Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 168-173.doi: 10.3724/SP.J.1006.2012.00168

• RESEARCH NOTES • Previous Articles     Next Articles

Analysis of Phenotype and Physiology together with Mapping of a Leaf Apex Dead Gene (lad) in Rice (Oryza sativa L.)

DU Qing,FANG Li-Kui,SANG Xian-Chun,LING Ying-Hua,LI Yun-Feng,YANG Zheng-Lin,HE Guang-Hua,ZHAO Fang-Ming*   

  1. Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety of Genetically Modified Crops, Chongqing 400716, China?
  • Received:2011-05-19 Revised:2011-09-18 Online:2012-01-12 Published:2011-11-07
  • Contact: 赵芳明, E-mail: zhaofangming2004@163.com

PDF

1374

Cited

7

Abstract: Leaf plays an important role in photosynthesis. Studying the leaf premature senescence is important for increasing the economic yield of crop. A new mutant of rice leaf apex dead reported in the paper derived from a restorer line Jinhui10 treated by EMS. In the mutant, the leaf apexes appeared normal before the 5th leaf unfolded. However, the leaf apexes of all leaves except the 5th leaf exhibited yellow and gradually died when the 5th leaf fully expanded; and afterwards the apexes of new fully expanded leaves became yellow and dead. The physiological and biochemical analysis showed that the lad mutant contained lower contents of chlorophyll and soluble protein compared with the wild type control, and the activity of SOD was abnormal. Again, the plant height, leaf length, grain number and seed setting rate of the lad mutant were significantly lower than those of the control. Thepremature senescence mutant was controlled by a single recessive nuclear gene. And the LAD gene was mapped between the SSR markers SWU11-19 and SWU11-5 on the 11th chromosome, with 13 cM of the genetic distance. Furthermore, the LAD gene was co-segregated with the markers SWU11-25 and SWU11-27. The results laid a favorable foundation for further map-based cloning and functional analysis of the LAD gene.

Key words: Rice, Leaf apex dead, Gene mapping, Phenotypic analysis, Physiologic analysis

[1]Yoshida S. Molecular regulation of leaf senescence. Plant Boil, 2003, 6: 79–84
[2]Nam H G. The molecular genetic analysis of leaf senescence. Curr Opin Biotechnol, 1997, 8: 200–207
[3]Duan J(段俊), Liang C-Y(梁成邺), Huang Y-W(黄毓文). Studies on leaf Senescence of hybrid rice at flowering and grain formation stage. Acta Physiol Sin, 1997, 23(2): 139–144 (in Chinese with English abstract)
[4]Doelling J H, Walker J M, Friedman E M, Thompson A R, Vierstra R D. The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and in Arabidopsis thaliana. J Biol Chem, 2002, 277: 33105–33114
[5]Hanaka H, Takashi N, Yumiko S, Tomohiko K, Hiroaki H, Daisuke S, Satoshi T, Yoshinori O. Leaf senescence and starvation-induced chlorosis are accelerated by the disruption of an Arabidopsis autophagy gene. Plant Physiol, 2002, 129: 1181–1193
[6]Yoshida S, Ito M, Nishida I, Watanabe A. Identification of a novel gene HYS1/CPR5 that has a repressive role in the induction of leaf senescence and pathogendefence responses in Arabidopsis thaliana. Plant J, 2002, 29: 427–437
[7]Jing H C, Sturre M J G, Hille J, Dijkwel P P. Arabidopsis onset of leaf death mutants identify a regulatory pathway controlling leaf senescence. Plant J, 2002, 32: 51–63
[8]Gribic V, Bleecker A B. Ethylene regulates the tinming of leaf senescence in Arabidopsis. Plant, 1995, 8: 595–602
[9]Oh S A, Park J H, Lee G I, Paek K H, Park S K, Nam H G. Identification of three genetic loci controlling leaf senescence in Arabidopsis thaliana. Plant J, 1997, 12: 527–535
[10]Woo H R, Goh C H, Park J H, de la Serve B T, Kim J H, Park Y I, Nam H G. Extended leaf longevity in the ore4-1 mutant of Arabidopsis with a reduced expression of a plastid ribosomal protein gene. Plant J, 2002, 31: 331–340
[11]Woo H R, Chung K M, Park J H, Oh S A, Ahn T, Hong S H, Jang S K, Nam H G. ORE9, an F-box protein that regulates leaf senescence in Arabidopsis. Plant Cell, 2001, 13: 1779–1790
[12]Wang J, Wu S J, Zhou L H, Xu J F, Hu J, Fang Y X, Gu M H, Liang G H. Genetic analysis and molecular mapping of a presenescing leaf gene psl1 in rice (Oryza sativa L.). Chin Sci Bull, 2006, 51: 2986–2992
[13]Li F Z, Hu G C, Fu Y P, Si H M, Sun Z X. Genetic analysis and high-resolution mapping of a premature senescence gene Pse(t) in rice(Oryza sativa L.). Genome, 2006, 48: 738–746
[14]Zhu L, Liu W Z, Wu C, Luan W J, Fu Y P, Hu G C, Si H M, Sun Z X. Identification and fine mapping of a gene related to pale green leaf phenotype near centromere region in rice (Oryza sativa). Rice Sci, 2007, 14: 172–180
[15]Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H. Genetic analysis and gene mapping of dominant presenescing leaf gene PSL3 in rice (Oryza sativa). Chin Sci Bull, 2010, 55(23): 2517–2521
[16]Liang J-S(梁建生), Cao X-Z(曹显祖). Studies on the relationship between several physiological characteristics of leaf and bleeding rate of roots in hybrid rice. J Jiangsu Agric Coll (江苏农学院学报), 1993, 14(4): 25–30 (in Chinese with English abstract)
[17]Aenon D I. Copper enzymes in isolated chloroplasts: polyphenol oxidase in beta vulgaris. Plant Physiol, 1949, 24: 1–15
[18]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Plant Physiol, 1994, 144: 307–313
[19]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69–76
[20]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)
[21]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene (RL10(t)) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817
[22]Christine H F, Vanacker H, Leonardo D G, Jeremy H. Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal chilling temperatures: review. Plant Physiol Biochem, 2002, 40: 659–668
[23]Guo P-G(郭培国), Li M-Q(李明启). Studies on photosynthetic characteristics in rice hybrid progenies and their parents: I. Chlorophyll content, chlorophyll-protein complex and chlorophyll fluorescence kinetics. J Trop Subtrop Bot (热带亚热带植物学报), 1996, 4(4): 60–65 (in Chinese with English abstract)
[24]Biswas A K, Choudhuri M A. Mechanism of monocarpic senescence in rice. Plant Physiol, 1980, 65: 340–345
[25]Quirino B F, Noh Y S, Himelblau E, Amasino R M. Molecular aspects of leaf senescence. Trends Plant Sic, 2000, 5: 278–282
[26]Robatzek S, Somssich I E. A new member of the Arabidopsis WRKY transcription factor family, at WRKY6, is associated with both senescence and defense-related processes. Plant J, 2001, 28: 123–133
[27]Miao Y, Laun T, Zimmermann P, Zentgraf U. Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis thaliana. Plant Mol Biol, 2004, 55: 853–867
[28]Jiang H W, Li M R, Liang N T, Yan H B, Wei Y B, Xu X L, Liu J, Xu Z F, Chen F, Wu G J. Molecular cloning and function analysis of the stay green gene in rice. Plant J, 2007, 52: 197–209
[29]Lee R H, Wang C H, Huang L T, Chen S C G. Leaf senescence in rice plants: cloning and characterization of senescence up-regulated genes. J Exp Bot, 2001, 52: 1117–1121
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
[15] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd