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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 21-28.doi: 10.3724/SP.J.1006.2013.00021

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development of Gossypium barbadense Chromosome 18 Segment Substitution Lines in the Genetic Standard Line TM-1 of Gossypium hirsutum and Mapping of QTLs Related to Agronomic Traits

FU Yang,YUAN Dong-Dong,HU Wen-Jing,CAI Cai-Ping,GUO Wang-Zhen*   

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-05-02 Revised:2012-09-05 Online:2013-01-12 Published:2012-11-15
  • Contact: 郭旺珍, E-mail: moelab@njau.edu.cn

Abstract:

Substitution line 18 (Sub18) has the background of genetic standard line of G. hirsutum acc. TM-1 with the 18th chromosome of G. barbadense accession 3-79. In the study, a set of G. barbadense accession 3-79 chromosome 18 segment substitution lines (CSSL18) were developed via molecular marker-assisted selection (MAS), with TM-1 as a recipient parent and Sub18 as a donor. The developed CSSLs consisted of 45 lines and 78 introgressed segments in total. Of them, 27 were introgressed with single segment, accounting for 60% of the total lines; 9 were introgressed with two segments, accounting for 20%; and 9 were introgressed with three or more segments, accounting for 20%. The total length of introgression segments was 467.6 cM, which is 4 times of the genetic length of the chromosome 18. The substituted segment length varied in each line, ranging from the shortest of 0.9 cM to the longest of 20.35 cM, with an average length of 5.99 cM. Further, the opening bud trait was detected in 13 substitution lines, with the shortest introgressed segment of 5.05 cM. Using TM-1, Sub18 and 45 substituted lines as materials, we investigated agronomic traits and carried out QTL tagging by joint positioning analysis. Seven additive effect QTLs for fiber strength (qFS-C18-1), uniformity (qFU-C18-1), micronaire (qFMi-C18-1), maturity (qFMa-C18-1), lint weight (qLW-C18-1), seed index (qSI-C18-1), and lint percentage (qLP-C18-1) and five epistatic effect QTLs were identified, respectively. The results will lay a foundation for the fine mapping and cloning of target QTLs, and the molecular breeding by design for pyramiding multi-traits in cotton.

Key words: Chromosome segment substitution lines, Molecular marker, QTL, TM-1, Sub18, Opening bud

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