Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 988-995.doi: 10.3724/SP.J.1006.2012.00988

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Fine Mapping of Drought Tolerance QTL on Chromosome 2 in Rice

NIE Yuan-Yuan1,2,ZOU Gui-Hua3,LI Yao4,LIU Guo-Lan2,CAI Yao-Hui1,MAO Ling-Hua1,YAN Long-An1,LIU Hong-Yan2,*,LUO Li-Jun2,*   

  1. 1 Rice Research Institute, Jiangxi Academy of Agricultural Sciences / Nanchang Branch of Chinese National Center for Rice Improvement, Nanchang 330200, China; 2 Shanghai Agriobiological Gene Center, Shanghai 201106, China; 3 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 4 Institute of Soil & Fertilizer and Resource & Environment, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
  • Received:2011-09-02 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 刘鸿艳, E-mail: lhy@sagc.org.cn, Tel: 021-62202915; 罗利军, E-mail: lijun@sagc.org.cn, Tel: 021-62204090

PDF

1969

Cited

2

Abstract: In rice breeding research, drought tolerance (DT) is one of the most important target traits for variety improvement under ever-increasing severe drought situation in the whole world. Identification of the physiological character and grain yield QTLs directly related to DT will provide useful marker information for developing with DT rice variety via marker-assisted selection (MAS). The 87 introgression lines (ILs) selected from 269 advanced backcross introgression population derived from Zhenshan97B/IRAT109 in the Zhenshan97B background were planted in drought facilities for mapping QTLs affecting physiological characters and grain yield under irrigation and drought stress conditions. Twenty QTLs affecting leaf water potential (LWP), canopy temperature (CT), basal culm thickness (BCT), 100-grain weight (HGW), spikelets number per panicle (SN) and spikelets density were identified, which could be grouped into three types based on their behaviors. Type I included seven QTLs which were detected under both conditions; type II consisted of four QTLs which were mapped only in the control condition; and type III consisted of two QTLs which were induced by drought and detected only under the stress. The seven QTLs detected under both environments and the two QTLs detected under drought stress could directly contribute to DT and be used in rice breeding for DT by MAS.

Key words: Rice, Near-isogenic lines (NIL), Drought tolerance (DT), Water relative physiological, Grain yield

[1]Brown L R, Halweil B. China’s water shortage could shake world food security. World Watch, 1998, 7: 3–4

[2]Luo L-J(罗利军), Zhang Q-F(张启发). The status and strategy on drought resistance of rice (Oryza sativa L.). Chin J Rice Sci (中国水稻科学), 2001, 15(3): 209–214 (in Chinese with English abstract)

[3]Teng S(腾胜), Qian Q(钱前), Zeng D-L(曾大力), Kunihiro Y(国广泰史), Fujimoto K(藤本宽), Huang D-N(黄大年), Zhu L-H(朱立煌). Analysis of gene loci and epistasis for drought tolerance in seedling stage of rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2002, 29(3): 235–240 (in Chinese with English abstract)

[4]Champoux M C, Wang G, Sarkarung S. Locating genes associated with root morphology and drought avoidance in rice via linkage to molecular markers. Theor Appl Genet, 1995, 90: 969–981

[5]Price A H, Tomos A D. Genetic dissection of root growth in rice (Oryza sativa L.): II. Mapping quantitative trait loci using molecular markers. Theor Appl Genet, 1997, 95: 143–152

[6]Ray J D, Yu L X, Mccouch S R. Mapping quantitative trait loci associated with root penetration ability in rice (Oryza sativa L.). Theor Appl Genet, 1996, 92: 627–636

[7]Xu J-C(徐吉臣), Li J-Z(李晶昭), Zheng X-W(郑先武), Zou L-X(邹亮星), Zhu L-H(朱立煌). QTL mapping of the root traits in rice seedling. Acta Genet Sin (遗传学报), 2001, 28(5): 433–438 (in Chinese with English abstract)

[8]Zheng B S, Yang L, Zhang W P. Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations. Theor Appl Genet, 2003, 107: 1505–1515

[9]Luo L-J(罗利军), Mei H-W(梅捍卫), Yu X-Q(余新桥), Liu H-Y(刘鸿艳), Feng F-J(冯芳君). Water-saving and drought-resistance rice and its development strategy. Chin Sci Bull (科学通报), 2011, 56(11): 804–811 (in Chinese with English abstract)

[10]Lafitte H R, Price A H, Courtois B. Yield response to water deficit in an upland rice mapping population: associations among traits and genetic markers. Theor Appl Genet, 2004, 109: 1237–1246

[11]Reynolds M P, Trethowan R M, Ginkel M, Rajaram S. General considerations in physiological breeding. In: Reynolds M P, Ortiz-Monasterio J I, McNab A, eds. Application of Physiology in Wheat Breeding. Mexico, International Maize and Wheat Improvement Center (CIMMYT), 2001. pp 2–86

[12]Liu H-Y(刘鸿艳), Zou G-H(邹桂花), Liu G-L(刘国兰), Hu S-P(胡颂平), Li M-S(李明寿), Yu X-Q(余新桥), Mei H-W(梅捍卫), Luo L-J(罗利军). Correlation analysis and QTL identification for canopy temperature, leaf water potential and spikelet fertility in rice under contrasting moisture regimes. Chin Sci Bull (科学通报) , 2005, 50(2): 130–139 (in Chinese with English abstract)

[13]Zou G H, Mei H W, Liu H Y, Liu G L, Hu S P, Yu X Q, Li M S, Wu J H, Luo L J. Grain yield responses to moisture regimes in a rice population: association among traits and genetic markers. Theor Appl Genet, 2005, 112: 106–113

[14]Garrity D P, O’Toole J C. Selection for reproductive stage drought avoidance in rice, using infrared thermometry. Agron J, 1995, 87: 773–779

[15]Wang D L, Zhu J, Li Z K, Paterson A H. Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. Theor Appl Genet, 1999, 99: 1255–1264

[16]Zeng H, Luo L, Zhang W, Zhou J, Li Z, Liu H, Zhu T, Feng X, Zhong Y. Plant QTL-GE: a database system for identifying candidate genes in rice and Arabidopsis by gene expression and QTL information. Nucl Acids Res, 2007, 35: D879–D882

[17]Bohnert H, Shen J. Transformation and compatible solutes. Sci Hort, 1998, 78: 237–260

[18]Hemamalini G S, Shashidhar H E, Hittalmani S. Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice (Oryza sativa L.). Euphytica, 2000, 112: 69–78

[19]Yue B, Xue W Y, Xiong L Z, Yu X Q, Luo L J, Cui K H, Jin D M, Xing Y Z, Zhang Q F. Genetic basis of drought resistance at reproductive stage in rice: separation of drought tolerance from drought avoidance. Genetics, 2006, 172: 1213–1228

[20]Nguyen T T T, Klueva N, Chamareck V, Aarti A, Magpantay G, Millena A C M, Pathan M, Nguyen H T. Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Gen Genet, 2004, 272: 35–46

[21]Lanceras J C, Pantuwan G, Jongdee B, Toojinda T. Quantitative trait Loci associated with drought tolerance at reproductive stage in rice. Plant Physiol, 2004, 35: 384–399

[22]Zhang J, Zheng H G, Aarti A, Pantuwan G, Nguyen T T, Tripathy J N, Sarial A K, Robin S, Babu R C, Nguyen B D, Sarkarung S, Blum A, Nguyen H T. Locating genomic regions associated with components of drought resistance in rice: comparative mapping within and across species. Theor Appl Genet, 2001, 103: 19–29

[23]Moncada P, Martinez C P, Borrero J, Chatel M, Gauch H, Guimaraes E, Tohme J, McCouch S R. Quantitative trait loci for yield and yield components in an Oryza sativa ? Oryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet, 2001, 102: 41–42

[24]Lafitte R, Blum A, Atlin G. Using secondary traits to help identify drought-tolerant genotypes. In: Fischer K S, Lafitte R, Fukai S, Atlin G, Hardy B, eds. Breeding Rice for Drought-Prone Environments. Los Banos, the Philippines: IRRI, 2003. pp 37–48

[25]Hemamalini G S, Shashidhar H E, Hittalmani S. Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice (Oryza sativa L.). Euphytica, 2000, 112: 69–78
[1] Ma Sheng-Qian, Wang Zhi-Ping, Chen Hao-Tian, Dou Shu-Xian, Zhang Yan, Deng Ai-Xing, Zhang Wei-Jian, Yuan Xiang-Yang, Song Zhen-Wei. Effects of tillage methods and nitrogen application rate on maize yield and soil aggregates in northeastern China under straw returning [J]. Acta Agronomica Sinica, 2026, 52(6): 1802-1816.
[2] Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[3] Zou Yi-Mei, Xu Min, Wang Hai-Yang, Yao Hui, Wang Jia-Feng, Liu Hao, Ren Dai-Sheng. Analysis of transcription factor regulatory networks in two-line male sterile rice seedling roots in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(6): 1728-1742.
[4] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[5] Yan An, Jiang Kun-Wei, Wang Rong-Yuan, Tian Lin, Zhang Lu, Wang Yun, Xu Jian-Long. Identification and cloning of SVN7 controlling small vascular bundle number in the rice flag leaf [J]. Acta Agronomica Sinica, 2026, 52(5): 1364-1372.
[6] Chen Wei, Wei Wan-Juan, Zhao Qi-Bing, Chang Dong-Wei, Yu Ling-Bo, Zhai Peng-Fei, Feng Zhi-Ming, Chen Zong-Xiang, Ren Yang-Tao, Yang Peng, Liu Hai-Lang, Li Zhen-Fu, Yang Yong-Le, Jin Yan-Gang, Zuo Shi-Min. Developing new germplasm of high-quality and early-maturing rice by editing Hd6 via CRISPR/Cas9 [J]. Acta Agronomica Sinica, 2026, 52(4): 1046-1056.
[7] Shi Shao-Jie, Liu Kai, Chen Zi-Yi, Wang Hui-Ying, Li San-He, Zhou Lei, You Ai-Qing. Cloning and functional analysis of the dwarf and multi-tiller gene DMT1 in rice [J]. Acta Agronomica Sinica, 2026, 52(4): 1022-1034.
[8] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[9] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[10] Ye Fan, Li Shuai, Li Si-Yu, Chen Yun, Dou Chao-Yin, Liu Li-Jun. Effects of water-saving irrigation on rice yield and population quality in Northeast China [J]. Acta Agronomica Sinica, 2026, 52(3): 895-907.
[11] Liu Ning, Fan Ping, Wang Cheng, Chen Qi-Qi, Cheng Qing-Yue, Tie Xia-Na, Tang Jing-Sha, Liu Bin-Bin, Xie Hong-Kun, Wang Jia-Yue, Shi Yuan-Qing, Ma Jun. Effects of reduced nitrogen application combined with organic fertilizer on yield formation and nitrogen utilization in mechanically transplanted rice [J]. Acta Agronomica Sinica, 2026, 52(3): 866-880.
[12] Zhu Jin-Juan, Wang Hui-Ping, Yang Guo-Dong, Wang Yu-Cheng, Yang Chen, Wang Bin, Agustiani Nurwulan, Tu Jun-Ming, Bi Jun-Guo, Cui Ke-Hui, Huang Jian-Liang, Peng Shao-Bing, Yuan Shen. Effects of water management and variety type on grain yield and quality in ratoon rice [J]. Acta Agronomica Sinica, 2026, 52(1): 295-315.
[13] WANG Chan, WU Ying-Ying, LI Wen-Qi, LI Xia, WANG Fang-Quan, ZHOU Tong, YANG Jie. Development of functional markers of rice stripe disease resistance gene STV11 based on HRM technique [J]. Acta Agronomica Sinica, 2025, 51(9): 2547-2556.
[14] GUO Bao-Wei, WANG Wang, WANG Kai, WANG Yan, ZENG Xin, JING Xiu, WANG Jing, NI Xin-Hua, XU Ke, ZHANG Hong-Cheng. Population dynamic characteristics and formation mechanisms of super high-yielding of two types of glutinous rice in the middle and lower reaches of the Yangtze Rive [J]. Acta Agronomica Sinica, 2025, 51(9): 2433-2453.
[15] YANG Ting-Ting, CHEN Juan, ABDUL Rehman, LI Jing, YAN Su-Hui, WANG Jian-Lai, LI Wen-Yang. Effects of weak light post-anthesis on dry matter accumulation and translocation, grain yield, and starch quality in soft wheat [J]. Acta Agronomica Sinica, 2025, 51(8): 2204-2219.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd