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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (01): 51-57.doi: 10.3724/SP.J.1006.2016.00051

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Regulation of DTA-6 by Abscission Cellulase and GmAC Gene Expression in Flowers and Pods of Soybean

CUI Hong-Qiu1,2,**,FENG Nai-Jie1,**,SUN Fu-Dong1,LIU Tao1,LI Jian-Ying2,DU Ji-Dao1,HAN Yi-Qiang1,ZHEN Dian-Feng1,*   

  1. 1Agronomy of College / Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2 Daqing branch College / Heilongjiang Academy of Agricultural Science, Daqing163316, China
  • Received:2015-02-28 Revised:2015-09-06 Online:2016-01-12 Published:2015-10-08
  • Contact: 郑殿峰, E-mail: dianfeng69@gmail.com E-mail:cuihongqiu@126.com
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (31171503, 31271652), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2012BAD20B04), Heilongjiang Provincial Graduate Science and Technology Innovation Projects (YJSCX2012-253HLJ), Heilongjiang Province Funds for Distinguished Young Scientists (JC201309), and Key Scientific and Technological Projects of Heilongjiang Agriculture and Reclamation Bureau (HNK12A-06-03, HNK12A-09-02).

Abstract:

It is effective to regulate abscission of soybean flowers and pods by spraying plant growth regulators (PGRs). This study was carried out to determine the effect of DTA-6 on abscission cellulose (AC) activity, abscission cellulose (GmAC) gene expressive, abscission of flowers and pods and yield in soybean. DTA-6 was foliage sprayed at R1 stage on three varieties of Suinong 28 (SN28), Kenfeng 16 (KF16), and Hefeng 50 (HF50) in 2012 and 2013. Our results demonstrated that DTA-6 treatment inhibited GmAC gene expression in abscission zone of flower and pod, with the maximum reduction of 51% among three varieties compared with control. The abscission cellulase activity was periodically decreased by DTA-6, with different decrements among soybean cultivars. DTA-6 significantly decreased (P<0.05) abscission rate of soybean flowers and pods. And significantly increased yield (P<0.05). The relative expression of abscission cellulase gene (GmAC) in abscission zone of flowers-pods was decreased and AC activity was regulated by DTA-6, resulting in reduced abscission rate of soybean flowers and pods and promoted yield.

Key words: DTA-6, GmAC gene expressive, Abscission cellulase, Flowers and pods abscission, Yield

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