Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (08): 1215-1223.doi: 10.3724/SP.J.1006.2016.01215

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Plant Growth Regulators on Key Enzymes in Sucrose Metabolism of Ear Leaf and Grain at Filling Stage of Spring Maize

LI Guang-Yan,WANG Qing-Yan,XU Yan-Li,LU Lin,Jiao Liu,DONG Xue-Rui,DONG Zhi-Qiang   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Ecophysiology and Cultivation, Beijing 100081, China
  • Received:2015-12-31 Revised:2016-05-09 Online:2016-08-12 Published:2016-05-30
  • Contact: 董志强, E-mail: dongzhiqiang@caas.cn, Tel: 010-82106043 E-mail:liguangyan5112@126.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31470087), the National Key Technology R&D Program of China (2012BAD04B01-07, 2012BAD4B02) and Science and Technology InnovationProject of Chinese Academy of Agricultural Sciences

Abstract:

In order to explore the new cultivation techniques, chemical control was established inthis study.We conducted a field experiment using two varieties of Zhongdan 909 and Jidan 35with treatments of leaf-sprayingethylene-chlormequat-potassium (ECK) in V6, andPASP-KT-NAA (PKN) in V9. The results showed that ECK & PKN increased the activities of sucrose synthetase(SS)and sucrose phosphate synthetase (SPS) and sucrose content during early grain filling stage (10-20 d after anthesis)in ear leaf, and decreased sucrose content in ear leaf during late grain filling stages (30-50 d after anthesis),especiallyit improved the ability of sucrose synthesis at the late filling stage (50 d after anthesis). ECK & PKN also decreased SS (synthesis) activity in grain during middle and late grain filling stages and increased SS (resolving) activity, promoting sucrose decomposition ability, withless effect on SS(synthesis) and SPS activitiesin grain. The yields of Zhongdan 909 and Jidan 35 compared with their own control were respectively increased by 4.61% and 7.78%.The studyindicated that ECK & PKN enhance sucrose synthesis in leaf and decompositionability in grain, andpromote the translocation of carbon assimilation to grain, resulting in highgrain-filling rate,high grain weight, and high yield.

Key words: Spring maize, ECK, PKN, Sucrose, Key enzymes of sucrose metabolism

[1]夏叔芳, 于新建, 张振清. 叶片光合产物输出的抑制与淀粉和蔗糖的积累. 植物生理学报, 1981,7:136–141
Xia S F, Yu J X, Zhang Z Q. Inhibition of photosynthetic product output and accumulation of starch and sucrose in leaves. J Plant Physiol, 1981,7:136–141(in Chinese)
[2]张明方, 李志凌. 高等植物中与蔗糖代谢相关的酶. 植物生理学报, 2002, 38:289–295
Zhang M F, Li Z L. Sucrose-metabolizing enzymes in higher plants. J Plant Physiol,2002, 38:289–295(in Chinese)
[3]Jang J C, Sheen J. Sugar sensing in higher plants.Plant Cell,1997, 6:1665–1679
[4]赵福成, 景立权, 闫发宝, 陆大雷, 王桂跃, 陆卫平. 灌浆期高温胁迫对甜玉米籽粒糖分积累和蔗糖代谢相关酶活性的影响. 作物学报,2013, 39: 1644–1651
Zhao F C, Jing L Q, Yan B F, Lu D L, Wang G Y, Lu W P. Effects of heat stress during grain filling on sugar accumulation and enzyme activity associated with sucrose metabolism in sweet corn.ActaAgron Sin,2013, 39: 1644–1651 (in Chinese with English abstract)
[5]覃鸿妮, 蔡一林, 孙海燕, 王久光, 王国强, 刘志斋. 种植密度对不同株型玉米蔗糖代谢和淀粉合成相关酶活性的影响.中国生态农业学报, 2010, 18: 1183–1188
Qin H N, Cai Y L, Sun HY, Wang J G, Wang GQ, Liu ZZ. Effects of planting density on sucrose metabolism and activities of enzymesrelated to starch synthesis in maize hybrids with different planttypes. Chin J Eco-Agric, 2010, 18: 1183–1188 (in Chinese with English abstract)
[6]赵宏伟, 孔宇,于秋竹, 杨亮. 密度对饲用玉米蔗糖合成和积累的影响.玉米科学, 2009, 17(5): 72–76
Zhao H W, Kong Y, Yu Q Z, Yang L. Effects of density on sucrose synthesize and accumulation of forage maize. JMaize Sci, 2009, 17(5): 72–76 (in Chinese with English abstract)
[7]王俊忠, 黄高宝, 张超男, 杨亚军, 赵会杰, 朱晓燕, 马培芳. 施氮量对不同肥力水平下夏玉米碳氮代谢及氮素利用率的影响.生态学报,2009, 29: 2045–2052
Wang J Z, Huang G B, Zhang C N, Yang Y J, Zhao H J, Zhu X Y, Ma P F. Influence of nitrogen fertilizer rate on carbon-nitrogen metabolism and nitrogen use efficiency of summer maize under high and medium yield levels. ActaEcol Sin,2009, 29: 2045–2052 (in Chinese with English abstract)
[8]曹玉军, 赵宏伟, 王晓慧, 魏雯雯, 张磊, 王立春, 王永军. 施钾对甜玉米产量、品质及蔗糖代谢的影响. 植物营养与肥料学报, 2011,29: 881–887
Cao Y J, Zhao H W, Wang X H, Wei W W, Zhang L, Wang L C, Wang Y J. Effects of potassium fertilization on yield, quality and sucrose metabolism of sweet maize. PlantNutrFertSci,2011,29: 881–887 (in Chinese with English abstract)
[9]王庆燕, 管大海, 潘海波, 李建民, 段留生, 张明才, 李召虎. 油菜素内酯对春玉米灌浆期叶片光合功能与产量的调控效应.作物学报, 2015, 41: 1557–1563
Wang Q Y, Guan D H, Pan H B, Li J M, Duan L S, Zhang M C, Li Z H. Effect of brassinolide on leaf photosynthetic function and yield in springmaize filling stage.ActaAgron Sin, 2015, 41: 1557–1563(in Chinese with English abstract)
[10]刘鹏, 胡昌浩, 董树亭, 王空军, 张吉旺, 张保仁. 甜质型与普通型玉米籽粒发育过程中糖代谢相关酶活性的比较.中国农业科学, 2005, 38: 52–58
Liu P, Hu C H, Dong S T, Wang K J, Zhang J W, Zhang B R. Comparison of enzymes activity associated with sucrose metabolism in the developing grains between sweet corn and normal corn. SciAgric Sin,2005, 38: 52–58 (in Chinese with English abstract)
[11]董学会, 李建民, 何钟佩, 段留生, 李召虎. 30%己乙水剂对玉米叶片光合酶活性与同化物分配的影响.玉米科学,2006, 14(4): 93–96
Dong X H, Li J M, He Z P, Duan L S, Li Z H. Effects of 30% Hex-ethyl-aqua on photosynthetic enzyme activities and as simulation distribution in maize plants.JMaize Sci, 2006, 14(4): 93–96 (in Chinese with English abstract)
[12]卢霖, 董志强,董学瑞, 焦浏, 李光彦, 高娇. 乙矮合剂对不同密度夏玉米花粒期叶片氮素同化与早衰的影响.作物学报, 2015, 41: 1870–1879
Lu L, Dong Z Q, Dong X R, Jiao L, Li G Y, Gao J. Effects of Ethylene-Chlormequat-Potassium on leaf nitrogen assimilationafter anthesis and early senescence under different planting densities. ActaAgron Sin, 2015, 41: 1870–1879 (in Chinese with English abstract)
[13]徐田军, 董志强, 高娇, 陈传晓, 焦浏, 解振兴. 聚糠萘水剂对不同积温带玉米叶片衰老和籽粒灌浆速率的影响. 作物学报, 2012, 38: 1698–1709
Xu T J, Dong Z Q, Gao J, Chen C X, Jiao L, Xie Z X. Effect of PASP-KT-NAA on leaf senescence and grain filling rate during the grain-filling period in different temperature zones.ActaAgron Sin, 2012, 38: 1698–1709 (in Chinese with English abstract)
[14]高娇, 董志强, 徐田军, 陈传晓, 焦浏, 卢霖, 董学瑞. 聚糠萘水剂对不同积温带玉米花后叶片氮同化的影响. 生态学报, 2014, 34: 2938–2947
Gao J, Dong Z Q, Xu T J, Chen C X, Jiao L, Lu L, Dong X R.Effects of PASP-KT-NAA on maize leaf nitrogen assimilation after florescence over different temperature gradients.ActaEcol Sin, 2014, 34: 2938–2947 (in Chinese with English abstract)
[15]陈传晓, 董志强, 高娇, 徐田军, 焦浏, 卢霖, 张凤路. 聚糠萘水剂对不同积温带春玉米灌浆期光合性能的影响.玉米科学, 2013, 21(3): 66–70
Chen C X, Dong Z Q, Gao J, Xu T J, Jiao L, Lu L, Zhang F L. Effects of PASP-KT-NAA on the photosynthetic performances of different maize cultivars in different accumulated temperature zones. JMaize Sci, 2013, 21(3): 66–70 (in Chinese with English abstract)
[16]赵智中, 张上隆, 徐昌杰,陈昆松, 刘拴桃. 蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用. 园艺学报, 2001, 28:112–118
Zhao Z Z, Zhang S L, Xu C J, Chen K S, Liu S T. The role of sucrose metabolizing enzymes in accumulation of sugars in Satsuma mandarin.ActaHortic Sin, 2001, 28:112–118 (in Chinese)
[17]陈传永, 王荣焕, 赵久然, 徐田军, 王元东, 刘秀芝, 刘春阁, 裴志超, 成广雷, 陈国平. 不同生育时期遮光对玉米籽粒灌浆特性及产量的影响. 作物学报, 2014, 40: 1650–1657
Chen C Y, Wang R H, Zhao J R, Xu T J, Wang Y D, Liu X Z, Liu C G, Pei Z C, Cheng G L, Chen G P.Effects of shading on grain-filling properties and yield of maize at differentgrowth stages. ActaAgron Sin, 2014, 40: 1650–1657 (in Chinese with English abstract)
[18]Gottwald J R, Krysan P J, Young J C, Evert R F, Sussman M R. Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters.Proc Natl AcadSci USA,2000,97: 13979–13984
[19]Farrar J, Pollock C, Gallagher J. Sucrose and the integration of metabolism in vascular plants.Plant Sci,2000, 154: 1–11
[20]陈传晓. 不同积温带春玉米碳代谢机理及化学调控效应的研究. 河北农业大学硕士学位论文, 河北石家庄, 2013
Chen C X. Studies on the Mechanism of Carbon Metabolism of Spring Maize and Chemical Regulation Effects under Different Accumulated Temperature Zones. PhDDissertation of Agricultural University of Hebei, Shijiazhuang, China, 2013 (in Chinese with English abstract)
[21]王文静. 不同穗型冬小麦籽粒灌浆期源库强度及其与淀粉积累的关系. 作物学报, 2004, 30:916–921
Wang W J. The relationship between source-sink intensity and starch accumulation during grain filling period in two winter wheat cultivars with different spike types.ActaAgron Sin, 2004, 30:916–921 (in Chinese with English abstract)
[22]Eschrich W, Eschrich B. Control of phloem unloading by source activities and light. Plant PhysiolBiochem, 1992, 25: 625–634
[23]Dickinson C D, Altabella T, Chrispeels M J. Slow-growth phenotype of transgenic tomoto expressing apoplasticinvertase. Plant Physiol, 1991, 95: 420–425
[24]Weber H, Borisjuk L, Wobus U. Sugar import and metabolism during seed development. Trends Plant Sci, 1997, 2: 169–174
[25]Cheng WH, Chourey PS. Sucrose phosphate synthase expression at the cell and tissue level is coordinated with sucrose sink-to-source transitions in maize leaf. Plant Physiol,1996,111: 1021–1029
[26]张海艳, 董树亭, 高荣岐, 李玉全. 玉米籽粒淀粉积累及相关酶活性分析.中国农业科学, 2008, 41: 2174–2181
Zhang H Y, Dong S T, Gao R Q, Li Y Q. Starch accumulation and enzymes activities associated with starch synthesis in maize kernels.SciAgric Sin, 2008, 41: 2174–2181(in Chinese with English abstract)
[27]Hubbard N L, Huber S C, Pharr D M. Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing Muskmelon (Cucumis melo L.) fruits. Plant Physiol, 1989, 91:1527–1534
[28]张超男, 赵会杰, 王俊忠,付晓记. 不同施肥方式对夏玉米碳水化合物代谢关键酶活性的影响. 植物营养与肥料学报, 2008, 14:54-58
Zhang C N, Zhao H J, Wang J Z, Fu X J. Effect of different fertilization methods on activities of key enzymes in carbohydrate metabolism of summer maize.PlantNutrFertSci, 2008, 14:54–58 (in Chinese with English abstract)
[29]陈洋, 赵宏伟. 氮素用量对春玉米穗位叶蔗糖合成关键酶活性的影响. 玉米科学, 2008, 16(1): 115–118
Chen Y, Zhao H W. Effect of nitrogen application on activities of key enzymes of sucrose synthesis in the leaf located near the ear of spring maize.JMaize Sci, 2008, 16(1): 115–118 (in Chinese with English abstract
[30]李合生. 现代植物生理学(第2版). 北京: 高等教育出版社, 2006. pp 207–208
Li H S. Modern Plant Physiology,2ndedn. Beijing: Higher Education Press, 2006. pp 207–208 (in Chinese)
[31]Borkovec V, Prochazka S. Pre-anthesis interaction of cytokinins and ABA in the transport of 14C-sucrose to the ear of winter wheat (TriticumaestivumL.).J Agron Crop Sci, 1992,169: 229–235

[1] SONG Tian-Xiao, LIU Yi, RAO Li-Ping, Soviguidi Deka Reine Judesse, ZHU Guo-Peng, YANG Xin-Sun. Identification and expression analysis of cell wall invertase IbCWIN gene family members in sweet potato [J]. Acta Agronomica Sinica, 2021, 47(7): 1297-1308.
[2] GAO Zhen, LIANG Xiao-Gui, ZHANG Li, ZHAO Xue, DU Xiong, CUI Yan-Hong, ZHOU Shun-Li. Effects of irrigating at different growth stages on kernel number of spring maize in the North China Plain [J]. Acta Agronomica Sinica, 2021, 47(7): 1324-1331.
[3] ZHENG Ying-Xia, CHEN Du, WEI Peng-Cheng, LU Ping, YANG Jin-Yue, LUO Shang-Ke, YE Kai-Mei, SONG Bi. Effects of planting density on lodging resistance and grain yield of spring maize stalks in Guizhou province [J]. Acta Agronomica Sinica, 2021, 47(4): 738-751.
[4] TANG Jing-Quan, WANG Nan, GAO Jie, LIU Ting-Ting, WEN Jing, YI Bin, TU Jin-Xing, FU Ting-Dong, SHEN Jin-Xiong. Bioinformatics analysis of SnRK gene family and its relation with seed oil content of Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(3): 416-426.
[5] ZHU Ya-Li, WANG Chen-Guang, YANG Mei, ZHENG Xue-Hui, ZHAO Cheng-Feng, ZHANG Ren-He. Response of grain filling and dehydration characteristics of kernels located in different ear positions in the different maturity maize hybrids to plant density [J]. Acta Agronomica Sinica, 2021, 47(3): 507-519.
[6] LEI Yong, WANG Zhi-Hui, HUAI Dong-Xin, GAO Hua-Yuan, YAN Li-Ying, LI Jian-Guo, LI Wei-Tao, CHEN Yu-Ning, KANG Yan-Ping, LIU Hai-Long, WANG Xin, XUE Xiao-Meng, JIANG Hui-Fang, LIAO Bo-Shou. Development and application of a near infrared spectroscopy model for predicting high sucrose content of peanut seed [J]. Acta Agronomica Sinica, 2021, 47(2): 332-341.
[7] LI Wei-Tao, GUO Jian-Bin, YU Bo-Lun, XU Si-Liang, CHEN Hai-Wen, WU Bei, GONG Ting-Feng, HUANG Li, LUO Huai-Yong, CHEN Yu-Ning, ZHOU Xiao-Jing, LIU Nian, CHEN Wei-Gang, JIANG Hui-Fang. Establishment of HPLC-RID method for the determination of soluble sugars in peanut seed [J]. Acta Agronomica Sinica, 2021, 47(2): 368-375.
[8] LIU Peng-Zhao,SHI Zu-Jiao,NING Fang,WANG Rui,WANG Xiao-Li,LI Jun. Critical nitrogen dilution curves and nitrogen nutrition diagnosis of spring maize under different precipitation patterns in Weibei dryland [J]. Acta Agronomica Sinica, 2020, 46(8): 1225-1237.
[9] LI Rui-Jie,TANG Hui-Hui,WANG Qing-Yan,XU Yan-Li,WANG Qi,LU Lin,YAN Peng,DONG Zhi-Qiang,ZHANG Feng-Lu. Effects of 5-aminolevulinic acid and ethephon compound on carbon balance of source-sink of spring maize in Northeast China [J]. Acta Agronomica Sinica, 2020, 46(7): 1063-1075.
[10] WANG Dan-Dan, LIU Hong-Juan, WANG Hong-Xia, ZHANG Peng, SHI Chun-Yu. Cloning and functional analysis of the sweet potato sucrose transporter IbSUT3 [J]. Acta Agronomica Sinica, 2020, 46(7): 1120-1127.
[11] ZHANG Yu-Qin,YANG Heng-Shan,LI Cong-Feng,ZHAO Ming,LUO Fang,ZHANG Rui-Fu. Effects of strip-till with staggered planting on yield formation and shoot-root characteristics of spring maize in irrigation area of Xiliaohe plain [J]. Acta Agronomica Sinica, 2020, 46(6): 902-913.
[12] Wei BAI,Zhan-Xiang SUN,Li-Zhen ZHANG,Jia-Ming ZHENG,Liang-Shan FENG,Qian CAI,Wu-Yan XIANG,Chen FENG,Zhe ZHANG. Effects of plough layer construction on soil three phase rate and root morphology of spring maize in northeast China [J]. Acta Agronomica Sinica, 2020, 46(5): 759-771.
[13] Fang NING,Yuan-Hong ZHANG,Peng-Fei WEN,Rui WANG,Qian WANG,Zhao-Yang DONG,Guang-Can JIA,Jun LI. Responses of maize growth and yield to nitrogen application in dryland under different precipitation conditions [J]. Acta Agronomica Sinica, 2019, 45(5): 777-791.
[14] Hui-Hui TANG,Yan-Li XU,Qing-Yan WANG,Zheng-Bo MA,Guang-Yan LI,Hui DONG,Zhi-Qiang DONG. Increasing spring maize yield by basic application of PASP chelating nitrogen fertilizer in northeast China [J]. Acta Agronomica Sinica, 2019, 45(3): 431-442.
[15] Hai-Chao FAN,Wan-Rong GU,De-Guang YANG,Ju-Ping YU,Lin PIAO,Qian ZHANG,Li-Guo ZHANG,Xiu-Hong YANG,Shi WEI. Effect of Chemical Regulators on Physical and Chemical Properties and Lodging Resistance of Spring Maize Stem in Northeast China [J]. Acta Agronomica Sinica, 2018, 44(6): 909-919.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!