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Acta Agron Sin ›› 2018, Vol. 44 ›› Issue (01): 144-156.doi: 10.3724/SP.J.1006.2018.00144

• RESEARCH NOTES • Previous Articles    

Pedigree Construction and SSR Analysis of Broomcorn Millet Mutant by 12C6+ Ion Beam Irradiation

LIU Tian-Peng1,DONG Kong-Jun1,DONG Xi-Cun2,HE Ji-Hong1,LIU Min-Xuan3,REN Rui-Yu1,ZHANG Lei1,YANG Tian-Yu1,4,   

  1. 1 Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 Life Sciences and Technology College, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2017-03-24 Revised:2017-09-10 Online:2018-01-12 Published:2017-09-28
  • Supported by:

    The work was supported by the Gansu Academy of Agricultural Sciences Funds for Youth and the China Agriculture Research System (CARS-07-12.5-A5).

Abstract:

To construct mutant library of physical mutation in broomcorn millet, applied five doses of 12C6+ ion beam at 50, 100, 150, 200, and 250 Gy to irradiate seed of Longmi 7 and Jinshu 9, and constructed two populations (M5) consisting of 52 and 79 lines respectively with mixed pedigree method and system cluster. The field text showed that the emergence rate of M1 decreased significantly with increasing 12C6+ dose, half lethal dose for M1 from Longmi 7 and Jinshu 9 were 150 Gy and 100 Gy. M4 at 100, 150 Gy produce a most abundant mutants. M5 and M6 Phenotypes were more stable than M4, and plant height, yield traits, plant color and seed color showed significant difference. Nine M6 lines from Longmi 7 and 11 M6 lines from Jinshu 9 were detected with six pairs of SSR primer, compared with the parents, loci variant genotype number were 1-2 and 1-4, respectively, showing abundant genetic diversity in mutant populations.

Key words: 12C6+ ion, Physical irradiation, Mutant populations, Molecular marker

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