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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (9): 1340-1350.doi: 10.3724/SP.J.1006.2020.91076

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Development and application of InDel markers based on high throughput sequencing in barley

XU Ting-Ting(), WANG Qiao-Ling, ZOU Shu-Qiong, DI Jia-Chun, YANG Xin, ZHU Yin, ZHAO Han, YAN Wei*()   

  1. Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences / Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm / Jiangsu Key Laboratory for Agrobiology, Nanjing 210014, Jiangsu, China
  • Received:2019-12-26 Accepted:2020-04-15 Online:2020-09-12 Published:2020-05-08
  • Contact: Wei YAN E-mail:13913024600@163.com;yanwei@jaas.ac.cn
  • Supported by:
    National Key Research and Development Program of China(2017YFD0101205);National Key Research and Development Program of China(2017YFD0102005);Jiangsu Agricultural Science and Technology Innovation Fund(CX(18)3001)

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Abstract:

Molecular markers are the basic tools in genetic research and widely used in genetic diversity analysis, germplasm identification, genetic map construction and gene mapping. Based on the whole genome re-sequencing data of barley, we developed 118 pairs of InDel markers covering the whole barley genome. Forty-nine barley germplasms from different geographical regions were used to detect the effectiveness of primers. And 72 co-dominant InDel markers with two alleles were selected for further genetic distance analysis in 288 barley germplasm and constructing phylogenetic tree. Thirty-two core InDel markers were screened, covering seven chromosomes of barley, with an average PIC of 0.44, and an average MAF of 0.34. The tested barley germplasms were rich in genetic diversity and could cluster most of the varieties with the same geographical origin. This study indicated that the 32 dimorphic InDel markers developed not only effectively reflect the genetic relationship between varieties, but also enrich the molecular markers used for the identification of barley varieties. The above core InDel markers have provided certain the theoretical significance and application value in on the identification of barley varieties, the relationship analysis of barley resources and group division of barley resources.

Key words: barley, re-sequencing, InDel markers, variety identification

Table 1

Information of InDel markers"

引物编号
Primer No.		 染色体
Chr.		 正向引物
Forward primer (5′-3′)		 反向引物
Reverse primer (5′-3′)		 起始位置
Start site
(bp)		 插入/缺
失碱基数
No. of
InDel base		 MAF PIC
JSB008 1H GTTCCGTCACTTCTGGCATTCG TGTCTCTCCCGCCCTCTCTTC 504080409 43 0.46 0.50
JSB011 2H CGTTGAGAAAGGATGCGGAGAC TGAAGCTGCGCGAACAAAGAC 3890795 61 0.35 0.46
JSB019 2H TGGTTGCTCTTGTTGCCGTATC GGACGCCCTAGCTGGTGATG 102398868 66 0.34 0.45
JSB021 2H ATGCTCCGTGTCCCGTATCTTG CACTGGCGTGGATCCTGACTG 672027714 40 0.37 0.46
JSB028 2H AACATGGCCTCGTGCTAACCG GCGGCGGCGTTCCGATTAG 724170740 32 0.42 0.49
JSB029 2H CTCATGGTTCGAGTTGCTTGCG GCTGCTTGTTGCTGGTGAAGG 726868326 48 0.36 0.46
JSB030 2H ACCGCTGCCAAGCCCATTC TGAAGAGCAACCGGCGATGTG 727308287 62 0.43 0.49
JSB034 2H ACCGACCGTCAGAAGCATAGC TGAGGCAAGCGAAGCGAGAG 741235783 43 0.25 0.37
JSB036 2H CAAGCGTGCGAACACTCCAAG TGCTGCCTGTGTGTGAAAAGTC 756450751 87 0.25 0.38
JSB039 3H TTAATCGATGTGACGCCTTCCG CGACAACCCTCGAAGCTTATGG 13443810 48 0.46 0.50
JSB045 3H TGCGCGAAGGTGTGGTAGTC GGCACAAAGGTGGGCTTCAAAG 653794647 34 0.20 0.31
JSB048 3H GCTCAATGGCTGATGCGTGTG GCCTCGGTGCCTCACATGG 660696828 58 0.24 0.37
JSB053 3H ATAGCCTTGCGTGCAGACAAAG TCCCAGTCCGATGACGAGAGAG 682310294 33 0.46 0.50
JSB056 3H ACCGCACAAAGTACAGTTCAGG GCGCCGGTGCTCTTCGAG 695541052 49 0.39 0.47
JSB058 4H GCTGATGGTGCTGACTTGAAGG TGGTGCTTGGCGGATACTTGG 10559789 38 0.28 0.40
引物编号
Primer No.		 染色体
Chr.		 正向引物
Forward primer (5′-3′)		 反向引物
Reverse primer (5′-3′)		 起始位置
Start site
(bp)		 插入/缺
失碱基数
No. of
InDel base		 MAF PIC
JSB060 4H GGCCTTCCGGTTCCTTGTTCC GCTCATCCGAGAAGACCTGACC 582592562 45 0.23 0.35
JSB064 5H GGCGAAGGTTCCATCTGAGCAG TGGTCGTTGTGGTTGGCGTTC 5795294 52 0.42 0.49
JSB066 5H ACAAGCAAGCGAGTTCATGTCC GCTGAGGCAAGACGGGTGTTAC 9482417 51 0.47 0.50
JSB068 5H TTTGTGCCAGCAGTTCACTGTG CCAGAGCAAAGCAACCAACATG 16013429 123 0.49 0.50
JSB070 5H TCAGTCCCACTTCCTCGTTTCC TGGAATGCCCGAACTGCTACG 187268036 83 0.24 0.36
JSB072 5H ACCCAACAGAAGGCAAGCTGAG TGGCGGGAGAGACGGGATG 454577201 44 0.34 0.45
JSB073 5H CTCGTCGCAACCGCCTCTG TCCATCCGCCTCCCTCCATTG 499579120 49 0.30 0.42
JSB075 5H ACCAGCGAGCATGAGGTCTATG AGCCACCCATGAAACCGTAGC 586304246 37 0.30 0.42
JSB077 5H TCTTCACTCTGCCTGCACTTGG AAAAGCCAGCCCATTCTTTCCC 598316392 30 0.27 0.39
JSB087 6H ATGGCAACCACAGCAACAATCC TTGCTGGTGGAGATGGTGAAAC 1170815 98 0.37 0.47
JSB090 6H AAGTCCCTGCTCGCTAGACAAG CGATGGCTCCAAGGGTTCTCC 13520409 30 0.34 0.45
JSB093 6H AGCACATCTGAGACACCGATCC TTGCCGTTTTGGTTGGTTCCTG 555335793 71 0.49 0.50
JSB105 7H CCTGGTTGGTGGTTGTCACTG TGCTGTAACGGAGAGTTTGCTC 58182221 43 0.31 0.43
JSB107 7H GCTCCAAGGTTGAATGCAAGGG GCTGGGCACAAAGCTGAAGG 600763810 63 0.29 0.42
JSB115 7H CGGTCATGGCACTTGGTTCTG GTGGGCATCCATCCAACGTAAC 647630299 67 0.32 0.44
JSB116 7H GGTTGCGAGTGAGGAACTGATG CCCGCCACCGACTTCCATG 648588179 36 0.27 0.39
JSB117 7H CATCCACGACGCCACATTGTTG TGGCTCGCTGATGGGCATTG 648931581 63 0.34 0.45

Fig. 1

Distribution of InDel primers in barley genome"

Fig. 2

Agarose gel electrophoresis of InDel markers JSB045 and JSB056 in 49 barley varieties Lanes 1 to 48 represent 48 barley materials; lane 49 represents the reference variety, Morex."

Table 2

Statistics of genetic distance"

编号
No.		 最小遗传距离
Minimum genetic distance		 最大遗传距离
Maximum genetic distance		 编号
No.		 最小遗传距离
Minimum genetic distance		 最大遗传距离
Maximum genetic distance
距离
Distance		 对应种质编号
No.		 距离
Distance		 对应种质编号
No.		 距离
Distance		 对应种质编号
No.		 距离
Distance		 对应种质编号
No.
X1 0 X2 0.70 X4 X145 0.18 X272 0.68 X15
X2 0 X1 0.70 X4 X146 0.08 X123 0.70 X107
X3 0.08 X25 0.73 X189 X147 0.13 X205 0.73 X254
X4 0.22 X29 0.70 X1 X148 0.15 X213 0.73 X262
X5 0.14 X279 0.64 X95 X149 0.08 X262 0.71 X148
X6 0.22 X31 0.73 X88 X150 0.08 X251 0.76 X189
X7 0.15 X107 0.75 X125 X151 0.10 X48 0.71 X107
X8 0.27 X9 0.74 X257 X152 0.17 X248 0.68 X114
X9 0.19 X7 0.71 X263 X153 0.13 X154 0.67 X33
X10 0.26 X205 0.71 X257 X154 0.13 X258 0.66 X107
X11 0.13 X137 0.70 X278 X155 0.15 X168 0.64 X88
X12 0.15 X68 0.70 X189 X156 0.16 X134 0.69 X95
X13 0.20 X15 0.68 X250 X157 0.14 X137 0.66 X113
X14 0.19 X53 0.73 X238 X158 0.19 X155 0.73 X88
X15 0.20 X13 0.77 X32 X159 0.13 X166 0.69 X107
X16 0.17 X162 0.68 X164 X160 0.21 X74 0.73 X139
X17 0.15 X148 0.69 X262 X161 0.16 X257 0.78 X94
X18 0.30 X242 0.68 X113 X162 0.13 X270 0.69 X189
X19 0.28 X111 0.62 X173 X163 0.21 X152 0.72 X282
X20 0.18 X226 0.68 X215 X164 0.22 X156 0.72 X176
X21 0.23 X29 0.69 X104 X165 0.19 X180 0.70 X101
X22 0.21 X171 0.70 X85 X166 0.06 X253 0.64 X4
X23 0.11 X198 0.72 X107 X167 0.06 X194 0.70 X95
X24 0.25 X20 0.68 X177 X168 0.08 X192 0.69 X15
X25 0.08 X3 0.72 X189 X169 0.05 X192 0.67 X97
X26 0.22 X13 0.67 X247 X170 0.13 X174 0.68 X107
X27 0.20 X162 0.58 X6 X171 0.21 X22 0.76 X88
X28 0.27 X114 0.67 X204 X172 0.13 X121 0.69 X88
X29 0.15 X107 0.73 X268 X173 0.32 X122 0.79 X284
X30 0.22 X80 0.66 X173 X174 0.13 X170 0.62 X106
X31 0.12 X212 0.69 X103 X175 0.13 X3 0.76 X173
X32 0.15 X44 0.77 X15 X176 0.18 X25 0.72 X107
X33 0.23 X207 0.67 X153 X177 0.30 X148 0.75 X221
X34 0.31 X28 0.68 X281 X178 0.23 X283 0.65 X221
X35 0.16 X3 0.69 X95 X179 0.21 X119 0.68 X221
X36 0.21 X82 0.68 X173 X180 0.13 X48 0.72 X107
X37 0.11 X46 0.77 X114 X181 0.18 X188 0.63 X94
X38 0.15 X279 0.68 X280 X182 0.14 X54 0.71 X106
X39 0.19 X230 0.64 X113 X183 0.25 X155 0.65 X80
X40 0.32 X66 0.69 X15 X184 0.15 X257 0.80 X94
X41 0.24 X163 0.65 X95 X185 0.21 X170 0.60 X29
X42 0.22 X57 0.71 X103 X186 0.17 X131 0.66 X107
X43 0.21 X148 0.69 X158 X187 0.18 X119 0.68 X89
X44 0.09 X67 0.70 X95 X188 0.18 X181 0.67 X65
X45 0.15 X166 0.65 X15 X189 0.24 X105 0.76 X150
X46 0.11 X37 0.76 X118 X190 0.26 X250 0.65 X9
X47 0.18 X48 0.73 X107 X191 0.08 X129 0.73 X231
X48 0.10 X151 0.72 X107 X192 0.05 X169 0.68 X97
X49 0.16 X198 0.69 X88 X193 0.16 X278 0.72 X117
X50 0.19 X123 0.72 X106 X194 0.06 X167 0.70 X95
X51 0.16 X37 0.67 X15 X195 0.13 X146 0.70 X15
X52 0.19 X84 0.75 X7 X196 0.23 X259 0.65 X106
X53 0.19 X14 0.73 X118 X197 0.14 X284 0.77 X173
X54 0 X198 0.72 X107 X198 0 X54 0.70 X107
X55 0.16 X201 0.73 X7 X199 0.22 X112 0.67 X247
X56 0.13 X235 0.69 X7 X200 0.22 X205 0.71 X247
X57 0.19 X37 0.72 X94 X201 0.06 X216 0.68 X107
X58 0.19 X54 0.66 X189 X202 0.14 X218 0.66 X88
X59 0.20 X268 0.66 X149 X203 0.09 X213 0.71 X173
X60 0.14 X223 0.68 X107 X204 0.17 X162 0.73 X276
X61 0.11 X91 0.65 X177 X205 0.13 X147 0.83 X257
X62 0.19 X234 0.75 X94 X206 0.19 X107 0.70 X125
X63 0.21 X112 0.61 X273 X207 0.06 X208 0.66 X52
X64 0.23 X5 0.66 X189 X208 0.06 X207 0.61 X173
X65 0.19 X233 0.72 X7 X209 0.21 X111 0.66 X77
X66 0.14 X46 0.71 X106 X210 0.23 X107 0.67 X125
X67 0.09 X44 0.72 X88 X211 0.21 X136 0.65 X135
X68 0.15 X72 0.65 X88 X212 0.12 X31 0.70 X95
X69 0.13 X72 0.60 X164 X213 0.09 X203 0.71 X173
X70 0.22 X69 0.64 X280 X214 0.18 X208 0.63 X204
X71 0.04 X73 0.69 X107 X215 0.27 X118 0.70 X182
X72 0.12 X198 0.63 X280 X216 0.06 X201 0.67 X107
X73 0.04 X71 0.69 X107 X217 0.29 X170 0.66 X114
X74 0.16 X198 0.71 X189 X218 0.03 X54 0.68 X107
X75 0.09 X198 0.66 X105 X219 0.19 X98 0.70 X247
X76 0.20 X87 0.74 X257 X220 0.23 X88 0.66 X263
X77 0.08 X235 0.71 X107 X221 0.18 X225 0.75 X177
X78 0.13 X225 0.69 X7 X222 0.14 X123 0.71 X15
X79 0.19 X243 0.69 X106 X223 0.13 X242 0.68 X107
X80 0.20 X38 0.69 X114 X224 0.14 X198 0.63 X107
X81 0.24 X246 0.76 X88 X225 0.11 X225 0.66 X278
X82 0.14 X84 0.70 X164 X226 0.06 X54 0.66 X107
X83 0.19 X69 0.66 X269 X227 0.25 X224 0.75 X255
X84 0.09 X73 0.71 X177 X228 0.26 X188 0.68 X177
X85 0.16 X225 0.70 X22 X229 0.17 X195 0.67 X114
X86 0.28 X154 0.63 X81 X230 0.14 X198 0.69 X189
X87 0.20 X76 0.65 X247 X231 0.16 X263 0.73 X191
X88 0.11 X89 0.76 X81 X232 0.15 X274 0.75 X114
X89 0.11 X88 0.77 X269 X233 0.13 X84 0.63 X189
X90 0.20 X94 0.71 X257 X234 0.10 X44 0.76 X94
X91 0.11 X61 0.64 X264 X235 0.08 X77 0.69 X107
X92 0.27 X82 0.69 X164 X236 0.23 X113 0.65 X52
X93 0.20 X76 0.77 X257 X237 0.23 X103 0.65 X20
X94 0.20 X90 0.80 X184 X238 0.21 X100 0.73 X14
X95 0.25 X15 0.77 X141 X239 0.15 X205 0.73 X257
X96 0.22 X93 0.72 X266 X240 0.21 X248 0.67 X13
X97 0.24 X118 0.74 X127 X241 0.16 X198 0.67 X189
X98 0.16 X99 0.69 X259 X242 0.08 X123 0.70 X114
X99 0.11 X118 0.73 X259 X243 0.18 X32 0.67 X15
X100 0.15 X103 0.77 X257 X244 0.19 X169 0.63 X20
X101 0.19 X99 0.70 X165 X245 0.22 X267 0.63 X40
X102 0.24 X250 0.70 X15 X246 0.24 X45 0.64 X88
X103 0.15 X100 0.75 X257 X247 0.23 X53 0.77 X94
X104 0.18 X103 0.74 X81 X248 0.14 X123 0.66 X280
X105 0.24 X189 0.73 X108 X249 0.13 X260 0.69 X94
X106 0.24 X116 0.74 X247 X250 0.19 X141 0.68 X13
X107 0.15 X29 0.73 X47 X251 0.08 X150 0.76 X189
X108 0.11 X285 0.73 X105 X252 0.08 X137 0.70 X97
X109 0.11 X234 0.69 X95 X253 0.06 X166 0.66 X106
X110 0.21 X56 0.63 X105 X254 0.18 X134 0.82 X205
X111 0.07 X112 0.63 X269 X255 0.27 X135 0.75 X227
X112 0.07 X111 0.66 X268 X256 0.16 X120 0.72 X114
X113 0.22 X7 0.75 X52 X257 0.15 X184 0.83 X205
X114 0.18 X118 0.77 X123 X258 0.10 X123 0.73 X107
X115 0.09 X201 0.66 X9 X259 0.20 X131 0.75 X276
X116 0.11 X118 0.70 X247 X260 0.13 X249 0.72 X94
X117 0.15 X78 0.73 X278 X261 0.17 X229 0.63 X15
X118 0.11 X99 0.76 X46 X262 0.08 X149 0.73 X148
X119 0.18 X187 0.73 X221 X263 0.16 X144 0.71 X9
X120 0.16 X256 0.66 X175 X264 0.23 X248 0.69 X106
X121 0.13 X172 0.69 X97 X265 0.13 X69 0.65 X15
X122 0.23 X143 0.66 X52 X266 0.13 X168 0.73 X15
X123 0.08 X242 0.77 X114 X267 0.20 X266 0.67 X96
X124 0.20 X157 0.66 X7 X268 0.20 X59 0.73 X106
X125 0.19 X170 0.75 X7 X269 0.19 X161 0.77 X89
X126 0.05 X143 0.66 X164 X270 0.13 X162 0.69 X189
X127 0.16 X162 0.74 X97 X271 0.17 X131 0.67 X99
X128 0.26 X9 0.66 X173 X272 0.13 X131 0.69 X114
X129 0.08 X191 0.73 X231 X273 0.29 X50 0.72 X96
X130 0.03 X133 0.73 X93 X274 0.13 X266 0.73 X114
X131 0.13 X272 0.71 X107 X275 0.25 X60 0.65 X189
X132 0.15 X222 0.66 X114 X276 0.23 X280 0.77 X161
X133 0.03 X130 0.72 X93 X277 0.17 X272 0.64 X193
X134 0.12 X212 0.69 X205 X278 0.16 X193 0.73 X117
X135 0.24 X269 0.66 X239 X279 0.14 X5 0.64 X280
X136 0.18 X168 0.68 X177 X280 0.23 X276 0.70 X150
X137 0.08 X252 0.70 X15 X281 0.21 X131 0.68 X13
X138 0.21 X250 0.70 X8 X282 0.28 X286 0.72 X163
X139 0.24 X122 0.73 X160 X283 0.21 X119 0.67 X89
X140 0.23 X46 0.70 X118 X284 0.13 X150 0.79 X173
X141 0.19 X250 0.77 X95 X285 0.11 X108 0.69 X107
X142 0.16 X260 0.72 X276 X286 0.22 X283 0.70 X88
X143 0.05 X126 0.66 X88 X287 0.30 X280 0.67 X14
X144 0.16 X263 0.72 X15 X288 0.15 X285 0.73 X107

Fig. 3

Phylogenetic tree of 288 barley germplasm based on the Neighbor-Joining (N-J) method The blue line represents the selected 49 barley germplasms."

Supplementary table 1

Information of 288 barley germplasm in present study"

编号
No.		 材料名称
Accession		 来源
Origin
X1 Morex 美国 USA
X2 鄂941049 E 941049 中国湖北 Hubei, China
X3 AC Bacon 加拿大 Canada
X4 四国裸89号 Siguoluo 89 日本 Japan
X5 AC Burman 加拿大 Canada
X6 行幸大麦 Xingxingdamai 日本 Japan
X7 旱地兰 Handilan 中国 China
X8 宜平四棱大麦 Yipingsilengdamai 中国云南 Yunnan, China
X9 柱大麦 Zhudamai 中国河南 Henan, China
X10 C2118 加拿大 Canada
X11 品H0909 Pin H0909 中国北京 Beijing, China
X12 济皮02 Jipi 02 中国山东 Shandong, China
X13 孔措蓝 Kongcuolan 中国西藏 Tibet, China
X14 GAIRDNER 澳大利亚 Australia
X15 龙中蓝 Longzhonglan 中国西藏 Tibet, China
X16 鬼怒3号 Guinu 3 日本 Japan
X17 克山城区大麦 Keshanchengqudamai 中国黑龙江 Heilongjiang, China
X18 武威黑大麦 Wuweiheidamai 中国甘肃 Gansu, China
X19 倍取10号 Beiqu 10 日本 Japan
X20 驻87-3-2 ZHU 87-3-2 中国河南 Henan, China
X21 安吉落芒毛大麦 Anjiluomangdamai 中国浙江 Zhejiang, China
X22 八楞子大麦 Balengzidamai 中国 China
X23 盐91128 Yan 91128 中国江苏 Jiangsu, China
X24 长芒六棱露仁 Changmangliulengluren 中国 China
X25 西海皮44号 Xihaipi 44 日本 Japan
X26 沪麦4号 Humai 4 中国上海 Shanghai, China
X27 洛松 Luosong 中国 China
X28 耐盐C2118 Naiyan C2118 中国北京 Beijing, China
X29 嵊县无芒六棱 Shengxianwumangliuleng 中国浙江 Zhejiang, China
X30 天台白四棱 Tiantaibaisileng 中国浙江 Zhejiang, China
X31 新登蒙古麦 Xindengmenggumai 中国内蒙古 Inner Mongolia, China
X32 秀9560 Xiu 9560 中国浙江 Zhejiang, China
X33 永嘉元麦 Yongjiayuanmai 中国浙江 Zhejiang, China
X34 崇信春大麦 Chongxinchundamai 中国甘肃 Gansu, China
X35 鉴35 (元麦) Jian 35 (Yuanmai) 中国江苏 Jiangsu, China
X36 北青三号 Beiqing 3 中国青海 Qinghai, China
X37 美丽黄金 Meilihuangjin 日本 Japan
X38 MORRISON 美国 USA
X39 宽颖裸麦 Kuanyinluomai 中国 China
X40 懒黄种 Lanhuangzhong 中国西藏 Tibet, China
X41 沪01-2946 Hu 01-2946 中国上海 Shanghai, China
X42 哈铁系1号 Hatiexi 1 中国黑龙江 Heilongjiang, China
X43 短钩裸大麦 Duangouluodamai 中国 China
X44 秀麦2号 Xiumai 2 中国浙江 Zhejiang, China
X45 新啤一号 Xinpi 1 中国新疆 Xinjiang, China
X46 关东二条19号 Gongdong’ertiao 19 日本 Japan
X47 TALLON 澳大利亚 Australia
X48 KAPUTOR 澳大利亚 Australia
X49 星胜NO.1 Xingsheng NO.1 中国山东 Shandong, China
X50 草麦 Caomai 中国 China
X51 港啤1号 Gangpi 1 中国江苏 Jiangsu, China
X52 缟检-4 Gaojian 4 日本 Japan
X53 SCHOONER 澳大利亚 Australia
X54 Mikanwgold 日本 Japan
X55 莆895067 Pu 895067 中国福建 Fujian, China
X56 浙皮1号 Zhepi 1 中国浙江 Zhejiang, China
X57 NAMOI 美国 USA
X58 通麦10号 Tongmai 10 中国江苏 Jiangsu, China
X59 Gobernadora 英国 Britain
X60 吉8701 Ji 8701 中国吉林 Jilin, China
X61 乐清洋大麦 Yueqingyangdamai 中国浙江 Zhejiang, China
X62 米麦114 Mimai 114 中国浙江 Zhejiang, China
X63 矮脚二棱 Aijiao’erleng 中国 China
X64 扬饲麦1号 Yangsimai 1 中国江苏 Jiangsu, China
X65 粑地虎 Padihu 中国 China
X66 单二 Sahner 中国江苏 Jiangsu, China
X67 花11 Hua 11 中国上海 Shanghai, China
X68 蒙啤一号 Mengpi 1 中国内蒙古 Inner Mongolia, China
X69 BARI287 —
X70 TRADITION 美国 USA
X71 LEGACY 加拿大 Canada
X72 COLLINS —
X73 盐05023 Yan 05023 中国江苏 Jiangsu, China
X74 扬辐7110 Yangfu 7110 中国江苏 Jiangsu, China
X75 如东203-1 Rudong 203-1 中国江苏 Jiangsu, China
X76 莆848104 Pu 848104 中国福建 Fujian, China
X77 驻06095-2-1 Zhu 06095-2-1 中国河南 Henan, China
X78 鄂大麦32122 Edamai 32122 中国湖北 Hubei, China
X79 黑09-26 Hei 09-26 中国黑龙江 Heilongjiang
X80 驻大麦5号 Zhudamai 5 中国河南 Henan, China
X81 印度矮生 Yindu’aisheng 印度 India
X82 通0306 Tong 0306 中国江苏 Jiangsu, China
X83 保大麦13号 Baodamai 13 中国云南 Yunnan, China
X84 盐2013品15 Yan 2013 Pin 15 中国江苏 Jiangsu, China
X85 盐黑1号 Yanhei 1 中国江苏 Jiangsu, China
X86 萨加青稞 Sajiaqingke 中国青海 Qinghai, China
X87 湟源蓝青稞 Huangyuanlanqingke 中国青海 Qinghai, China
X88 西宁红服泥 Xininghongfuni 中国青海 Qinghai, China
X89 无皮青稞 (甘南) Wupiqingke (Gannan) 中国甘肃 Gansu, China
X90 甘孜白六棱Ganzibailiuleng 中国四川 Sichuan, China
X91 苏裸麦1号 Suluomai 1 中国青海 Qinghai, China
X92 勾芒白青稞 Goumangbaiqingke 中国青海 Qinghai, China
X93 长身子青稞 (甘南) Changshenziqingke (Gannan ) 中国青海 Qinghai, China
X94 藏830563 Zang 830563 中国西藏 Tibet, China
X95 足捉春青稞 (甘孜) Zuzhuochunqingke (Ganzi) 中国四川 Sichuan, China
X96 甘青1号 Ganqing 1 中国甘肃 Gansu, China
X97 珉县青稞 Minxianqingke 中国青海 Qinghai, China
X98 循化黑青稞 Xunhuaheiqingke 中国青海 Qinghai, China
X99 矮杆齐 Aiganqi 中国青海 Qinghai, China
X100 民和四棱蓝青稞 Minhesilenglanqingke 中国青海 Qinghai, China
X101 小金沙龙狗尾 (甘孜) Xiaojinshalonggouwei (Ganzi) 中国四川 Sichuan, China
X102 涅如姆扎ZDM7701 Nierumuza ZDM7701 中国西藏 Tibet, China
X103 大粒98-714 Dali 98-714 墨西哥 Mexico
X104 权青稞 Quanqingke 中国西藏 Tibet, China
X105 大通白六棱 Datongbailiuleng 中国青海 Qinghai, China
X106 小青稞 (金塔) Xiaoqingke (Jinta) 中国甘肃 Gansu, China
X107 嵊县无芒大麦 Shengxianwumangdamai 中国浙江 Zhejiang, China
X108 浙原18 Zheyuan 18 中国浙江 Zhejiang, China
X109 秀麦3号 Xiumai 3 中国浙江 Zhejiang, China
X110 嵊县209 Shengxian 209 中国浙江 Zhejiang, China
X111 临海光头大麦 Linhaiguangtoudamai 中国浙江 Zhejiang, China
X112 玉环洋大麦 Yuhuanyangdamai 中国浙江 Zhejiang, China
X113 紫皮大麦 Zipidamai 中国 China
X114 新民大麦 Xinmindamai 中国 China
X115 甘木二条 Amagi Nijo 日本 Japan
X116 冬青15号Dongqing 15 中国西藏 Tibet, China
X117 浙农白壳 Zhenongbaike 中国浙江 Zhejiang, China
X118 藏83062 Zang 83062 中国西藏 Tibet, China
X119 Mirra 德国 Germany
X120 MARION 德国 Germany
X121 KASKADE 德国 Germany
X122 HALCYON 英国 Britain
X123 Micmac 加拿大 Canada
X124 TOKAK 土耳其 Turkey
X125 FLAMENCO 法国 France
X126 PANDA 法国 France
X127 KARAT 捷克 Czekh
X128 ZAVET-3 苏联 the Soviet Union
X129 HORDEUM SPONTANEUM VAR SPONTANEUM 德国 Germany
X130 ATHS 埃及 Egypt
X131 SHANNON 澳大利亚 Australia
X132 ALFA 丹麦 Denmark
X133 ALKAR 克罗地亚 Croatia
X134 ARIANA 突尼斯 Tunis
X135 ATHENAIS 塞浦路斯 Cyprus
X136 BANNACKY II 捷克 Czekh
X137 BOHEMIAN 奥地利 Austria
X138 Boyer 美国 USA
X139 BUCHER 伊拉克 Iraq
X140 CHEVRON 瑞士 Japan
X141 FREJA 瑞典 Japan
X142 Glacier 美国 USA
X143 ISABELLA 德国 Germany
X144 DIAMOND 加拿大 Canada
X145 CAMEO 英国 Britain
X146 BONITA 乌拉圭 Ukraine
X147 ARABIAN BLUE 澳大利亚 Australia
X148 BARBLESS 美国 USA
X149 BLACK 2 ROW-HULLED —
X150 CLAUDE 加拿大 Canada
X151 FAVORIT 捷克斯洛伐克 Czechoslovakia
X152 GOLDTHORPE SPRATT 英国 Britain
X153 HATVANI 匈牙利 Hungary
X154 HEINES HANNA 德国 Germany
X155 HILAND 美国 USA
X156 ORGE (ESPERANCE) 摩洛哥 Morocco
X157 PUSA NO.1 印度 India
X158 RAGUSA B 前南斯拉夫Yugoslavia
X159 SLOVAK 加拿大 Canada
X160 SPARTAN (DEHISCENT AWN) 美国 USA
X161 STEVELAND 美国 USA
X162 FORREST (USA) 美国 USA
X163 ULANDRA 澳大利亚 Australia
X164 SHERPA 英国 Britain
X165 H. DISTICHUM NUTANS 葡萄牙 Portuguesa
X166 CANADA 574234 加拿大 Canada
X167 JAPAN 47C11-24 日本 Japan
X168 H.SPONT. X-15 以色列 Israel
X169 HERMON 以色列 Israel
X170 WINCHESTER 美国 USA
X171 TAPGOLBORI 韩国 Korea
X172 HOHENFINOWER 德国 Germany
X173 TURKEY 568 土耳其 Turkey
X174 GOLDIE 瑞典 Japan
X175 HEITPAS-5 美国 USA
X176 MAMLUK 苏联 the Soviet Union
X177 DAEJINBORI 韩国 Korea
X178 CHOCHALUNG 尼泊尔 Nepal
X179 VIOLA 德国 Germany
X180 ARUPO S 墨西哥 Mexico
X181 PALLIDUM 043 乌克兰 Ukraine
X182 RUSSIA 美国 USA
X183 KHEMUS 保加利亚 Bulgaria
X184 WHITE NAKED ATLAS - SUSPECT 埃塞俄比亚 Ethiopia
X185 LUKJANOVAE 苏联 the Soviet Union
X186 CHEPUA 尼泊尔 Nepal
X187 AKHELOL 1 保加利亚 Bulgaria
X188 H.VULG. VAR PALLIDUM 阿尔及利亚 Algeria
X189 藏青85 Zangqing 85 中国西藏 Tibet, China
X190 RIHANE 'S' 叙利亚 Syria
X191 SPONTANEUM 2 伊朗 Iran
X192 KELT 以色列 Israel
X193 九州二条 Jiuzhou’ertiao 日本 Japan
X194 三月黄 Sanyuehuang 中国浙江 Zhejiang, China
X195 Araplies 加拿大 Canada
X196 京裸11 Jingluo 11 中国北京 Beijing, China
X197 宾县皮4号 Binxianpi 4 中国四川 Sichuan, China
X198 盐选1号 Yanxuan 1 中国江苏 Jiangsu, China
X199 六棱黑青稞 Liulengheiqingke 中国西藏 Tibet, China
X200 龙中黄 Longzhonghuang 中国西藏 Tibet, China
X201 关东二条22号 Gongdongertiao 22 日本 Japan
X202 Kinuya tata 日本 Japan
X203 拜泉皮1号 Baiquanpi 1 中国黑龙江 Heilongjiang, China
X204 美97-1338 Mei 97-1338 美国 USA
X205 麻青稞 Maqingke 中国甘肃 Gansu, China
X206 济南火烧头露仁 Jinanhuoshaotouluren 中国山东 Shandong, China
X207 绥定青稞 Suidingqingke 中国四川 Sichuan, China
X208 泰顺四棱米麦 Taishunsilengmimai 中国浙江 Zhejiang, China
X209 义乌早大麦 Yiwuzaodamai 中国浙江 Zhejiang, China
X210 御岛裸 Yudaoluo 日本 Japan
X211 红露仁青稞 Honglurenqingke 中国陕西 Shaanxi, China
X212 黄青稞 Huangqingke 中国甘肃 Gansu, China
X213 U.S.D.A.2474 美国 USA
X214 临海老来红 Linhailaolaihong 中国浙江 Zhejiang, China
X215 绵阳87-10 Mianyang 87-10 中国四川 Sichuan, China
X216 不知梅雨 Buzhimeiyu 日本 Japan
X217 野生皮大麦-1 Yeshengpidamai -1 中国西藏 Tibet, China
X218 乐啤4号 Lepi 4 中国四川 Sichuan, China
X219 紫青稞 Ziqingke 中国青海 Qinghai, China
X220 POCO 澳大利亚 Australia
X221 凉城大粒洋大麦 Liangchengdaliyangdamai 中国内蒙古 Inner Mongolia, China
X222 敖德萨 Aodesa 苏联 the Soviet Union
X223 KLAGES 美国 USA
X224 浙农大3号 Zhenongda 3 中国浙江 Zhejiang, China
X225 矮秆-4 Aigan-4 中国 China
X226 早熟3号 Zaoshu 3 日本 Japan
X227 单3 Shan 3 中国江苏 Jiangsu, China
X228 驻112 Zhu 112 中国河南 Henan, China
X229 甘啤2号 Ganpi 2 中国甘肃 Gansu, China
X230 沪麦12号 Humai 12 中国上海 Shanghai, China
X231 通鉴43 Tongjian 43 中国江苏 Jiangsu, China
X232 LARA 澳大利亚 Australia
X233 矮壮21 Aizhuang 21 中国江苏 Jiangsu, China
X234 丰农啤1号 Fengnongpi 1 中国江苏 Jiangsu, China
X235 扬农啤2号 Yangnongpi 2 中国江苏 Jiangsu, China
X236 盐丰1号 Yanfeng 1 中国江苏 Jiangsu, China
X237 康青9号 Kangqing 9 中国四川 Sichuan, China
X238 昆仑14 Kunlun 14 中国青海 Qinghai, China
X239 民和长芒青稞 Minhechangmangqingke 中国青海 Qinghai, China
X240 Rainbow 英国 Britain
X241 AKKA 瑞典 Sweden
X242 Birka 加拿大 Canada
X243 WINTERMALT 美国 USA
X244 GALLEON 澳大利亚 Australia
X245 MELUSINE 法国 France
X246 ABLYN 澳大利亚 Australia
X247 ABUSIR 埃及 Egypt
X248 ADORRA 奥地利 Austria
X249 AIM 埃及 Egypt
X250 AKELA 德国 Germany
X251 AKER 美国 USA
X252 ALBERT 法国 France
X253 ALBERTA 1 加拿大 Canada
X254 ALGERIA 6 阿尔及利亚 Algeria
X255 ANOIDIUM 阿根廷 Argentina
X256 ARAMIR 荷兰 Holland
X257 ARGENTINE 阿根廷 Argentina
X258 ASPA 德国 Germany
X259 BADGER 墨西哥 Mexico
X260 BALODI 英国 Britain
X261 BERAC 荷兰 Holland
X262 BLACK EGYPTIAN 美国 USA
X263 Gateway 加拿大 Canada
X264 GOLDMARKER 英国 Britain
X265 VISTA 英国 Britain
X266 PARWAN 澳大利亚 Australia
X267 CHALET 瑞士 Japan
X268 EMMA 荷兰 Holland
X269 FAMESH 美国 USA
X270 GAZELLE 加拿大 Canada
X271 GOLDEN MELON 日本 Japan
X272 GUYMALAYE 丹麦 Denmark
X273 HEY SPECIAL 葡萄牙 Portuguesa
X274 HISPONT 德国 Germany
X275 LUBAS 伊拉克 Iraq
X276 RASPUT 俄罗斯 Russia
X277 GOLD MEDAL 德国 Germany
X278 ACUMAI-89 巴西 Brazil
X279 AGNETA 瑞典 Japan
X280 GURZAN 阿富汗 Afghanistan
X281 JASPIS (SPRING) 捷克 Czekh
X282 LAND RACE 3832/29 澳大利亚 Australia
X283 KIMALUNG 尼泊尔 Nepal
X284 海盐大麦 Haiyandamai 中国浙江 Zhejiang, China
X285 泰兴8425 Taixing 8425 中国江苏 Jiangsu, China
X286 PUSA R1 印度 India
X287 CHALUS 伊朗 Iran
X288 Franklin 澳大利亚 Australia
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