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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1258-1264.doi: 10.3724/SP.J.1006.2010.01258

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Screening of Rice Resources against Rice Black-Streaked Dwarf Virus and Mapping of Resistant QTL

WANG Bao-Xiang1,JIANG Ling1**,CHEN Liang-Ming1,LU Bai-Guan2,WANG Qi1,LI Guang-Quan1,FAN Ji-Wei2,CHENG Xia-Nian1,ZHAI Hu-Qu3,XU Da-Yong2,WAN Jian-Min13*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Lianyungang Agricultural Science of Xuhuai Area , Lianyungang 222006, China; 3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-03-04 Revised:2010-04-22 Online:2010-08-12 Published:2010-06-11

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Abstract: Rice black-streaked dwarf virus(RBSDV)disease become epidemic in Jiangsu and Zhejiang provinces. To screen resistant germplasms and discover new resistance genes/QTLs against RBSDV, we evaluated the resistance to RBSDV in 311 japonica cultivars in field test. The results showed that no cultivar was immune to RBSDV. The disease rate of 24 main japonica cultivars grown in Jiangsu province at present was 10.0%–29.0% and that of 71.5% cultivars in 287 japonicapopularized in Jiangsu beforewas between 10.0% and 30.0%. Furthermore, quantitative trait loci (QTL) analysis was conducted by using 162 recombinant inbred lines (RILs), which derived from a cross between Guichao 2, a susceptible indica variety, and Koshihikari, a japonica variety with resistance to RBSDV. RBSDV resistances were evaluated using natural infection methods by scoring the disease rating. One putative QTL (qRBSDV3) controlling RBSDV resistance was mapped between the marker RM7 and RM5748 on chromosome 3, which explained 17.1% of the total phenotypic variation with LOD score of 5.4. The positive resistant effect contributed from Koshihikari. Further analysis revealed that the lines harboring the alleles of qRBSDV3 exhibited significantly increased resistance to RBSDV. The results should be very useful for breeding new RBSDV-resistant cultivars by marker-assisted selection (MAS). cultivars

Key words: Rice, Rice black-streaked dwarf virus, Screening resistant germplasm, QTL mapping

[1]         Milne R G, Lovisolo O. Maize rough dwarf and related viruses. Adv Virus Res, 1977, 21: 267–341

[2]         Azuhata F, Uyeda I, Kimura I, Shikata E. Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. J Gen Virol, 1993, 74: 1227–1232

[3]         Li A-H(李爱宏), Dai Z-Y(戴正元), Ji H-J(季红娟), Zhang X-X(张小祥), Li Y-H(李育红), Pan C-H(潘存红), Zhang H-Y(张洪熙), Pan X-B(潘学彪). Preliminary analysis on resistance of rice black-streaked dwarf viral disease for germplasms with different gene-types. J Yangzhou Univ (扬州大学学报), 2008, 29(3): 73–77 (in Chinese with English abstract)

[4]         Li D-B(李德葆), Wang G-C(王拱辰), Sheng F-J(盛方镜). Epidemological study on rice virus disease and their control in Zhejiang province. Acta Phytopathol Sin (植物病理学报), 1979, 9(2): 73–87 (in Chinese with English abstract)

[5]         Heng M Z, Yang J, Chen J P, Adama M J. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Arch Virol, 2008, 153: 1893–1898

[6]         Zhang H M, Chen J P, Lei J L, Adama M J. Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black-steaked dwarf virus. Eur J Plant Pathol, 2001, 107: 563–567

[7]         Wang H D, Chen J P, Wang A G, Jiang X H, Adama M J. Studies on th epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemic in Zhejiang province, China. Plant Pathol, 2009, 58, 815–825

[8]         Pan C-H(潘存红), Li A-H(李爱宏), Chen Z-X(陈宗祥), Wu L-B(吴林波), Dai Z-Y(戴正元), Zhang H-X(张洪熙), Huang L-S(黄年生), Chen X-J(陈夕军), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Pan X-B(潘学彪). Detection of QTL for resistance to rice black-streaked dwarf viral disease. Acta Agron Sin (作物学报) 2009, 35(12): 2213–2217 (in Chinese with English abstract)

[9]         Beijing Agricultural University (北京农业大学). Plant Pathology of Agriculture(农业植物病理学). Beijing: Agriculture Press, 1982 (in Chinese)

[10]      Zhang H M, Chen J P, Adams M J. Molecular characterization of segments 1 to 6 of rice black-streaked dwarf virus from China provides the complete genome. Arch Virol, 2001, 146: 2331–2339

[11]      Zuhata F, Uyeda I, Kimura I. Close similarity between genome structures of rice black-streaked dwarf virus and maize rough dwarf virus. J Gen Virol, 1993, 74: 1227–1232

[12]      Sogai M, Uyeda I, Lee B C. Detection and assignment of proteins encoded by rice black streaked dwarf fiji virus S7, S8, S9 and S10. J Gen Virol, 1998, 79: 1487–1494

[13]      Marzachi C M, Boccardo G, Nuse D. Cloning of the maize rough dwarf virus genome: Molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains with a single genomic segment. Virology, 1991, 180: 518–526

[14]      Bai F W, Yan J, Qu Z C, Zhang H W, Xu J, Ye M M, Shen D L. Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black streaked dwarf virus (RBSDV). Virus Genes, 2002, 25: 201–206

[15]      Wang C-H(王朝辉), Zhou Y-J(周益军), Fan Y-J(范永坚). Detection of rice black-streaked dwarf Fifivirus by RT-PCR, dot-blot hybridization and SDS-PAGE. J Nanjing Agric Univ (南京农业大学学报), 2001, 24 (4): 24–28 (in Chinese with English abstract)

[16]      Wang Z H, Fang S G, Zhang Z Y, Han C G, Li D W, Yu J L. Development of an ID-ELISA for the detection of rice black-streaked dwarf virus in plants. J Virol Methods, 2006, 134 (1-2): 61-5

[17]      Zhou G-H(周国辉), Wen J-J(温锦君), Cai D-J(蔡德江), Li P(李鹏), Xu D-L(许东林), Zhang S-G(张曙光). Chin Sci Bull (科学通报), 2007, 53 (20): 2500–2508 (in Chinese with English abstract)

[18]      Basten C J, Weir B S, Zeng Z B. QTL Cartographer, Version 1.16. 2002. Department of Statistics, North Carolina State University, Raleigh, NC. http://www.statgen.ncsu.edu/qtlcart

[19]      McCouch S R, Cho Y G, Yano M, Paule E, Blinstrue M, Mor-ishima H M, Kinosita T. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13

[20]      Hibino H. Biology and epidemiology of rice viruses. Annu Rev Phytopathol, 1996, 34: 249–274

[21]      Zhou T(周彤), Fan Y-J(范永坚), Chen Z-B(程兆榜), Zhou Y-J(周益军). Advances on resistance to rice stripe disease in rice cultivars. J Plant Genet Resour (植物遗传资源学报), 2009, 10(2): 328–333 (in Chinese with English abstract)

[22]      Sun D-Z(孙黛珍), Jiang L(江玲), Zhang Y-X(张迎信), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Wan J-M(万建民). Detection of QTL Associated with Rice Stripe Resistance in Cultivar IR24. Acta Agron Sin (作物学报), 2007, 33(1): 25–30 (in Chinese with English abstract)

[23]      Ding X-L(丁秀兰), Jiang L(江玲), Zhang Y-X(张迎信), Sun D-Z(孙黛珍), Zhai H-Q(翟虎渠), Wan J-M(万建民). QTL detection for rice stripe disease resistance using backcross inbred lines. Acta Agron Sin (作物学报), 2005, 31(8): 1041–1046 (in Chinese with English abstract)
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