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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1683-1688.doi: 10.3724/SP.J.1006.2011.01683

• RESEARCH NOTES • Previous Articles     Next Articles

Identification of Yd2 Genotype in Barley with Molecular Markers and Their Application in Molecular Marker-assisted Selection

ZHAO Yan-Hong1,*,WANG Yan-Fang1,LI Run-Zhi2,*,NIU Hong-Bin3,XUE Jing-Ai2,LIU Lin-De1   

  1. 1School of Life Science, Ludong University, Yantai, 264025, China; 2College of Agronomy, Shanxi Agricultural University, Taigu, 030801, China; 3National Engineering Research Center for Wheat, Zhenzhou 450002, China?
  • Received:2011-02-16 Revised:2011-05-20 Online:2011-09-12 Published:2011-06-28
  • Contact: 赵彦宏, E-mail: zyhbob@163.com, Tel: 13153512601; 李润植, E-mail: rli2001@hotmail.com

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Abstract: The objectives of this study were to assess the feasibility of molecular markers YLM, CAPS-Ylp, and ASPCR-Ylp used in Yd2 gene identification in barley and to provide a rapid and effective marker-assisted selection (MAS) method for facilitating the application of Yd2 gene in resistance breeding to barley yellow dwarf virus (BYDV) in barley. The three markers were used to detect the Yd2 genotypes in fifty-two barley cultivars (lines) and four hybrids of F1 generation. The resistance identification was performed to confirm the Yd2 genotypes of these cultivars (lines). Moreover, the three markers were applied to select the plants with Yd2 gene in a practice resistant-breeding program. The three markers could identify successfully the Yd2 genotypes of twenty barley lines and four F1 hybrids with known Yd2 genotypes. This result confirmed that the effectiveness of the three markers in identification of Yd2 genotypes in barley. In 32 barley cultivars (lines) with unknown Yd2 genotype, the three markers revealed Yd2-/Yd2- genotype in 27 cultivars (lines) and Yd2+/Yd2+ genotype in the remaining five cultivars (lines). In a breeding practice aiming at BYDV resistance improvement, 16 positive plants with Yd2+/Yd2+ genotype were selected rapidly and successfully from the BC2F2 population using MAS approach. We suggest the cooperative application of the three markers in selecting Yd2 gene and genotype from a large scale of lines in barley backcross populations to ensure the accuracy and efficiency of MAS.

Key words: Barley, Barley yellow dwarf disease, Yd2 gene, Marker-assisted selection, Molecular marker

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