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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 1960-1968.doi: 10.3724/SP.J.1006.2012.01960

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Validation of CAPS Markers for Marker-Assisted Selection of Rice Blast Resistance Gene Pi25

WANG Hui-Mei1,**,CHEN Jie1,**,SHI Yong-Feng1,PAN Gang2,SHEN Hai-Chao1,WU Jian-Li1,*   

  1. 1 State Key Laboratory of Rice Biology / China National Rice Research Institute, Hangzhou 310006, China; 2 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
  • Received:2012-04-17 Revised:2012-07-05 Online:2012-11-12 Published:2012-09-10
  • Contact: WU Jian-Li; E-mail: beishangd@163.com; Tel: +86-571-63370326 **These authors contributed equally to this work.
  • Supported by:

    This work was supported by the National High Technology Research and Development Program of China (Grant No. 2011AA10A101 and 2012AA101102) and the Ministry of Finance, China (Grant No. 2012RG002-4).

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Abstract:

To promote the application of rice blast resistance gene Pi25 in rice breeding programs, we developed four sets of gene-specific CAPS markers (CAP1/Hinc II, CAP3/Bgl II, CAP3/Nde I, and CAP3/Hpy 99I) based on the coding sequences of the locus. One hundred and sixty-nine rice accessions, 98 recombinant inbred lines (RILs) and 217 transgenic plants were used for the validation of the markers. The results showed that all the four sets of markers were able to accurately and efficiently detect the Pi25/pi25 locus, CAP1/Hinc II and CAP3/Hpy 99I could digest specifically the dominant allele Pi25 while CAP3/Bgl I and CAP3/Nde I were able to digest specifically the recessive allele pi25. RILs and transgenic lines carrying Pi25 allele were resistant to the blast isolate JS001-20 while the lines carrying pi25 allele were susceptible, indicating a perfect detection of the target locus by the CAPS markers. In addition, a low frequency (1.2%) of the dominant allele was detected in the germplasm collections, indicating this gene has not been fully utilized in rice breeding programs in China. Markers CAP1/Hinc II and CAP3/Hpy 99I are recommended and will be useful for the improvement of blast resistance, especially for the early-season indica rice.

Key words: Oryza sativa, Blast resistance, Cleaved amplified polymorphic sequence (CAPS), Marker-assisted selection (MAS), Single nucleotide polymorphism (SNP)

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