叉柱棉sHSP基因家族的鉴定与特征分析

doi:10.3724/SP.J.1006.2021.04235

Abstract

Abstract:

Small heat shock protein (sHSP) has the lowest molecular weight in heat shock protein and sHSP has the highly conserved α-crystallin domain. The sHSP family is very important in response to various stresses, but the identification and analysis of sHSP gene family in Gossypioides kirkii have not been reported. In this study, we identified 39 GksHSPs with 10 subfamilies in Gossypioides kirkii. There were seven gene duplication events in GksHSP family and all of duplicated gene pairs were involved in segmental duplication events. The cotton-specific whole genome duplication event primarily resulted in the GksHSP expansion. The GksHSP expansion was also related to the protein kinase, mitochondrial carrier protein, and auxin responsive protein. Besides, GksHSP members might respond to various stresses by ABA/MeJA-mediated pathways, and GksHSP26 and its corresponding orthologous genes in Gossypium hirsutum were very important in stress responses. These results could provide the theoretical basis of the breeding of stress tolerance in Gossypioides kirkii and cotton.

Key words: Gossypioides kirkii, sHSP, identification, expansion, function

FAN Kai, PAN Xin-Feng, MAO Zhi-Jun, YE Fang-Ting, LI Zhao-Wei, LIN Wei-Wei, LIN Wen-Xiong. Identification and analysis of sHSP gene family in Gossypioides kirkii[J].Acta Agronomica Sinica, 2021, 47(10): 1913-1926.

Figures/Tables 10

Fig. 1

Table 1

Identification and structural analysis of GksHSP in this study"

名称 Name	位点名称 Locus name	亚家族 Subfamily	蛋白质长度 Protein length (aa)	分子量 Molecular weight (kD)	理论pI Theoretical pI	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-turn	无规则卷曲 Random coil
GksHSP01	Kirkii_Version3_Juiced.00g028900	CP	144	16.32340	4.95	23	30	6	85
GksHSP02	Kirkii_Version3_Juiced.00g047410	CI	159	18.32872	5.98	28	29	11	91
GksHSP03	Kirkii_Version3_Juiced.00g056780	ER	187	21.44579	6.77	64	31	8	84
GksHSP04	Kirkii_Version3_Juiced.00g059950	CI	153	17.69095	5.35	34	28	7	84
GksHSP05	Kirkii_Version3_Juiced.00g085690	CII	159	18.01872	6.64	52	34	8	65
GksHSP06	Kirkii_Version3_Juiced.00g085700	CII	156	17.66154	6.62	53	38	11	54
GksHSP07	Kirkii_Version3_Juiced.00g091700	CI	156	17.91837	5.79	38	30	10	78
GksHSP08	Kirkii_Version3_Juiced.00g091710	CI	156	17.80026	6.18	36	29	9	82
GksHSP09	Kirkii_Version3_Juiced.00g092160	CI	133	14.79902	9.00	40	22	5	66
GksHSP10	Kirkii_Version3_Juiced.00g096000	MTII	246	27.95450	7.80	82	46	13	105
GksHSP11	Kirkii_Version3_Juiced.00g097580	CIII	102	11.37751	4.37	35	16	5	46
GksHSP12	Kirkii_Version3_Juiced.00g101120	CIV	139	15.76492	5.34	28	29	10	72
GksHSP13	Kirkii_Version3_Juiced.00g120690	CI	159	18.25460	6.18	20	34	10	95
GksHSP14	Kirkii_Version3_Juiced.00g120700	CI	159	18.19460	6.18	28	31	10	90
GksHSP15	Kirkii_Version3_Juiced.00g120710	CI	159	18.27965	6.64	29	32	8	90
GksHSP16	Kirkii_Version3_Juiced.00g134580	CIV	139	15.85984	4.61	30	34	6	69
GksHSP17	Kirkii_Version3_Juiced.00g136170	CII	159	18.18400	6.19	49	31	7	72
GksHSP18	Kirkii_Version3_Juiced.00g136180	CII	153	17.05883	8.59	53	40	9	51
GksHSP19	Kirkii_Version3_Juiced.00g184450	CI	156	17.82725	5.77	32	31	10	83
GksHSP20	Kirkii_Version3_Juiced.00g184460	CI	160	18.36475	6.18	39	33	8	80
GksHSP21	Kirkii_Version3_Juiced.00g184750	CI	135	14.96507	5.91	38	33	4	60
GksHSP22	Kirkii_Version3_Juiced.00g188220	CI	143	16.28479	9.22	25	28	7	83
GksHSP23	Kirkii_Version3_Juiced.00g193540	PX	165	18.81639	5.98	18	40	8	99
GksHSP24	Kirkii_Version3_Juiced.00g194110	CII	158	17.75257	5.97	51	34	11	62
GksHSP25	Kirkii_Version3_Juiced.00g209640	CP	232	25.77139	6.84	39	43	8	142
GksHSP26	Kirkii_Version3_Juiced.00g215570	CI	159	18.38593	5.83	30	30	10	89
GksHSP27	Kirkii_Version3_Juiced.00g230720	SINGLE	140	16.28668	5.39	28	26	10	76
GksHSP28	Kirkii_Version3_Juiced.00g244800	CI	163	18.52218	8.83	33	36	10	84
GksHSP29	Kirkii_Version3_Juiced.00g246020	CI	156	17.87539	6.76	27	39	10	80
GksHSP30	Kirkii_Version3_Juiced.00g256930	CI	144	16.53776	5.58	26	34	8	76
GksHSP31	Kirkii_Version3_Juiced.00g267060	CV	166	19.59569	6.33	57	39	17	53
GksHSP32	Kirkii_Version3_Juiced.00g273810	CI	93	10.50390	4.61	11	32	9	41
GksHSP33	Kirkii_Version3_Juiced.00g301400	PX	141	15.98226	6.92	24	36	10	71
GksHSP34	Kirkii_Version3_Juiced.00g331020	CP	230	25.96835	5.86	40	38	9	143
GksHSP35	Kirkii_Version3_Juiced.00g336540	MTI/CP	181	20.93121	6.23	61	35	10	75
GksHSP36	Kirkii_Version3_Juiced.00g336590	MTI/CP	327	36.83181	5.49	103	48	22	154
GksHSP37	Kirkii_Version3_Juiced.00g343620	CI	149	17.03446	7.83	30	35	9	75
GksHSP38	Kirkii_Version3_Juiced.00g375140	ER	184	20.66178	5.76	57	33	8	86
GksHSP39	Kirkii_Version3_Juiced.00g377690	CV	196	22.48230	5.45	44	40	17	95

Table 1

Fig. 2

Table 2

Fig. 3

Table 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

References 42

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基序ID Motif ID	基序序列 Motif sequence	基序长度 Motif length (aa)
MEME-1	RIDWKETPEAHVFKADVPGLKKEEVKVEVEDDRVLQISGERNVEKEDKNDT	51
MEME-2	NAKMDQIKASMEBGVLTVTVP	21
MEME-3	ERSSGKFMRRFRLPE	15
MEME-4	MAMIPSFFGNRRSSI	15
MEME-5	FDPFSLDVWDPFKDF	15
MEME-6	HVFKADLPGLKKEEVKVEVED	21
MEME-7	KLEVKKPDVKAIEIS	15
MEME-8	SLSTRSPETSAFVNA	15
MEME-9	MLDIPDETEKSPNAPSRAYVRDAKAMAAT	29
MEME-10	MEFPFPSDQHSPLYHYLLPSPPLFSNQLLPENHLNWTQTP	40
MEME-11	QQREGKKKDWRSCNWWEYGYVRRLELPZDADWRKIEAFLSNDVVLEIRIPRN	52
MEME-12	MDFRIMGFDSPLLHT	15
MEME-13	WTNRSYSSYBTSLQLPD	17
MEME-14	VLVIKGERKEE	11
MEME-15	QERAVEKRPKRLAMDVSPFGLLDPLSPMRSMRQMLDTMDRIFEDAMIFPGSNRRQGG	57
MEME-16	DWKETPEA	8
MEME-17	PFSVSFPSKNPCNSRLSVVRAZAAGDNNKDTSVDVHVNKDN	41
MEME-18	GSFYIDPADVPDRVEVLARAA	21
MEME-19	NIQIHVEKGKIMEIFGQ	17
MEME-20	RAPWDIKDGEH	11

复制基因1 Duplicated gene 1	复制基因2 Duplicated gene 2	亚家族 Subfamily	非同义替换率 Ka	同义替换率 Ks	非同义替换率/同义替换率 Ka/Ks	纯化选择 Purifying selection	复制类型 Duplicated type
GksHSP05	GksHSP17	CII	0.063959	0.809673	0.078994	是Yes	片段复制事件 Segmental
GksHSP06	GksHSP18	CII	0.090699	0.742610	0.122136	是Yes	片段复制事件Segmental
GksHSP07	GksHSP20	CI	0.045458	0.629437	0.072220	是Yes	片段复制事件Segmental
GksHSP09	GksHSP21	CI	0.159061	0.715073	0.222441	是Yes	片段复制事件Segmental
GksHSP06	GksHSP24	CII	0.113536	1.039721	0.109199	是Yes	片段复制事件Segmental
GksHSP18	GksHSP24	CII	0.103983	0.730373	0.142370	是Yes	片段复制事件Segmental
GksHSP25	GksHSP34	CP	0.125830	0.606857	0.207347	是Yes	片段复制事件Segmental

Identification and analysis of sHSP gene family in Gossypioides kirkii

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