post-anthesis drought and waterlogging on accumulation of high molecular weight glutenin subunits and glutenin macropolymer size distribution in wheat grain. J Agron Crop Sci, 2009, 5: 89-97
[20]Zhang P P, He Z H, Zhang Y, Xia X C. Association between %SDS-unextractable polymeric protein (%UPP) and end-use quality in Chinese bread wheat cultivars
[J]. Cereal Chem
[21]Pei X-X(裴雪霞), Zhang D-Y(张定一), Wang J-A(王姣爱). Effect of N and P fertilizer on the product and quality of high quality and gluten wheat. Agric Res Arid Areas (干旱地区农业研究), 2003,21(3): 48-50 (in Chinese with English abstract)
[22]Weegels P L, van de Pijpekamp A M, Graveland A, Hamer R J, Schofield J D. Depolymerisation and re-polymerisation of wheat glutenin during dough processing: I. Relationships between glutenin macropolymer content and quality parameters. J Cereal Sci, 1996, 23: 103-111
[23]Zheng P Y(郑丕尧). Introduction of crop physiology. Beijing: Beijing Agricultural University Press, 1992. p 198
[24]Wang X-D(王旭东), Yu Z-W(于振文). Effects of phosphorus fertilizer on the product and quality of high quality and gluten wheat. Shandong Agric Sci (山东农业科学), 2003, (6): 35-36 (in Chinese)
[25]Zhao G-C(赵广才), He Z-H(何中虎), Liu L-H(刘利华). Study on the coenhancing regulating effect of fertilization and watering on the main quality and yield in Zhongyou 9507 high gluten wheat. Sci Agric Sin (中国农业科学), 2004, 37(3): 351-356 (in Chinese with English abstract)
[26]Gupta R B, Batey I L, MacRitchie F. Relationship between protein composition and functional properties of wheat flour. Cereal Chem, 1992, 69: 125-131
[27]Zhao H-X(赵惠贤), Hu S-W(胡胜武), Ji W-Q(吉万全), Xue X-Z(薛秀庄), Guo A-G(郭蔼光), Mares D. Study on relationship between the size distribution of glutenin polymeric protein and wheat flour mixing properties. Sci Agric Sin (中国农业科学), 2001,34(5): 465-468 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] QIN Lu, HAN Pei-Pei, CHANG Hai-Bin, GU Chi-Ming, HUANG Wei, LI Yin-Shui, LIAO Xiang-Sheng, XIE Li-Hua, LIAO Xing. Screening of rapeseed germplasms with low nitrogen tolerance and the evaluation of its potential application as green manure [J]. Acta Agronomica Sinica, 2022, 48(6): 1488-1501.
[3] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[4] PENG Xi-Hong, CHEN Ping, DU Qing, YANG Xue-Li, REN Jun-Bo, ZHENG Ben-Chuan, LUO Kai, XIE Chen, LEI Lu, YONG Tai-Wen, YANG Wen-Yu. Effects of reduced nitrogen application on soil aeration and root nodule growth of relay strip intercropping soybean [J]. Acta Agronomica Sinica, 2022, 48(5): 1199-1209.
[5] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[6] YAN Yu-Ting, SONG Qiu-Lai, YAN Chao, LIU Shuang, ZHANG Yu-Hui, TIAN Jing-Fen, DENG Yu-Xuan, MA Chun-Mei. Nitrogen accumulation and nitrogen substitution effect of maize under straw returning with continuous cropping [J]. Acta Agronomica Sinica, 2022, 48(4): 962-974.
[7] LI Xin-Ge, GAO Yang, LIU Xiao-Jun, TIAN Yong-Chao, ZHU Yan, CAO Wei-Xing, CAO Qiang. Effects of sowing dates, sowing rates, and nitrogen rates on growth and spectral indices in winter wheat [J]. Acta Agronomica Sinica, 2022, 48(4): 975-987.
[8] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[9] ZHOU Yue, ZHAO Zhi-Hua, ZHANG Hong-Ning, KONG You-Bin. Cloning and functional analysis of the promoter of purple acid phosphatase gene GmPAP14 in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 590-596.
[10] YUAN Jia-Qi, LIU Yan-Yang, XU Ke, LI Guo-Hui, CHEN Tian-Ye, ZHOU Hu-Yi, GUO Bao-Wei, HUO Zhong-Yang, DAI Qi-Gen, ZHANG Hong-Cheng. Nitrogen and density treatment to improve resource utilization and yield in late sowing japonica rice [J]. Acta Agronomica Sinica, 2022, 48(3): 667-681.
[11] DING Hong, XU Yang, ZHANG Guan-Chu, QIN Fei-Fei, DAI Liang-Xiang, ZHANG Zhi-Meng. Effects of drought at different growth stages and nitrogen application on nitrogen absorption and utilization in peanut [J]. Acta Agronomica Sinica, 2022, 48(3): 695-703.
[12] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[13] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[14] WANG Yan, CHEN Zhi-Xiong, JIANG Da-Gang, ZHANG Can-Kui, ZHA Man-Rong. Effects of enhancing leaf nitrogen output on tiller growth and carbon metabolism in rice [J]. Acta Agronomica Sinica, 2022, 48(3): 739-746.
[15] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
Full text



No Suggested Reading articles found!