Abstract: Soybean cyst nematode (SCN, Heterodera glycines) is a seriously destructive pest in soybean production worldwide and causes great yield loss every year. Planting resistant variety is an effective and economical method to decrease its damage. At present we obtained 41 elite soybean lines from the crosses of Hartwig × Jin 1261, Hartwig × Jin 1265 and Hartwig × Jin 1267, and genotyped them with 130 SSR markers from 20 linkage groups of soybean, in order to provide new soybean germplasm as well as molecular markers used in soybean breeding for resistance to SCN. The results suggested that Hartwig and Jin 1261, Jin 1265 or Jin 1267 had different resistance genes to SCN 4, with genetic similarity of 0.362. Twenty-two SSR markers from 11 linkage groups were associated with resistance to SCN 4, and at Satt684, Sat_230, Sat_222, Satt615 and Satt231 loci, the allele from Hartwig was resistant type, while the allele from Jin 1261, Jin 1265 and Jin 1267 was susceptible one, on the contrary at the other 17 SSR loci, the allele from Hartwig was susceptible type, while the allele from Jin 1261, Jin 1265 and Jin 1267 was resistant one. We supposed that Jin strains had at least a new resistance gene on linkage group D1b. We could effectively identify the resistance of these soybean lines to SCN 4 with Satt684 and Sat_400.

Key words: Soybean, Soybean lines, Soybean cyst nematode, Resistance, SSR