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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (02): 228-237.doi: 10.3724/SP.J.1006.2020.92032

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Influence of phosphorus on rice (Oryza sativa L.) grain zinc bioavailability and its relation to inositol phosphate profiles concentration

SU Da1,2,WU Liang-Quan2,K. Rasmussen Søren3,ZHOU Lu-Jian4,PAN Gang4,CHENG Fang-Min4,*()   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops / College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
    2 International Magnesium Institute, Fuzhou 350002, Fujian, China
    3 Department of Plant and Environmental Sciences, Section of Plant and Soil Science, University of Copenhagen, Copenhagen, Denmark
    4 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
  • Received:2019-06-03 Accepted:2019-08-09 Online:2020-02-12 Published:2019-09-10
  • Contact: Fang-Min CHENG E-mail:chengfm@zju.edu.cn
  • Supported by:
    This study was supported by the National Key Research and Development Project(2016YFD0300502);the National Natural Science Foundation of China(31571602);the Fujian Province Natural Science(2019J01374);the Education and Scientific Research Project for Middle-aged and the Young Teachers in Fujian Province(JAT170156);the Foundation for China Scholarship Council

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Abstract:

The hidden hunger caused by grain zinc (Zn) deficiency in crop poses a potential threat to the health of nearly two billion people worldwide, especially in developing countries. In this study, the long-term phosphorus fertilizer experiment and in vitro detached rice panicle culture systems with varied phosphorus levels were conducted to investigate the effect of phosphorus on rice grain Zn bioavailability and its relation of grain inositol phosphates profiles (phytic acid related metabolic derivatives) concentration. In our results, compared with low phosphorus level, high phosphorus supply increased grain phytic acid phosphorus and total phosphorus concentration (mg g -1). Moreover, high phosphorus supply also increased different grain inositol phosphate profile concentrations (InsP1-6), especially for InsP4-6. On the contrary, grain Zn concentration decreased with phosphorus supply. Both the decrement of Zn and increment of phytic acid phosphorus induced by the higher phosphorus supply in rice grain led to the significant decrement of grain Zn bioavailability. In in vitro detached panicle culture system, the Zn bioavailability in P12 treatment decreased by 81.3% relative to P0 treatment. In conclusion, higher phosphorus input could significantly decrease grain Zn bioavailability through increased grain phytic acid phosphorus and inositol phosphates derivatives concentration, in addition to the decrement of grain Zn concentration.

Key words: phosphorus, phytic acid, zinc bioavailability, inositol phosphate, rice quality

Fig. 1

Differences in PAP, Total P concentration (mg g-1), and PAP/total P among various phosphorus fertilizer levels in field experiment Bars indicated by the same letter among treatments are not significantly different at the 0.05 probability level. LP, MP, HP mean the low phosphorus level, medium phosphorus level, and high phosphorus level. PAP: phytic acid-phosphorus; Total P: total phosphorus; PAP/total P: phytic acid-phosphorus/total phosphorus."

Fig. 2

Effects of different phosphorus levels on grain Zn, Fe, Mn, and Cu concentrations and Zn bioavailability (TAZ) (field phosphorus fertilizer experiment) Bars indicated by the same letter among treatments are not significantly different at the 0.05 probability level. LP, MP, HP mean the low phosphorus level, medium phosphorus level, and high phosphorus level, respectively. TAZ represents Zn bioavailability."

Fig. 3

Effects of different phosphorus levels on grain PAP, total P concentration, and the ratio of PAP to total P (detached panicle culture experiment) Bars indicated by the same letter among treatments are not significant at the 0.05 probability level. P0, P1, P3, P6, P12 mean the P (NaH2PO4·2H2O ) levels of 0, 1, 3, 6, and 12 mmol L-1, respectively. PAP, total P, PAP/total P mean phytic acid-phosphorus, total phosphorus, and phytic acid-phosphorus/total phosphorus, respectively."

Fig. 4

Effect of exogenous phosphorus treatments on grain P distributions in brown rice in detached panicle culture experiment (scanning electron microscopic images of brown rice with 5000 ×; scale bars are 100 μm) A, B, C represent P0, P3, and P12 treatments, respectively."

Fig. 5

Effects of different phosphorus levels on grain inositol phosphate (InsP1-6) concentrations (detached panicle culture experiment) P0, P1, P3, P6, P12 mean the P (NaH2PO4·2H2O) levels of 0, 1, 3, 6, and 12 mmol L-1, respectively."

Fig. 6

Ratio of inositol phosphate composition/total inositol phosphate in different exogenous phosphorus treatment (detached panicle culture experiment) A: P0; B: P1; C: P3; D: P6; E: P12."

Fig. 7

Effect of exogenous phosphorus treatment on grain Zn distributions in detached panicle culture experiment (scanning electron microscopic images of brown rice with 5000 ×; scale bars are 100 μm) A, B, C represent P0, P3, and P12 treatment, respectively."

Fig. 8

Effects of different phosphorus levels on grain Zn concentration and its bioavailability (TAZ) (detached panicle culture experiment) Bars indicated by the same letter among treatments are not significant at the 0.05 probability level. P0, P1, P3, P6, P12 mean the P (NaH2PO4·2H2O) levels of 0, 1, 3, 6, and 12 mmol L-1, respectively. TAZ: Zn bio-availability."

Fig. 9

Correlation of grain phosphorus components and Zn bioavailability under P treatment (left: heatmap of field phosphorus fertilizer experiment; right: correlation in both field and detached rice panicle culture systems) PAP: phytic acid phosphours; TP: total phosphours; TAF: Zn bio-availabiity. ** P < 0.01."

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