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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (08): 1380-1385.doi: 10.3724/SP.J.1006.2014.01380

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of a Gene Causing Hybrid Pollen Sterility between Yujing 6B and TR2604

ZHANG Hong-Gen**,SUN Yi-Biao**,FENG Zhi-Qiang,QIAN Kai,PEI Yan,LI Chuang,TANG Shu-Zhu*,LIANG Guo-Hua,GU Ming-Hong   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2013-12-27 Revised:2014-04-16 Online:2014-08-12 Published:2014-05-16
  • Contact: 汤述翥, E-mail: sztang@yzu.edu.cn **同等贡献(Contributed equally to this work)

Abstract:

Understanding the genetic basis of spikelet fertility differences between japonica hybrids is good for BT-type three-line japonica hybrid breeding. According to the pollen and spikelet fertility of the plants in the populations derived from Yujing 6A(B), 9201A(B) and TR2604, it was confirmed that the sterility of Yujing 6A(B)/TR2604 F1 plants was induced by the incompatibility between the parents. Genetic analysis suggested that the hybrid pollen sterility character was controlled by a single nuclear gene named S38(t). A total of 352 plants from Yujing 6A//TR2604/TR2604 and Yujing 6B/TR2604//Yujing 6B segregation populations were used for gene mapping, and S38(t) wasmapped between RM18 and RM234 on chromosome 7 with genetic distances of 0.43 cM and 0.14 cM, respectively. The physical distance between RM18 and RM234 was about 118 kb. These results are useful for the map-based cloning of S38(t) gene.

Key words: Three-line japonica hybrid, Hybrid pollen sterility, Genetic analysis, Gene mapping

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