Fig. 1

Association analysis of seed oil content and fatty acid components based on BnSnRK in rapeseed The figure shows the summary of the association analysis results under the looser screening conditions. There were eight layers of dotted labeling, and the phenotypes from inner to outer circles in order were oil content, palmitic acid, stearic acid, oleic acid, linoleic acid, linoleic acid, linolenic acid, erucic acid, and arachidonic acid. The square label shows the composition of 18 carbon fatty acids."

Fig. s2

Comparison of physical and chemical properties of SnRK subfamilies"

Table s1

Secondary structure analysis of BnSnRK protein (%)"

Fig. s3

Enrichment analysis of BnSnRK gene family members"

Fig. s1

Prediction of subcellular localization of SnRK gene family"

Fig. s4

Copert-acting element analysis of BnSnRK family promoter MYB: MYB tran-scription factor response element; MYC: MYC transcription factor response element; MBS: MYB binding site."

Fig. 2

Phylogenetic tree, CDD conservative structures, and gene structures of representative members of BnSnRK gene family The BnSnRK gene family are divided into three subfamilies: the red region represents SnRK1 subfamily, the blue region represents SnRK2 subfamily, and the yellow region represents SnRK3 subfamily."

Fig. s5

Conservative motif analysis of some members of BnSnRK"

Fig. 10

Fig. S6 Manhattan diagram of association analysis of seed oil content in E4 environment"

Table 1

Repeatedly detected sites associated with seed oil content of rapeseed"

Fig. s7

Haplotype analysis boxplot Haplotype ACT and haplotype CTC were the haplotypes with the lowest and highest oil content of gene BnaA10g28850D, respectively, and were represented by green boxes in E2, E4, and E5 environments. Haplotype GGGT and TAAC were the haplotypes with the lowest and highest oil content of gene BnaA07g12290D, respectively. The borders of the boxes in each environment are colored differently."

Table s2

Multiple comparisons of haplotype analysis"

Fig. 3

Expression levels of oil-related candidate genes in the seed of Brassica napus A: expression of oil-related candidate genes of BnSnRK gene family in young seeds of rapeseed detected by qRT-PCR; B: relative expression of all candidate genes."

Fig. s8

Chromosome location distribution of SnRK gene family In the figure, the member names of BnSnRK1, BnSnRK2, and BnSnRK3 subgroup are represented by green, red, and black respectively. Random chromosome without member distribution does not appear in the figure."

Fig. 4

Homologous comparison of SnRK gene family in Brassica napus (A) and collinear association of cabbage, Brassica napus, and Chinese cabbage (B) The light blue background line in figure A is a collinearity gene pair between SnRK gene family members of Brassica napus. The color line will highlight gene member that may be related to the seed oil content of Brassica napus. In figure B, the gray background line represents the collinear block in the whole genome, and the highlighted line represents the collinear relationship between the BnSnRK gene family members in three species."