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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (4): 733-740.doi: 10.3724/SP.J.1006.2009.00733

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Physiological Response to 1,2,4-Trichlorobenzene Stress of Different Rice Genotypes in seedlings

ZHANG Guo-Liang12,CHEN Wen-Jun2,QIU Li-Min1,SUN Guo-Rong2,DAI Qi-Gen2,ZHANG Hong-Cheng2   

  1. 1College of Life Science and Chemistry Engineering,Huaiyin Institute of Technology,Huai'an 223001,China;2Key Laboratory for Genetics andPhysiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,China
  • Received:2008-06-18 Revised:2008-09-05 Online:2009-04-12 Published:2009-02-16
  • Contact: ZHANG Guo-Liang E-mail:hgzgl@sina.com

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Abstract:

1,2,4-Trichlorobenzene (TCB) has pervaded in industrial and agricultural production. Because of a series of problems due to its longevity and amassment, TCB has been added into the environmental pollutant list. In order to know how TCB affects the rice seed germination, seedling growth and its physiological characteristics, the seed germination, seedling biomass, soluble protein, and malnodialdehyde (MDA) contents, as well as activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in leaves and roots of seedlings treated with TCB were investigated in a sand culture experiment using rice cultivars, Xiangjing 20-18 (TCB tolerant genotype)and Siyang 1382(TCB sensitive genotype). The results indicated that TCB had little effect on the seed germination rate and index, however caused the significant reduction of seedling biomass, withXiangjing 20-18 being more affected than Siyang 1382. With TCB stress degree strengthening, soluble protein content of Xiangjing 20-18 leaves and roots presented anincreasing tendency, while that of Siyang 1382 leaves decreased significantly, and that of its roots decreased too under treatment of high TCB concentration. O2? producing velocity of leaves in two genotypes decreased firstly, then increased with increasing TCB concentration. That of Xiangjing 20-18 roots presented thesame tendency, and significantly higher than the control at TCB ≥0.6 mmol kg-1, while that of Siyang 1382 roots had no significant difference with that of the control. SOD activity of Xiangjing 20-18 leaves increased, and that of roots increased significantly. After TCB ≥0.2 mmol kg-1, SOD activity of Xiangjing 20-18 leaves was significantly higher than that of the control, that of Siyang 1382 leaves and roots was not significantly different with that of the control. POD activity of Xiangjing 20-18 leaves increased firstly, then decreased, and that of roots was significantly higher than the control, while that of Siyang 1382 leaves and roots decreased significantly. CAT activity of Xiangjing 20-18 leaves was higher or significantly higher than that of the control, and that of Siyang 1382 was contrary to the result of Xiangjing 20-18. MDA content of leaves decreased firstly, then increased, being significantly higher than that of the control at high TCB concentration, and that of roots increased significantly in two genotype. In conclusion, lower reduction of seedling biomass, high protein content of leaves and roots, the better active oxygen’s elimination ability and lower MDA content in plants would be considered as the physiological traits in TCB-tolerant genotypes of rice.

Key words: 1,2,4-trichlorbenzene, Rice, Tolerant genotype, Sensitive genotype, Toxicity, Antioxidant enzymes

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