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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (06): 951-964.doi: 10.3724/SP.J.1006.2014.00951

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Genetic Similarity and Specificity of Ten Important Soybean Cultivar Families Released in China

XIONG Dong-Jin1,2,**,WANG Wu-Bin1,**,ZHAO Tuan-Jie1,GAI Jun-Yi1,*   

  1. 1 Soybean Research Institute of Nanjing Agricultural University / National Center for Soybean Improvement / National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China; 2 School of Life Science and food Engineering, Nanchang University, Nanchang 330031, China
  • Received:2013-11-03 Revised:2014-03-04 Online:2014-06-12 Published:2014-04-09
  • Contact: 盖钧镒, E-mail: sri@njau.edu.cn E-mail:sri@njau.edu.cn

Abstract:

Analysis of the affinity relationship and genetic similarity among cultivars is important for crop genetic improvement. In this study, a total of 161 SSRs covering the entire soybean genome were analyzed for the genetic diversity, similarity and specificity of 10 important families composed of 179 cultivars using PowerMarker Ver. 3.25. The results showed that there were totally 1697 alleles, averaging 10.5 per locus, ranging from 5 to 24 with average polymorphism information content of 0.832, ranging from 0.545 to 0.943 in the population. According to the SSR cluster analysis, the 179 cultivars were clustered into six groups, eleven subgroups, with a tendency that the cultivars in a family tended to be grouped into a same cluster. There existed significant correlation (r = 0.67) between coefficient of parentage (CP) and genetic similarity coefficient (GSC) of the population. The CP and GSC values of A295, A133, A122, and A231 families were relatively low, which means that the genetic bases of the four families were relatively broad. In contrast, the CP and GSC values of A291, A201, A084, and A002 families were relatively high, indicating their genetic bases were relatively narrow due to more cultivars obtained from pure line selection. The CP and GSC values between the A019 family from Northeast China and other nine families were the lowest among all pairwise combinations of families. The genetic specificity analysis showed that there existed much more complementary alleles, specifically existent and specifically deficient alleles in A019 in comparison with in other families, indicating the former is distant from the latter. On the other hand, the families in Eco-region II and III, contained fewer complementary alleles, specifically existent and specifically deficient alleles, which might be due to some frequent germplasm exchange between the neighboring eco-regions. For example, there were no specifically existent alleles in A002, A231, A122 and no specifically deficient alleles in A084, A201, A034, and A231. The present results are of significance in broadening the genetic basis of soybean cultivar.

Key words: Soybean, Released cultivar, SSR, Genetic diversity, Specificity

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