Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (05): 708-717.doi: 10.3724/SP.J.1006.2017.00708

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Function Analysis of Metal Tolerance Gene (CsMTP11) in Tea Plant (Camellia sinensis L. O. Kuntze)

YUAN Lian-Yu,CHEN Ying-Juan,WEI Xu,TONG Hua-Rong*   

  1. College of Food Science, Southwest University, Chongqing 400715, China
  • Received:2016-08-23 Revised:2017-01-21 Online:2017-05-12 Published:2017-02-17
  • Contact: Dong Huarong, E-mail: huart@swu.edu.cn, Tel: 023-68251298 E-mail:yuanlianyu88@163.com
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31400583), the Natural Science Foundation Project of CQ CSTC (cstc2014jcyjA80011), and Fundamental Research Funds for the Central Universities (XDJK2014C070).

Abstract:

As a member of cation diffusion facilitator family, metal tolerance protein (MTP) is involved in the regulation of heavy metal stress process in plant. Here, we cloned a MTP gene, CsMTP11 (GenBank accession number: KX450265) from Zhongcha 108 tea plant. The full-length cDNA of CsMTP11 is 1197 bp, and the gene encodes a novel protein of 398 amino acids, which shares significant sequence similarity with VvMTP11(90%). The molecular weight and theoretical pI of this protein are 44.85 kD and 5.34, respectively. The conserved domain analysis indicated that CsMTP11 contained five transmembrane domains and the conserved CDF domain. The phylogenetic tree indicated that CsMTP11 had sequence conservation among different species. The transcriptional level of CsMTP11 in old leaf was higher than that in root. Futher more, the heavy metal Mn and Co stresses induced the expression of CsMTP11. The subcellular localization assay using CsMTP11-YFP fusion gene expressed in the Arabidopsis protoplast showed that CsMTP11 was localized in the plasma membranes. CsMTP11 heterologous expression in wild-type yeast BY4741 and the mutants showed that it was able to increase tolerance to Mn and Co. Taken together , our results indicated that CsMTP11 is a member of the Mn-CDF family, and it may be involved in the regulation of heavy metal stress process in tea plant.

Key words: Camellia sinensis, Heavy metal tolerance, Gene clone, Mn

[1] Szymczycha-Madeja A, Welna M, Pohl P. Elemental analysis of teas and their infusions by spectrometric methods. Trac-Trends Anal Chem, 2012, 35: 165–181 [2] Brzezicha-Cirocka J, Grembecka M, Szefer P. Monitoring of essential and heavy metals in green tea from different geographical origins. Environ Monitor Assess, 2016, 188: 1–11 [3] Santos D, Batoreu C, Mateus L, Dos Santos A P M, Aschner M. Manganese in human parenteral nutrition: Considerations for toxicity and biomonitoring. Neurotoxicology, 2014, 43: 36–45 [4] Sloot W N, Gramsbergen J B P. Axonal-transport of manganese and its relevance to selective neurotoxicity in the rat basal ganglia. Brain Res, 1994, 657: 124–132 [5] 雷雨, 苏晓倩, 王芳, 李珍, 萧力争. 茶树重金属胁迫研究概况. 中国食物与营养, 2010, (5): 19–23 Lei Y, Su X Q, Wang F, Li Z, Xiao L Z. Research situation of heavy metal stress in tea plant. Food and Nutrition in China, 2010, (5): 19–23 [6] 常硕其, 彭克勤, 张亚莲, 刘红艳, 付海平. 加锰处理对茶树锰含量及生理变化的影响研究. 茶叶科学, 2008, (5): 331–338 Chang S Q, Peng K Q, Zhang Y L, Liu H Yan, Fu H P. Effect of Mn addition treatment on accumulation of Mn and physiological active substance in tea plants. Journal of Tea Science, 2008, (5): 331–338 [7] Ozdemir Y, Gucer S. Speciation of manganese in tea leaves and tea infusions. Anal Lett, 1998, 31: 679–689 [8] Yemane Michael, Chandravanshi B S, Wondimu Taddese. Levels of essential and non-essential metals in leaves of the tea plant (Camellia sinensis L.) and soil of Wushwush farms, Ethiopia. Food Chem, 2008, 107: 1236–1243 [9] Street R, Szakova J, Drabek O, Mladkova L. The status of micronutrients (Cu, Fe, Mn, Zn) in tea and tea infusions in selected samples imported to the Czech Republic. Czech J Food Sci, 2006, 24: 62–71 [10] 姚元涛, 张丽霞, 宋鲁彬, 田丽丽. 茶树锰素营养研究现状与展望. 中国茶叶, 2009, (3): 10–11 Yao Y T, Zhang L X, Song L B, Tian L L. Research status andprospect of manganese nutrition in tea plant. China Tea, 2009, (3): 10–11 [11] Memon A R, Chino M, Hidaka H, Hara K, Yatazawa M. Manganese toxicity in field-grown tea plants and the micro-distribution of manganese in the leaf tissues as revealed by electron-probe X-ray micrography. Soil Sci Plant Nutr, 1981, 27: 317–328 [12] 姚元涛, 陶吉寒, 宋鲁彬, 田丽丽, 刘腾飞, 贾厚振. 钙、锰、铝及与硼的协同胁迫对茶树的毒害效应. 植物生理学报, 2015, 11: 1867–1872 Yao Y T, Yao J H, Song L B, Tian L L, Liu T F, Jia H Z. Poison effects of synergistic stress of calcium, manganese, aluminum and boron on tea plant. Plant Physiol J, 2015, 11: 1867–1872 [13] Yuan L Y, Yang S G, Liu B X, Zhang M, Wu K Q. Molecular characterization of a rice metal tolerance protein, OsMTP1. Plant Cell Rep, 2012, 31: 67–79 [14] Lorraine W E, Pittman Jon K. Dissecting pathways involved in manganese homeostasis and stress in higher plant cells. In: Hell R, Mendel R R, Eds. Cell Biology of Metals and Nutrients. Berlin, Germany: Springer-Verlag Berlin, Heidelberger Platz 3, D-14197, 2010. 95–117 [15] Paulsen I T, Saier M H. A novel family of ubiquitous heavy metal ion transport proteins. J Membr Biol, 1997, 156: 99–103 [16] Wei Y N, Fu D. Binding and transport of metal ions at the dimer interface of the Escherichia coli metal transporter YiiP. J Biol Chem, 2006, 281: 23492–23502 [17] Montanini B, Blaudez D, Jeandroz S, Sanders D, Chalot M. Phylogenetic and functional analysis of the cation diffusion facilitator (CDF) family: improved signature and prediction of substrate specificity. BMC Genomics, 2007, 8: 107 [18] Becher M, Talke I N, Krall L, Kramer U. Cross-species microarray transcript profiling reveals high constitutive expression of metal homeostasis genes in shoots of the zinc hyperaccumulator Arabidopsis halleri. Plant J, 2004, 37: 251–268 [19] Cho M, Chardonnens A N, Dietz K J. Differential heavy metal tolerance of Arabidopsis halleri and Arabidopsis thaliana: a leaf slice test. New Phytol, 2003, 158: 287–293 [20] Migocka M, Papierniak Anna, Maciaszczyk-Dziubinska E, Pozdzik P, Posyniak E, Garbiec A, Filleur S. Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana. J Exp Bot, 2014, 65: 5367–5384 [21] Chen Z H, Fujii Y M, Yamaji N, Masuda S, Takemoto Y, Kamiya T, Yusuyin Y, Iwasaki K, Kato S I, Maeshima M, Ma J F, Ueno D. Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family. J Exp Bot, 2013, 64: 4375–4387 [22] Delhaize E, Gruber B D, Pittman J K, White R G, Leung H, Miao Y S, Jiang L W, Ryan P R, Richardson A E. A role for the AtMTP11 gene of Arabidopsis in manganese transport and tolerance. Plant J, 2007, 51: 198–210 [23] Tanaka N, Fujiwara T, Tomioka R, Kraemer U, Kawachi M, Maeshima M. Characterization of the histidine-rich loop of arabidopsis vacuolar membrane zinc transporter AtMTP1 as a sensor of zinc level in the cytosol. Plant Cell Physiol, 2015, 56: 510–519 [24] Arrivault S, Senger T, Kramer U. The Arabidopsis metal tolerance protein AtMTP3 maintains metal homeostasis by mediating Zn exclusion from the shoot under Fe deficiency and Zn oversupply. Plant J, 2006, 46: 861–879 [25] Gaitan-Solis E, Taylor N J, Siritunga D, Stevens W, Schachtman D P. Overexpression of the transporters AtZIP1 and AtMTP1 in cassava changes zinc accumulation and partitioning. Front Plant Sci, 2015, 6: 492 [26] Delhaize E, Gruber B D, Pittman J K, White R G, Leung H, Miao Y S, Jiang L W, Ryan P R, Richardson A E. A role for the AtMTP11 gene of Arabidopsis in manganese transport and tolerance. Plant J, 2007, 51: 198–210 [27] Delhaize E, Kataoka T, Hebb D M, White R G, Ryan P R. Genes encoding proteins of the cation diffusion facilitator family that confer manganese tolerance. Plant Cell, 2003, 15: 1131–1142 [28] Nies D H, Silver S. Ion efflux systems involved in bacterial metal resistances. J Ind Microbiol, 1995, 14: 186–199 [29] Kambe T, Narita H, Yamaguchi-Iwai Y, Hirose J, Amano T, Sugiura N, Sasaki R, Mori K, Iwanaga T, Nagao M. Cloning and characterization of a novel mammalian zinc transporter, zinc transporter 5, abundantly expressed in pancreatic beta cells. J Biol Chem, 2002, 277: 19049–19055 [30] Singh S, Parihar P, Singh R, Singh V. P, Prasad S. M. Heavy metal tolerance in plants: role of transcriptomics, proteomics, metabolomics, and lonomics. Front Plant Sci, 2016, 6: 1143-1178 [31] Kobae Y, Uemura T, Sato M H, Ohnishi M, Mimura T, Nakagawa T, Maeshima M. Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol, 2004, 45: 1749–1758 [32] Desbrosses-Fonrouge A G, Voigt K, Schroder A, Arrivault S, Thomine S, Kramer U. Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation. Febs Lett, 2005, 579: 4165–4174

[1] Zhang Li-Lan, Yang Jun, Wang Rang-Jian. Identification of candidate genes related to glycoside aroma precursor content in tea plant using WGCNA [J]. Acta Agronomica Sinica, 2026, 52(2): 494-513.
[2] Yu Kai-Hang, Zhou Hong-Bin, Luo Liang-Zha, Wang Mei-Li, Jiang Rui-Mei, Dong-Chen Wen-Hua, Li Shi-Jin, Mao Xiao-Qiang, Chen Sheng-Wei. Cloning and expression analysis of the HvLRR-RLK-510 gene encoding a leucine-rich repeat receptor-like kinase in barley [J]. Acta Agronomica Sinica, 2026, 52(2): 421-432.
[3] AI Sha, LI Sha, FANG Zhi-Wei, LI Lun, LI Tian-Tian, GAO Li-Fen, CHEN Li-Hong, XIAO Hua-Feng, WAN Ren-Jing, YAN Duo-Zi, WU Xing-Ting, PENG Hai, HAN Rui-Xi, ZHOU Jun-Fei. Development and application of cotton MNP marker for fingerprint cons- truction [J]. Acta Agronomica Sinica, 2024, 50(9): 2267-2278.
[4] GUO Si-Yu, ZHAO Ke-Yong, DAI Zheng-Gang, ZOU Hua-Wen, WU Zhong-Yi, ZHANG Chun. Functional analysis of maize N-acetyltransferase ZmNAT1 gene in response to abiotic stress [J]. Acta Agronomica Sinica, 2024, 50(8): 2001-2013.
[5] ZHANG Li-Lan, YANG Jun, WANG Rang-Jian. Genome-wide association study and candidate gene prediction of nerolidol and linalool primeveroside content in tea plants [J]. Acta Agronomica Sinica, 2024, 50(4): 871-886.
[6] WANG Juan, XU Xiang-Bo, ZHANG Mao-Lin, LIU Tie-Shan, XU Qian, DONG Rui, LIU Chun-Xiao, GUAN Hai-Ying, LIU Qiang, WANG Li-Ming, HE Chun-Mei. Characterization and genetic analysis of a new allelic mutant of Miniature1 gene in maize [J]. Acta Agronomica Sinica, 2023, 49(8): 2088-2096.
[7] ZHANG Lu-Lu, ZHANG Xue-Mei, MU Wen-Yan, HUANG Ning, GUO Zi-Kang, LUO Yi-Nuo, WEI Lei, SUN Li-Qian, WANG Xing-Shu, SHI Mei, WANG Zhao-Hui. Grain Mn concentration of wheat in main wheat production regions of China: Effects of cultivars and soil factors [J]. Acta Agronomica Sinica, 2023, 49(7): 1906-1918.
[8] WANG Rang-Jian, YANG Jun, ZHANG Li-Lan, GAO Xiang-Feng. Genome-wide association analysis of geraniol primrose glycoside abundance in tender tea shoots [J]. Acta Agronomica Sinica, 2023, 49(7): 1843-1859.
[9] JING Xiao-Jing, YANG Xin-Sun, JIN Xiao-Jie, LIU Yi, LEI Jian, WANG Lian-Jun, CHAI Sha-Sha, ZHANG Wen-Ying, JIAO Chun-Hai. Cloning and characterization of IbMAPKK9 gene associated with Fusarium oxysporum f. sp. batatas in sweet potato [J]. Acta Agronomica Sinica, 2023, 49(12): 3289-3301.
[10] DING Meng-Li, WANG Ru-Yin, SHI Dong-Sheng, LI Ying-Bo, LEI Jie, CHEN Hong-Yu, SHEN Qing-Wen, WANG Gui-Feng. Map-based cloning and transcriptomic analysis of a maize miniature kernel mutant mn-Mu [J]. Acta Agronomica Sinica, 2023, 49(11): 3122-3130.
[11] ZHANG Ling-Xiao,JIAO Zhen-Zhen,BU Hua-Hu,WANG Yi-Ru,LI Jian,ZHENG Jun. Functional identification of maize cation/proton antiporter ZmNHX7 [J]. Acta Agronomica Sinica, 2020, 46(8): 1185-1194.
[12] JIANG Hui-Bing,YANG Sheng-Mei,LIU Yu-Fei,TIAN Yi-Ping,SUN Yun-Nan,CHEN Lin-Bo,TANG Yi-Chun. Biological characteristics and cytological studies on anther abortion of male sterile Camellia crassocolumna [J]. Acta Agronomica Sinica, 2020, 46(7): 1076-1086.
[13] Tao FENG,Chun-Yun GUAN. Cloning and characterization of phytochrome interacting factor 4 (BnaPIF4) gene from Brassica napus L. [J]. Acta Agronomica Sinica, 2019, 45(2): 204-213.
[14] Yu-Zhang CHEN,Shou-Xi CHAI,Hong-Bo CHENG,Yu-Wei CHAI,Chang-Gang YANG,Kai-Min TAN,Lei CHANG. Effects of straw-incorporation combined with autumn plastic mulching on soil water consumption characteristics and winter wheat yield in arid farming areas [J]. Acta Agronomica Sinica, 2019, 45(2): 256-266.
[15] Tao FENG,Chun-Yun GUAN. Cloning and Characterization of Brassinazole-resistant (BnaBZR1 and BnaBES1) CDS from Brassica napus L. [J]. Acta Agronomica Sinica, 2018, 44(12): 1793-1801.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!