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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1366-1376.doi: 10.3724/SP.J.1006.2013.01366


Excavation of Neutral Alleles San, Sbn, and Scn from the Rice Germplasm Harboring S5n Gene

LI Hong-Yan**,WANG Si-Zhe**,SHAHID Muhammad Qasim**,CHEN Zhi-Xiong,WANG Lan,CHEN Feng-Yi,LIU Xiang-Dong*,LU Yong-Gen*   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China?
  • Received:2012-11-30 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 刘向东, E-mail: xdliu@scau.edu.cn; 卢永根, yglu@scau.edu.cn


One of the principal barriers in utilization of heterosis between indica and japonica subspecies of Asian cultivated rice is F1 pollen sterility. According to the “specific compatibility gene” theory, there are at least six loci, which cause indica and japonica hybrid pollen sterility. Excavation and utilization of neutral alleles for overcoming F1 hybrid sterility have great importance in hybrid breeding. Varieties carrying S5n gene were used to cross with Taichung 65 and its near isogenic lines (NILs) to produce F1 hybrids with single-locus interaction, double-loci interaction and triple-loci interaction. Pollen fertility and seed setting rate of these hybrids were observed in F1 to pre-judge the existence of neutral alleles and then F2generation was genotyped with molecular markers tightly linked to Sa, Sb and Scloci to verify the presence of San, Sbn and Scn by detecting segregation ratio. The results showed that two materials (Huibeizi and Madhukar) carried San and Sbn, three materials (Fanhaopi, Biwusheng and YuetaiB) carried Sbn and one material (Jackson) carried Scn. These materials have wide compatibility and neutral alleles are important germplasm for hybrid breeding and knowing the molecular mechanisms underlying hybrid male sterility.

Key words: Rice (Oryza sativa L.), Allelic interaction, Hybrid sterility, Male sterility, Wide compatibility gene (S5n)

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