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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 1055-1060.doi: 10.3724/SP.J.1006.2010.01055

• RESEARCH ACTIVITIES • Previous Articles    

Effects of Phosphorus Fertilizer on Accumulation of High Molecular Weight Glutenin Subunits and Glutenin Macropolymer Size Distribution in Wheat Grain

NI Ying-Li1,WANG Zhen-Lin1*,LI Wen-Yang12,YAN Su-Hui1,2,YIN Yan-Ping1,LI Yong1,WANG Ping1,CHEN Xiao-Guang1   

  1. 1National Key Laboratory of Crop Biology/Agronomy College,Shandong Agricultural University,Tai'an 271018,China;2College of Plant Science,Anhui Science and Technology University,Fengyang 233100,China
  • Received:2009-12-08 Revised:2010-03-19 Online:2010-06-12 Published:2010-04-14
  • Contact: WANG Zhen-Lin,E-mail: zlwang@sdau.edu.cn; zlwangsd@sina.com; Tel: 0538-8241359

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Abstract:

For gaining insights into the relationship between the accumulation of high-molecular-weight glutenin subunit (HMW-GS) and the distribution of glutenin macropolymer (GMP) in wheat (Triticum aestivum L.), Shannong 12, a cultivar with high glutenin, was used in a field experiment with four phosphorus (P) application levels (0, 40, 100, and 160 kg ha-1) under low nitrogen (112.5 kg ha-1) and normal nitrogen (225 kg ha-1) conditions, respectively. The result showed that HMW-GS in grains formed before 14 d after anthesis, and its content at maturity was higher in P application treatments than in the treatment with no P fertilizer applied. Phosphorus was in favor of the accumulation of HMW-GS under the low nitrogen (N) condition, but caused a negative effect in high P levels under the normal N condition. The content of GMP particle in <10 μm in diameter was significantly reduced with increasing P application. The effect increased with increasing P level under the low N condition, but showed the largest reduction at P level of 100 kg ha-1 and the smallest reduction at P level of 40 kg ha-1. For GMP particle in 10–100 μm and >100 μm groups, their contents tended to increasing with more P applied under both N conditions. Under the normal N condition, there was no significant effect of P level on the number distribution of GMP particle. At maturity, the content of HMW-GS was negatively correlated with the volume of GMP particle in <10 μm group, but positively correlated with that in 10–100 µm group. The result suggested that larger GMP particles have higher HMW-GS content than the smaller GMP particles.

Key words: Wheat, High-molecular-Weight glutenin subunit(HMW-GS), Glutenin macropolymer(GMP), Phosphorus, Nitrogen

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