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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 935-946.doi: 10.3724/SP.J.1006.2012.00935

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Association Analysis of Agronomic Traits and Resistance to Aspergillus flavus in the ICRISAT Peanut Mini-Core Collection

HUANG Li**,REN Xiao-Ping**,ZHANG Xiao-Jie,CHEN Yu-Ning,JIANG Hui-Fang*   

  1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2011-11-01 Revised:2012-02-22 Online:2012-06-12 Published:2012-04-06
  • Contact: 姜慧芳, E-mail: peanutlab@oilcrops.cn

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Abstract: Yield is an important trait in peanut breeding, which is strongly influenced by environments and complex genetic factors. Association mapping, based on natural population and linkage disequilibrium (LD), has been successfully used for exploring the genetic basis of complex traits in crops. In this study, we introduced a set of peanut mini-core collection of 146 varieties from International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), which were phenotyped for agronomic traits and resistance to Aspergillus flavus and genotyped by using 26 SSR primers containing 120 loci in the population. Based on the analyses of linkage disequilibrium, population structure and kinship, we performed the genome-wide association mapping. Distribution of LD suggested that the average of the total R2 was 0.185, indicating a relative low level of LD between the SSR loci. One hundred sixty-four varieties were grouped into two subgroups by population structure analysis corresponding to Arachis hypogaea ssp. fastigiata and Arachis hypogaea spp. hypogaea, respectively, which was consistent with botanical classification and results of analysis of genetic differentiation. A total of 39 loci were identified to be associated with ten agronomic traits, with 1.50%~20.34% of phenotypic variation explained. A total of 16 loci were associated with Aspergillus flavus infection index and aflatoxin amount, with 5.23%~17.19% of explained phenotypic variation. Of which, 13 common loci were associated with both agronomic traits and resistance to Aspergillus flavus. Furthermore, 63 alleles with increasing effect, 47 alleles with decreasing effect and the varieties carrying them were identified for ten agronomic and two resistance-related traits. This study demonstrates that association mapping is effective to explore genetic basis of important traits in peanut and assist to peanut breeding.

Key words: Arachis hypogaea L., Mini-core collection, SSR marker, Association analysis

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