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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (04): 626-631.doi: 10.3724/SP.J.1006.2013.00626

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification and Gene Mapping of Dwarf and Brittle Culm Mutant dbc1 in Oryza sativa

SANG Xian-Chun,DU Chuan,WANG Xiao-Wen,YANG Zheng-Lin,LING Ying-Hua,ZHAO Fang-Ming,LI Yun-Feng,HE Guang-Hua*   

  1. Rice Research Institute of Southwest University / Chongqing Key Laboratroy of Application and Safety Control of Genetically Modified Crops / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
  • Received:2012-09-03 Revised:2012-11-16 Online:2013-04-12 Published:2013-01-04
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

Abstract:

One dwarf and brittle culm mutant dbc1 was identified from the progeny of restorer line Jinhui10 which seeds were treated by ethyl methanesulfonate (EMS). From the seedling stage to the mature stage, dbc1 showed obvious characteristics of dwarfism and frangibility. In detail, the mutantshortened each internode with a plant height of 58.93 cm, belonging to the dn type of dwarfism mutation. Treated by GA3, the dbc1 showed more insensitive compared with its original parent Jinhui10. One thousand grain weight, seed-setting rate, and tiller numbers did not change while panicle length, grain number per panicle and full grain number decreased significantly in the mutant. Further analysis showed that mechanical strengths declined significantly in the mutational leaf blades and culms. Interestingly, cellulose content was not changed and lignin content slightly decreased compared with those of wild type, with a significant difference at P<0.05 between them. Genetic analysis indicated that the mutation was controlled by one recessive nuclear gene. In this paper, DBC1 gene was finally mapped between SSR markers RM13943 and RM13952 on chromosome 2 based on 886 F2 mutational plants of Xinong1A/dbc1. In the restricted region, the physical distance was 197 kb and contained 52 annotated genes. These results provide a foundation for DBC1 gene cloning and its application in the molecular breeding.

Key words: Rice (Oryza sativa), Dwarf, Brittle culms, Gibberellins-insensitive, Gene mapping

[1]Hu J(胡江), Zeng D-L(曾大力), Zhang G-H(张光恒), Guo L-B(郭龙彪), Dong G-J(董国军), Gao Z-Y(高振宇), Hu X-M(胡兴明), Zhu L(朱丽), Liu J(刘坚), Qian Q(钱前). Genetic analysis and gene mapping of a new mutant of dwarf and disproportionate uppermost-internodel (ddul) in rice. Chin J Rice Sci (中国水稻科学), 2009, 23 (3): 252–256 (in Chinese with English abstract)



[2]Liang G H, Cao X Y, Sui J M, Zhao X Q, Yan C J, Yi C D, Gu M H. Fine mapping of a semidwarf gene sd-g in indica rice (Oryza sativa L.). Chin Sci Bull, 2004, 49(9): 900–904



[3]Zhang F-T(张帆涛), Fang J(方军), Sun C-H(孙昌辉), Li R-B(李润宝), Luo X-D(罗向东), Xie J-K(谢建坤), Deng X-J(邓晓建), Chu C-C(储成才). Characterization of a rice dwarf and twist leaf 1 (dtl1) mutant and fine mapping of DTL1 gene. Hereditas (Beijing) (遗传), 2012, 34(1): 79–86 (in Chinese with English abstract)



[4]Wei C-X(韦存虚), Xie P-S(谢佩松), Zhou W-D(周卫东), Chen Y-F(陈义芳), Yan C-J(严长杰). Anatomical structure and chemical features of leaf in brittle mutant of rice. Acta Agron Sin (作物学报), 2008, 34(8): 1417–1423 (in Chinese with English abstract)



[5]Li Y, Qian Q, Zhou Y, Yan M, Sun L, Zhang M, Fu Z, Wang Y, Han B, Pang X, Chen M. BRITTLE CULM 1, which encodes a COBRA-like protein, affects the mechanical properties of rice plants. Plant Cell, 2003, 15: 2020–2031



[6]Updegraff D M. Semimicro determination of cellulose in biological materials. Anal Biochem, 1969, 32: 420–424



[7]Musel G, Schindler T, Bergfeld R, Ruel K, Jacquet G, Lapierre C, Speth V, Schopfer P. Structure and distribution of lignin in primary and secondary cell walls of maize coleoptiles analyzed by chemical and immunological probes. Planta, 1997, 201: 146–159



[8]Lichtenthaler H K. Hlorophylls and carotenoids pigments of photosynthetic biomenbranes. Meth Enzymol, 1987, 48: 350–382



[9]Michelmore R M, Papan I, Kesseli R V. Identification of marks linked to disease resistance genes by bulked segregant analysis: A rapid method to detect marks in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[10]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (Beijing) (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)



[11]Davies P J, ed. Plant Hormones: Physiology, Biochemistry and Molecular Biology. The Netherlands: Kluwer Academic Publishers, 1995. pp 13–38



[12]Sakamoto T, Miura K, Itoh H, Tatsumi T, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Agrawal G, Takeda S, Abe K, Miyao A, Hirochika H, Kitano H, Ashikari M, Matsuoka M. An overview of gibberellin metabolism enzyme genes and their related mutant in rice. Plant Physiol, 2004, 134: 1642–1653



[13]Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Minobe Y. Positional cloning of rice semi-dwarfing gene, sd-1: rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res, 2002, 9: 11–17



[14]Itoh H, Ueguchi-Tanaka M, Sentoku N, Kitano H, Mtsuoka M. Cloning and functional analysis of two gibberellin 3B- hydroxylase genes that are differently expressed during the growth of rice. Proc Nat1 Acad Sci USA, 2001, 98(15): 8909–89014



[15]Itoh H, Tatsumi T, Sakamoto T, Otomo K, Toyomasu H, Ashikari M, Ichihara S, Matsuoka M. A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase. Plant Mol Biol, 2004, 54: 533–547



[16]Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Ghow T, C. Hising Y, Kitano H, Yamaguchi I, Matsuoka M. Gibberellin insensitive Dwarf1 encodes a soluble receptor for gibberellin. Nature, 2005, 437: 693–698



[17]Itoh H, Ueguchi Tanaka M, Sato Y, Ashikri M, Matsuoka M. The gibberellin signaling pathway is regulated by the appearance and disappearance of SL ENDER rice in nuclei. Plant cell, 2002, 14: 57–70



[18]Ashikari M, Itoh H, Ueguchi-Tanaka M, Akie S, Kenji G, Hidemi K, Makoto M. Gibberellin signal transduction in rice. J Plant Growth Regul, 2003, 22: 141–151



[19]Ikeda A, Ueguchi Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J. Slender rice, a constitution gibberellin response mutant, is caused by a null mutation on of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. Plant Cell, 2001, 13: 999–1010



[20]Gao Z-Y(高振宇), Liu X-H(刘晓辉), Guo L-B (郭龙彪), Liu J (刘坚), Dong G-J(董国军), Hu J(胡江), Han B(韩斌), Qian Q(钱前). Identification of a novel tillering dwarf mutant and fine mapping of the tddl(t) gene in rice (Oryza sativa L.). Chin Sci Bull (科学通报), 2009, 54(9): 1238–1243 (in Chinese)



[21]Wang F(王峰), Tang Y-Q(唐彦强), Miao R-L(苗润隆), Xu F-F(徐芳芳), Lin T-T(林婷婷), He G-H(何光华), Sang X-C(桑贤春). Identification and gene mapping of a narrow and upper-albino leaf mutant in rice (Oryza sativa L.). Chin Sci Bull (科学通报), 2012, 57(22): 2066–2071 (in Chinese)



[22]Crowell E F, Gonneau M, Stierhof Y D, Höfte H, Vernhettes S. Regulated trafficking of cellulose synthases. Curr Opin Plant Biol, 2010, 13: 700–705



[23]Zhang B C, Zhou Y H. Rice brittleness mutants: A Way to open the ‘BlackBox’ of monocot cell wall biosynthesis. J Integr Plant Biol, 2011, 53 (2): 136–142



[24]Wu B, Zhang B, Dai Y, Zhang L, Shang-Guan K, Peng Y, Zhou Y, Zhu Z. Brittle Culm15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant Physiol, 2012, 159(4): 1440–1452



[25]Zhang B C, Liu X L, Qian Q, Liu L F, Dong G J, Xiong G Y, Zeng D L, Zhou Y H. Golgi nucleotide sugar transporter modulates cell wall biosynthesis and plant growth in rice. Proc Natl Acad Sci USA, 2011, 108(12): 5110–5115



[26]Zhou Y H, Li S B, Qian Q, Zeng D L, Zhang M, Guo L B, Liu X L, Zhang B C, Deng L W, Liu X F, Luo G Z, Wang X J, Li J Y. BC10, a DUF266-containing and Golgi-located type II membrane protein, is required for cell-wall biosynthesis in rice (Oryza sativa L.). Plant J, 2009, 57: 446–462



[27]Wang C-L(王川丽), Wang L-Q(王令强), Mou T-M(牟同敏). Characterization and gene mapping of a brittle culm mutant nbc1(t) in rice. J Huazhong Agric Univ (华中农业大学学报), 2012, 31(2): 159–164 (in Chinese with English abstract)



[28]Zhang S-J(张水金), Zheng Y(郑轶), Zhu Y-S(朱永生), Yang D(杨东), Tu S-H(涂诗航), Zhou P(周鹏), Zheng J-T(郑家团), Huang T-X(黄庭旭). Advance in research on brittleness mutant of rice. Fujian J Agric Sci (福建农业学报), 2011, 26(5): 895–898 (in Chinese with English abstract)

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