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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (01): 168-173.doi: 10.3724/SP.J.1006.2012.00168

• RESEARCH NOTES • Previous Articles     Next Articles

Analysis of Phenotype and Physiology together with Mapping of a Leaf Apex Dead Gene (lad) in Rice (Oryza sativa L.)

DU Qing,FANG Li-Kui,SANG Xian-Chun,LING Ying-Hua,LI Yun-Feng,YANG Zheng-Lin,HE Guang-Hua,ZHAO Fang-Ming*   

  1. Rice Research Institute, Southwest University / Chongqing Key Laboratory of Application and Safety of Genetically Modified Crops, Chongqing 400716, China?
  • Received:2011-05-19 Revised:2011-09-18 Online:2012-01-12 Published:2011-11-07
  • Contact: 赵芳明, E-mail: zhaofangming2004@163.com

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Abstract: Leaf plays an important role in photosynthesis. Studying the leaf premature senescence is important for increasing the economic yield of crop. A new mutant of rice leaf apex dead reported in the paper derived from a restorer line Jinhui10 treated by EMS. In the mutant, the leaf apexes appeared normal before the 5th leaf unfolded. However, the leaf apexes of all leaves except the 5th leaf exhibited yellow and gradually died when the 5th leaf fully expanded; and afterwards the apexes of new fully expanded leaves became yellow and dead. The physiological and biochemical analysis showed that the lad mutant contained lower contents of chlorophyll and soluble protein compared with the wild type control, and the activity of SOD was abnormal. Again, the plant height, leaf length, grain number and seed setting rate of the lad mutant were significantly lower than those of the control. Thepremature senescence mutant was controlled by a single recessive nuclear gene. And the LAD gene was mapped between the SSR markers SWU11-19 and SWU11-5 on the 11th chromosome, with 13 cM of the genetic distance. Furthermore, the LAD gene was co-segregated with the markers SWU11-25 and SWU11-27. The results laid a favorable foundation for further map-based cloning and functional analysis of the LAD gene.

Key words: Rice, Leaf apex dead, Gene mapping, Phenotypic analysis, Physiologic analysis

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