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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (02): 280-285.doi: 10.3724/SP.J.1006.2011.00280


Characterization and Gene Mapping of a Spikelet Mutant multi-floret 1(mf1) in Rice

LI Yun-Feng,YANG Zheng-Lin,LING Ying-Hua,WANG Nan,REN De-Yong,WANG Zeng,HE Guang- Hua*   

  1. Rice Research Institute, Southwest University / Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture, Chongqing 400716, China
  • Received:2010-07-08 Revised:2010-10-05 Online:2011-02-12 Published:2010-12-15
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

Abstract:  Rice has a determinate spikelet producing a fertile floret above two sterile lemmas. In this study, we reported a multi-floret 1 (mf1) mutant, in which spikelet lost the determinacy and produced two or more florets in an alternate phyllotaxy above sterile lemmas. In addition, all the florets showed the defects of floral organs development, such as the elongated leafy lemma, and decreased lodicule/stamen. Genetic analysis indicated that the mf1 trait is controlled by a single recessive gene. By bulked segregation analysis (BSA) and rice SSR molecular maker, the mf1 locus was located between PSSR3 and RM7576 on chromosome 3 with a 34 kb physical distance containing four annotated genes. This result provided a foundation of map-based cloning and function analysis of MF1 gene.

Key words: Rice, Spikelet, multi floret 1, Determinacy

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