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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 203-210.doi: 10.3724/SP.J.1006.2009.00203

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect of HMW and LMW Glutenin Subunits on Processing Quality in Common Wheat

LEI Zhen-Sheng1,2;LIU Li2,3**;WANG Mei-Fang1,YAN Jun2,YANG Pan1;ZHANG Yan2;HE Zhong-Hu2,4,*   

  1. 1Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002; 2 National Wheat Improvement Center/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3 Crop Research Institute, Yunnan of Agricultural Sciences, Kunming 650205;4 CIMMYT-China Office, c/o Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2008-05-14 Revised:2008-07-17 Online:2009-02-12 Published:2008-12-10
  • Contact: HE Zhong-Hu

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Abstract:

Chinese wheat (Triticum aestivum L.) is generally considered to have acceptable protein content, but weak gluten properties, and is thus of inferior quality for mechanized production of pan bread and noodles in China. Therefore, improvement of gluten quality including strength and extensibility has become a major breeding objective. Composition of high molecular weight glutenin subunits (HMW-GS) and low molecular weight glutenin subunits (LMW-GS) play an important role in determinating the gluten quality in common wheat. The purposes of this study were to investigate the effects of allelic variation in HMW-GS and LMW-GS especially in subunit 14+15 and Glu-D3 locus on quality traits with two independent trials using 242 and 91 lines of progenies from the cross between PH 82-2 (Glutenin composition of 1, 14+15, 2+12, and Glu-A3d, Glu-B3d, Glu-D3c)and Neixiang 188 (Glutenin composition of 1, 7+9, 5+10, and Glu-A3a, Glu-B3j, Glu-D3b), respectively. The results showed that allelic variation had slight contribution to kernel protein content. Glu-D1 locus took a primary role in processing quality, Glu-D3 locus had slight contribution to them. The effect of locus was 14+15<7+9 at Glu-B1 and Glu-D3c>Glu-D3b at Glu-D3. 1B/1R translocation lines showed more desirable quality characteristics in peak time, right of peak slope, and peak integral than normal lines.

Key words: Common wheat, HMW-GS, LMW-GS, Processing quality

[1]Sugiyama T, Rafalski A, Peterson D, Soll D. A wheat HMW glu-tenin subunit gene reveals a highly repeated structure. Nucl Acids Res, 1985, 13: 8729-8737
[2]Payne P I. Genetics of wheat storage proteins and the effect of allelic variation of bread-making quality. Annu Rev Psychol, 1987, 38: 141-153
[3]Singh N K, Shpherd K W. Linkage mapping of the genes control-ling endosperm proteins in wheat: 1. Genes on the short arms of group 1 chromosomes. Theor Appl Genet, 1988, 75: 628-641
[4]Nagamine T, Kai Y, Takayama T, Yanagisawa T, Taya S. Allelic variation at the Glu-1 and Glu-3 loci in southern Japanese wheats, and its effects on gluten properties. J Cereal Sci, 2000, 32: 129-135
[5]Branlard G, Felix I. Part of the HMW glutenin subunits and omega gliadin allelic variants in the explanation of the quality parameters. In: Proceeding International Meeting—Wheat Kernel Proteins: Molecular and Functional Aspects, S. Martinal Cimino, Viterbo, Italy, 1994. pp 249-251
[6]Herbert W, Gerhard Z. Importance of amounts and proportions of high molecular weight subunits of glutenin for wheat quality. Eur Food Res Technol, 2000, 210: 324-330
[7]Kolster P, Vereiken J M. Evaluating HMW glutenin subunits to improve breadmaking quality of wheat. Cereal Foods World, 1993, 38: 76-82
[8]Huang D Y, Khan K. Quantitative determination of high molecu-lar weight glutenin subunit of hard red spring wheat by SDS-PAGE: I. Quantitive effects of total amounts on breadmak-ing quality characteristics. Cereal Chem, 1997, 74: 781-785
[9]Payne P I, Holt L M, Law C N. Structural and genetic studies on the high-molecular-weight subunits of wheat glutenin: I. Allelic variation in subunits amongst varieties of wheat (Triticum aesti-vum L.). Theor Appl Genet, 1981, 60: 229-236
[10]Branlard G, Dardevet M. Diversity of grain protein and bread wheat quality: II. Correlation between high molecular weight subunits of glutenin and flour quality characteristics. J Cereal Sci, 1985, 3: 345-354
[11]Gianibelli M C, Larroque O R, MacRichie F, Wrigley C W. Bio-chemical, genetic, and molecular characterization of wheat glu-tenin and its component subunits. Cereal Chem, 2001, 78: 635-646
[12]Carrillo J M, Rousset M, Qualset C O, Kasarda D D. Use of re-combinant inbred lines of wheat for studying the associations of high-molecular-weight glutenin subunits alleles to quantitative traits: 1. Grain yield and quality prediction tests. Theor Appl Genet, 1990, 79: 321-330
[13]Lagudah E S, O’Brien L, Halloran G M. Influence of gliadin composition and high molecular weight subunits of glutenin on dough properties in an F3 population of a bread wheat cross. J Cereal Sci, 1988, 7: 33-42
[14]Cornish G B, Panozzo J F, Wrigley C W. Victorian wheat protein families. Cereals 98. In: O’Brien L, Blakeney A B, Ross A S, Wrigley C W, eds. Proceeding 48th Australian Cereal Chemistry Conference. RACI: Melbourne, Australia, 1999. pp 183-188
[15]Bietz J A, Wall J S. Isolation and characterization of gliadin-like subunits from glutenin. Soil Biol Biochem, 1973, 50: 537-547
[16]Gupta R B, Paul J G, Cornish G B, Palmer G A, Bekes F, Rathjen A J. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheat: I. Its additive and interaction effects on dough properties. J Cereal Sci, 1994, 19: 9-17
[17]Luo C, Griffin W B, Brandlard G, McNeil D L. Comparison of low and high molecular weight wheat glutenin allele effects on flour quality. Theor Appl Genet, 2001, 102: 1088-1098
[18]Branlard G, Dardevet R, Saccomano F, Lagoutte F, Gourdon J. Genetics diversity of wheat storage proteins and bread wheat quality. Euphytica, 2001, 119: 59-67
[19]Gupta G B, MacRitchie F. A rapid one-step one-dimensional SDS-PAGE procedure for analysis of subunit composition of glu-tenin in wheat. J Cereal Sci, 1991, 14: 105-109
[20]Liu Y-H(刘艳华), Wang H-G(王洪刚), Liu S-B(刘树兵), Gao J-R(高居荣), Zhang H-L(张宏领). Study on the relationship be-tween 14+15, 7+8, 1 HMW glutenin subunits and some quality properties of wheat. Acta Agric Boreali-Sin (华北农学报), 2003, 18(3): 4-7(in Chinese with English abstract)
[21]Zhang H(张宏), Ren Z-L(任志龙), Ji W-Q(吉万全), Wang C-Y(王长有), Wang Q-Y(王秋英), Xue X-Z(薛秀庄), Cai D-M(蔡东明). The effects of pyramiding 14+15 and 5+10 sub-units on wheat quality in sister lines. Acta Bot Boreal-Occident Sin (西北植物学报), 2004, 24(12): 2272-2276(in Chinese with English abstract)
[22]Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Wang D-S(王德森), Zhang Y(张艳), Pe?a R J. Effect of allelic variation in HMW and LMW glutenin subunits on the processing quality in common wheat. Sci Agric Sin (中国农业科学), 2004, 37(1): 8-14(in Chi-nese with English abstract)
[23]Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Yan J(阎俊), Zhang Y(张艳), Pe?a R J. Effect of allelic variation at Glu-1 and Glu-3 loci on processing quality in common wheat. Acta Agron Sin (作物学报), 2004, 30(10): 959-968(in Chinese with English abstract)
[24]Liu L(刘丽), Zhou Y(周阳), He Z-H(何中虎), Pe?a R J, Zhang L-P(张立平). Effect of allelic variation at Glu-1 and Glu-3 loci on insoluble glutenin content. Acta Agron Sin (作物学报), 2004, 30(11): 1086-1092(in Chinese with English abstract)
[25]Liu L(刘丽), Zhou Y(周阳), Liu J-J(刘建军), He Z-H(何中虎), Yang J(杨金). Effect of allelic variation at the Glu-1 and Glu-3 loci and presence of 1BL/1RS translocation on pan bread and dry white Chinese noodle quality. Sci Agric Sin (中国农业科学), 2004, 37(9): 1265-1273(in Chinese with English abstract)
[26]Chen D-S(陈东升), Liu L(刘丽), Dong J-L(董建力), He Z-H(何中虎), Zhang Y(张艳), Liu J-J(刘建军), Wang D-S(王德森). Ef-fect of HMW and LMW glutenin subunits and presence of 1BL/1RS translocation on quality traits in spring wheat. Acta Agron Sin (作物学报), 2005, 31(4): 414-419(in Chinese with English abstract)
[27]Liu L, He Z H, Yan J, Zhang Y, Xia X C, Pe?a R J. Allelic varia-tion at the Glu-1 and Glu-3 loci, presence of 1B/1R translocation, and their effect on mixographic properties in Chinese bread wheats. Euphytica, 2005, 142: 197-204
[28]He Z H, Liu L, Xia X C, Liu J J, Pe?a R J. Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality of Chinese bread wheats. Cereal Chem, 2005, 82: 345-350
[29]He Z H, Yang J, Zhang Y, Quail K J, Pe?a R J. Pan bread and dry white Chinese noodle quality in Chinese winter wheats. Euphytica, 2004, 139: 257-267
[30]Pe?a R J, Amaya A, Rajaram S, Mujeep-Kazi A. Variation in quality of characteristics associated with some spring 1B/1R translocation wheats. J Cereal Sci, 1990, 12: 105-112
[31]Walker A E, Walker C E. Documentation and User Instructions for Mixsmart Software, Chapter 10. National Mfg: Lincoln, 1992
[32]Wang A L, Gao L Y, Li X H, Zhang Y Z, He Z H, Xia X C, Zhang Y, Yan Y M. Characterization of two 1D-encoded o-gliadin sub-units closely related to dough strength and pan bread-making quality in common wheat (Triticum aestivum L.). J Cereal Sci, 2008, 47: 528-535
[33]Martín P, Gomez M, Carrillo J M. Interaction between allelic variation at the Glu-D1 locus and 1BL/1RS translocation on flour quality in bread wheat. Crop Sci, 2001, 41: 1080-1084
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