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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2312-2319.doi: 10.3724/SP.J.1006.2012.02312

• RESEARCH NOTES • Previous Articles    

Genetic Diversity in 30 Tobacco Varieties Analyzed by IMP Markers

XIE He1,GAO Yu-Long1,SONG Zhong-Bang1,GUI Yi-Jie2,FAN Long-Jiang2,XIAO Bing-Guang1,LU Xiu-Ping1,*   

  1. 1 Yunnan Academy of Tobacco Agriculture Science, Yuxi 653100, China; 2 College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2012-03-20 Revised:2012-08-15 Online:2012-12-12 Published:2012-10-08
  • Contact: 卢秀萍, E-mail: xplu@yntsti.com, Tel: 0877-2075055

Abstract:

Miniature Inverted repeat Transposable Element (MITEs) is a high copy number transposon family in plants. Molecular markers based on closely located MITEs are termed Inter MITE polymorphisms or IMP. We conducted the genetic distance evaluation and cluster analyses of 30 tobacco cultivars using IMP markers. Among 133 primers screened, 32 primers had steady polymorphism bands. A total of 185 polymorphic bands were detected out of 30 cultivars, with 5.78 polymorphism bands per IMP primer. The genetic distance among cultivars ranged from 0.02 to 0.81, with an average of 0.33. According to cluster analyses, 30 tobacco cultivars could be classified into three groups. Group I included sun-cured tobacco, oriental tobacco and flue-cured tobacco of northern China; group II included USA NC series, Coker series and China Yunyan series; group III included two burley tobacco cultivars. The cluster analysis result closely matched the classic relationship among the cultivars. The result showed that the IMP markers, successfully amplified in tobacco, generated fairly polymorphism and could be applied in genetic diversity analysis. Thus, IMP markers establish a foundation for Tobacco genetic study and marker-assisted selection. The genetic diversity is very low among 30 tobacco cultivars investigated in this study, indicating that a strategy to enrich tobacco germplasm for further breeding should be developed.

Key words: IMP molecular marker, Tobacco, Genetic diversity

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