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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 301-308.doi: 10.3724/SP.J.1006.2009.00301

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Quantitative Trait Loci for Alkaline Tolerance at Early Seedling Stage under Alkaline Stress in Japonica Rice

QI Dong-Ling1,2,GUO Gui-Zhen3,LEE Myung-Chul4,YANG Chun-Gang3,ZHANG Jun-Guo3,CAO Gui-Lan1,ZHANG San-Yuan3,SUH Seok-Cheol4,ZHOU Qing-Yang5,HAN Long-Zhi1,*   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Beijing 100081,China;2rubber Research Institute of China, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Physiology for Tropical Crops, Ministry of Agriculture, Danzhou 571737,China;3Institute of Rice Research,Jilin Academy of Agricultural Sciences, Gongzhuling 136100,China;4 National Institute of Agricultural biotechnology, Rural Development Administration, Suwon 441-707,Korea;5 Horticultural Department, Sichuan Agricultural University,Ya'an 625014,China
  • Received:2008-08-15 Revised:2008-10-05 Online:2009-02-12 Published:2008-12-11
  • Contact: HAN Long-Zhi

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Abstract:

The quantitative trait loci (QTLs) for root number, root length, seedling height and their relative alkaline damage rates at early seedling stage in rice under 0.15% Na2CO3 alkaline stress were identified using a F2:3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. All the traits above showed a continuous distribution with 1 or 2 peaks in F3 lines under alkaline stress, which were the quantitative traits controlled by major and multiple QTLs. Twenty-six QTLs associated with root number, root length and seedling height and their relative alkaline damage rates at early seedling stage under alkaline stress were detected, and which were located on chromosomes 1, 5, 6, 7, 8, 9, and 11. These QTLs included 4 QTLs associated with root number, 5 QTLs correlated with relative alkaline damage rate for root number, 6 QTLs controlling root length, 2 QTLs for relative alkaline damage rate for root length, 5 QTLs associated with seedling height, 4 QTLs related to relative alkaline damage rate for seedling height. qRN6-1, qRN11, qRRN11-2, qRRL11-2, qSH1, qSH11-2, qRSH5, and qRSH6-2, accouonted for 29.91, 13.42, 23.86, 21.06, 15.81, 16.53, 29.89, and 34.63% of the observed phenotypic variation, respectively. While the marker flanking distance of these QTLs explained larger. The observed phenotypic variation were more than 26.3 cM, except qRN6-1 with 19.0 cM of marker flanking distance, which should be conducted more refined mapping further. Of detected QTLs, the alleles for 13 QTLs originated from the tolerant parent Changbai 9, and the alleles of other QTLs were derived from alkaline sensitive parent Gaochan 106. These gene actions showed mainly part dominance or over dominant effects.

Key words: Rice, Alkaline tolerance, Early seedling stage, Microsatellite marker, Quantitative trait locus(QTL)

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