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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (06): 832-843.doi: 10.3724/SP.J.1006.2016.00832

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of bZIP Protein Family in Gossypium arboreum and Tissue Expression Analysis of GaFDs Genes

ZHANG Yan-Nan,CAI Da-Run,HUANG Xian-Zhong*   

  1. Plant Genomics Laboratory / College of Life Sciences, Shihezi University, Shihezi 832003, China
  • Received:2015-11-13 Revised:2016-01-11 Online:2016-06-12 Published:2016-03-14
  • Contact: 黄先忠, E-mail: xianzhongh106@163.com, Tel: 0993-2057262 E-mail:zhangyn0513@163.com
  • Supported by:

    This study was supported by the Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-12-1072), National Natural Science Foundation of China (31360366), and Program for Doctor Foundation in XinJiang Production and Construction Corps (2012BB007).

Abstract:

The basic leucine zipper (bZIP) is one of the largest and most diverse transcription factors in eukaryotes, and is involved in various processes of plant growth and development and in response to biotic and abiotic stresses. In this study, 159 bZIP family genes were identified and their complete gene sequences were obtained by using bioinformatics analysis method, based on Gossypium arboreumwhole genome database, and 159 bZIP genes were loaded on 13 chromosomes. The 159 bZIP genes were categorized into 13 groups based on their phylogenetic relationships, gene structures and conserved motifs. In addition, three GaFD homologous genes GaFD1, GaFD2 and GaFD3 were identified, which belongs to A subfamily in G. arboretum. The expression patterns of GaFD genes in different tissues were determined by using quantitative Real-time reverse transcription PCR (qRT-PCR) method. The results showed that GaFD1 and GhFD2 were preferentially expressed in the shoot apical meristem (SAM), whereas GaFD3 was preferentially expressed in stem. The results revealed that a number of bZIP family members exist in cotton genome, and FD genes with different structures and expression patterns play different roles in the development of cotton, which provides valuable information for dissecting the function and mechanism of bZIPs in cotton.

Key words: Basic leucine zipper, Gossypium arboreum, FD, Phylogenetic analysis, Gene expression

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