Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (3): 580-589.doi: 10.3724/SP.J.1006.2022.11015

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene

FU Mei-Yu1,2(), XIONG Hong-Chun2, ZHOU Chun-Yun2, GUO Hui-Jun2, XIE Yong-Dun2, ZHAO Lin-Shu2, GU Jia-Yu2, ZHAO Shi-Rong2, DING Yu-Ping2, XU Yan-Hao1,*(), LIU Lu-Xiang2,*()   

  1. 1Hubei Collaborative Innovation Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
  • Received:2021-02-03 Accepted:2021-06-16 Online:2022-03-12 Published:2021-06-28
  • Contact: XU Yan-Hao,LIU Lu-Xiang E-mail:17862825179@163.com;xyh09@yangtzeu.edu.cn;liuluxiang@caas.cn
  • Supported by:
    National Natural Science Foundation of China(31801346);Chinese Academy of Agricultural Sciences Basal Research Fund(Y2020YJ09);National Key Research and Development Program of China(2016YFD0102100)

Abstract:

Lodging easily causes severe decrease in wheat yields. Identification and utilization of favorable dwarfing genes is the key to develop new varieties with high yield and lodging resistance. In this study, a dwarf mutant je0098 as material was induced by EMS mutagenesis from Jing 411 (WT) and had fine characteristics in yield components. We mapped the dwarfing gene through genetic analysis of plant height, and combining with exon capture sequencing and genetic linkage analysis. Statistical analyses of plant height in three-year field experiment suggested that plant height of je0098 was 15 cm lower than that of WT. Histocytological analysis of je0098 and WT indicated that the internode cell length of je0098 was about 18% shorter than that of WT, suggesting that the shorter internode cell length caused the dwarfism of je0098. Gibberellic acid treatment showed that je0098 was a gibberellic acid-sensitive dwarf mutant. An F2 segregation population consisting of 344 individuals was constructed by crossing WT and je0098. Combining with the phenotypic data of F2:3 families, dwarf homozygous and tall individuals were selected to construct progeny pools. Exon capture sequencing was performed on the two parents and progeny pools, respectively. A quantitative trait locus (QTL) with effects on reduced height was identified on chromosome 2D. Based on SNPs detected by genome-wide sequencing, six KASP markers were developed on chromosome 2D to genotype F2 individuals. Genetic linkage map was constructed using QTL IciMapping. Combining with phenotype data of three-year field experiment, the dwarfing gene was mapped in the range of 20.77-28.84 Mb with genetic distance of 11.48 cM. These results will lay the foundation for further functional research of je0098 and its application in wheat breeding.

Key words: wheat, plant height, dwarf gene, BSA, molecular marker

Fig. 1

Phenotype comparison of wild type (WT) and mutant je0098 * indicates significant difference between wild type and the mutant at the 0.05 probability level; ** indicates significant difference between wild type and the mutant at the 0.01 probability level."

Fig. 2

Comparison of internode cell length and gibberellin sensitivity between wild type (WT) and mutant je0098 * indicates significant difference at the 0.05 probability level; ** indicates significant difference at the 0.01 probability level."

Table 1

Plant height of F2:3 family for two years"

年份
Year
表型
Phenotype
观察值
Observed count (O)
期望值
Expected count (E)
(O-E)2/E χ2 P (df=1)
2019 矮秆表型 Dwarf phenotype 68 83.5 2.877 3.836 0.050
非矮秆表型 Non-dwarf phenotype 266 250.5 0.959
合计 Total 334 334.0 3.836
2020 矮秆表型 Dwarf phenotype 69 82.5 2.209 2.945 0.086
非矮秆表型 Non-dwarf phenotype 261 247.5 0.736
合计 Total 330 330.0 2.945

Fig. 3

Fitting chart of Euclidean Distance correlation analysis The red dotted horizontal line indicates the 99th percentile of the fitness value."

Fig. 4

Linkage map of QTL and its corresponding physical map on 2D chromosome Red line indicates the LOD curve identified by phenotype data from plant height of J411/je0098 F2 population in 2018, green line and blue line indicate LOD curves identified by phenotype data from average plant height of J411/je0098 F2:3 families in 2019 and 2020, respectively."

Fig. 5

Fig. S1 Comparison of agronomic traits between WT and je0098 in 2015 Thousand-kernel weight and spike length of WT and je0098 in Zhongpuchang and Changing experimental sites: (A) thousand-kernel weight; (B) spike length. ** indicates significant difference at the 0.01 probability level."

Table 2

Functional prediction of SNP loci in candidate interval"

基因
Gene
突变位点
Position (bp)
野生型
Wild type
突变型
Mutated type
突变类型
Mutation type
基因功能
Gene function
TraesCS2D01G059800 24,925,674 C T 错义突变
Missense mutant
RNA结合家族蛋白
RNA-binding family protein
TraesCS2D01G062900 26,616,391 C T 错义突变
Missense mutant
PHD锌指蛋白
PHD finger protein
TraesCS2D01G052400LC 22,812,351 C T UTR突变
UTR mutant
MBOAT家族蛋白
MBOAT family protein
TraesCS2D01G053600LC 23,479,083 C T 内含子突变
Intron mutant
类烯醇化酶
Enolase-like
[1] Fan M, Shen J, Yuan L, Jiang R, Chen X, Davies W J, Zhang F. Improving crop productivity and resource use efficiency to ensure food security and environmental quality in China. J Exp Bot, 2012, 63:13-24.
doi: 10.1093/jxb/err248
[2] Wan J. Genetic Crop Improvement: a guarantee for sustainable agricultural production. Engineering, 2018, 4:431-432.
doi: 10.1016/j.eng.2018.07.019
[3] Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 1999, 400:256-261.
doi: 10.1038/22307
[4] 蒋梦婷, 渠慎春. DELLA蛋白在植物生长发育中的作用. 西北植物学报, 2018, 38:1952-1960.
Jiang M T, Qu S C. DELLA and its functions in plant growth and development. Acta Bot Boreal-occident Sin, 2018, 38:1952-1960 (in Chinese with English abstract).
[5] Hauvermale A L, Ariizumi T, Steber C M. Gibberellin signaling: a theme and variations on DELLA repression. Plant Physiol, 2012, 160:83-92.
doi: 10.1104/pp.112.200956 pmid: 22843665
[6] Daviere J M, Achard P. A pivotal role of DELLAs in regulating multiple hormone signals. Mol Plant, 2016, 9:10-20.
doi: 10.1016/j.molp.2015.09.011
[7] Hedden P. The genes of the green revolution. Trends Genet, 2003, 19:5-9.
pmid: 12493241
[8] Würschum T, Langer S M, Longin C F. Genetic control of plant height in European winter wheat cultivars. Theor Appl Genet, 2015, 128:865-874.
doi: 10.1007/s00122-015-2476-2 pmid: 25687129
[9] Chen G, Zheng Q, Bao Y, Liu S, Wang H, Li X. Thinopyrum ponticum chromatin Thinopyrum ponticum chromatin. J Biosci, 2012, 37:149-155.
doi: 10.1007/s12038-011-9175-1
[10] Zhao K, Xiao J, Liu Y, Chen S, Yuan C, Cao A, You F M, Yang D, An S, Wang H, Wang X. Rht23 (5Dq') likely encodes a Q homeologue with pleiotropic effects on plant height and spike compactness. Theor Appl Genet, 2018, 131:1825-1834.
doi: 10.1007/s00122-018-3115-5
[11] Peng Z S, Li X, Yang Z J, Liao M L. A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai. Gen Mol Res, 2011, 10:2349-2357.
doi: 10.4238/2011.October.5.5
[12] Yang T Z, Zhang X K, Liu H W, Wang Z H. Rht21 in common wheat variety—XN0004 Rht21 in common wheat variety—XN0004. Acta Univ Agric Boreali-Occident, 1993, 21:13-17.
[13] Wu J, Kong X, Wan J, Liu X, Zhang X, Guo X, Zhou R, Zhao G, Jing R, Fu X, Jia J. Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1. Plant Physiol, 2011, 157:2120-2130.
doi: 10.1104/pp.111.185272 pmid: 22010107
[14] Pearce S, Saville R, Vaughan S P, Chandler P M, Wilhelm E P, Sparks C A, Al-Kaff N, Korolev A, Boulton M I, Phillips A L, Hedden P, Nicholson P, Thomas S G. Rht-1 dwarfing genes in hexaploid wheat Rht-1 dwarfing genes in hexaploid wheat. Plant Physiol, 2011, 157:1820-1831.
doi: 10.1104/pp.111.183657
[15] Wu Q, Chen Y, Xie J, Dong L, Wang Z, Lu P, Wang R, Yuan C, Zhang Y, Liu Z. A 36 Mb terminal deletion of chromosome 2BL is responsible for a wheat semi-dwarf mutation. Crop J, 2020, 9:873-881.
doi: 10.1016/j.cj.2020.06.015
[16] Ellis M H, Rebetzke G J, Azanza F, Richards R A, Spielmeyer W. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theor Appl Genet, 2005, 111:423-430.
pmid: 15968526
[17] Bazhenov M S, Divashuk M G, Amagai Y, Watanabe N, Karlov G I. Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker. Mol Breed, 2015, 35:213.
doi: 10.1007/s11032-015-0407-1
[18] Daba S D, Tyagi P, Brown-Guedira G, Mohammadi M. Genome-wide association study in historical and contemporary U.S. winter wheats identifies height-reducing loci. Crop J, 2020, 8:243-251.
doi: 10.1016/j.cj.2019.09.005
[19] Gale M D, Youssefian S. Dwarfing Genes in Wheat. England: Plant Breeding Institute, 1985.
[20] Sun L, Yang W, Li Y, Shan Q, Ye X, Wang D, Yu K, Lu W, Xin P, Pei Z, Guo X, Liu D, Sun J, Zhan K, Chu J, Zhang A. Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line. Plant J, 2019, 97:887-900.
doi: 10.1111/tpj.2019.97.issue-5
[21] Wang Y, Du Y, Yang Z, Chen L, Condon A G, Hu Y G. Rht13 and Rht8 on plant height and some agronomic traits in common wheat Rht13 and Rht8 on plant height and some agronomic traits in common wheat. Field Crops Res, 2015, 179:35-43.
doi: 10.1016/j.fcr.2015.04.010
[22] Vikhe P, Venkatesan S, Chavan A, Tamhankar S, Patil R. Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height. Crop J, 2019, 7:187-197.
doi: 10.1016/j.cj.2018.11.004
[23] Ford B A, Foo E, Sharwood R, Karafiatova M, Vrana J, Macmillan C, Nichols D S, Steuernagel B, Uauy C, Dolezel J, Chandler P M, Spielmeyer W. Rht18 semi-dwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content. Plant Physiol, 2018, 177:168-180.
doi: 10.1104/pp.18.00023
[24] Mo Y, Vanzetti L S, Hale I, Spagnolo E J, Guidobaldi F, Al-Oboudi J, Odle N, Pearce S, Helguera M, Dubcovsky J. Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development. Theor Appl Genet, 2018, 131:2021-2035.
doi: 10.1007/s00122-018-3130-6
[25] Chen S, Gao R, Wang H, Wen M, Xiao J, Bian N, Zhang R, Hu W, Cheng S, Bie T, Wang X. Rht23) regulating panicle morphology and plant architecture in bread wheat Rht23) regulating panicle morphology and plant architecture in bread wheat. Euphytica, 2014, 203:583-594.
doi: 10.1007/s10681-014-1275-1
[26] Würschum T, Langer S M, Longin C F H, Tucker M R, Leiser W L. A modern green revolution gene for reduced height in wheat. Plant J, 2017, 92:892-903.
doi: 10.1111/tpj.13726
[27] Wang M, Wang S, Xia G. From genome to gene: a new epoch for wheat research? Trends Plant Sci, 2015, 20:380-387.
doi: 10.1016/j.tplants.2015.03.010
[28] 陈昊, 谭晓风. 基于第二代测序技术的基因资源挖掘. 植物生理学报, 2014, 50:1089-1095.
Chen H, Tan X F. Excavation of genic resources based on next generation sequencing technologies. Acta Phytophysiol Sin, 2014, 50:1089-1095 (in Chinese with English abstract).
[29] Winfield M O, Wilkinson P A, Allen A M, Barker G L, Coghill J A, Burridge A, Hall A, Brenchley R C, D'amore R, Hall N, Bevan M W, Richmond T, Gerhardt D J, Jeddeloh J A, Edwards K J. Targeted re-sequencing of the allohexaploid wheat exome. Plant Biotechnol J, 2012, 10:733-742.
doi: 10.1111/j.1467-7652.2012.00713.x pmid: 22703335
[30] Paux E, Roger D, Badaeva E, Gay G, Bernard M, Sourdille P, Feuillet C. Characterizing the composition and evolution of homoeologous genomes in hexaploid wheat through BAC-end sequencing on chromosome 3B. Plant J, 2006, 48:463-474.
doi: 10.1111/tpj.2006.48.issue-3
[31] Jordan K W, Wang S, Lun Y, Gardiner L J, Maclachlan R, Hucl P, Wiebe K, Wong D, Forrest K L, Consortium I, Sharpe A G, Sidebottom C H, Hall N, Toomajian C, Close T, Dubcovsky J, Akhunova A, Talbert L, Bansal U K, Bariana H S, Hayden M J, Pozniak C, Jeddeloh J A, Hall A, Akhunov E. A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homoeologous genomes. Genome Biol, 2015, 16:1-18.
doi: 10.1186/s13059-014-0572-2
[32] 许达兴. 小麦茎秆快速发育基因qd1的遗传定位与转录组学分析. 中国农业科学院硕士学位论文, 北京, 2018.
Xu D X. Genetic Mapping of the qd1 Gene of Wheat Stem Quick Development and Transcriptome Analysis in Wheat. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2018 (in Chinese with English abstract).
[33] Robert K, Nicholas B, Ricardo R G, Coghill J A, Archana P, Keywan H P, Cristobal U, Phillips A L. Mutation scanning in wheat by exon capture and next-generation sequencing. PLoS One, 2015, 10:e0137549.
doi: 10.1371/journal.pone.0137549
[34] Hill J T, Demarest B L, Bisgrove B W, Gorsi B, Su Y C, Yost H J. MMAPPR: mutation mapping analysis pipeline for pooled RNA-seq. Genome Res, 2013, 23:687-697.
doi: 10.1101/gr.146936.112
[35] Lincoln S E, Daly M J, Lander E. Constructing Genetic Linkage Maps with MAPMAKER/EXP version 3.0: a Tutorial and Reference Manual, Technical Report, 3rd edn. USA: Whitehead Institute for Biomedical Research, 1993.
[36] Xiong H C, Li Y T, Guo H J, Xie Y D, Zhao L S, Gu J Y, Zhao S R, Ding Y P, Liu L X. Genetic mapping by integration of 55K SNP array and KASP markers reveals candidate genes for important agronomic traits in hexaploid wheat. Front Plant Sci, 2021, 12:628478.
doi: 10.3389/fpls.2021.628478
[37] 张在宝, 李婉杰, 李九丽, 张弛, 胡梦辉, 程琳, 袁红雨. 植物RNA结合蛋白研究进展. 中国农业科学, 2018, 15:4007-4019.
Zhang Z B, Li W J, Li J L, Zhang C, Hu M H, Cheng L, Yuan H Y. The research progress of plant RNA binding proteins. Sci Agric Sin, 2018, 15:4007-4019 (in Chinese with English abstract).
[38] 王天一, 王应祥, 尤辰江. 植物PHD结构域蛋白的结构与功能特性. 遗传, 2021, 43:323-339.
Wang T Y, Wang Y X, You C J. Structural and functional characteristics of plant PHD domain-containing proteins. Hereditas, 2021, 43:323-339 (in Chinese with English abstract).
[39] Hofmann K. A superfamily of membrane-bound O-acyltransferases with implications for Wnt signaling. Trends Biochem Sci, 2000, 25:111-112.
pmid: 10694878
[40] 马小凤, 刘子金, 郑超星, 王星, 武宇, 李洪杰, 张根发. 植物烯醇化酶基因ENO2的功能研究进展. 植物遗传资源学报, 2018, 19:1030-1037.
Ma X F, Liu Z J, Zheng C X, Wang X, Wu Y, Li H J, Zhang G F. Status and progress on functions of plant enolase gene ENO2. J Plant Genet Resour, 2018, 19:1030-1037 (in Chinese with English abstract).
[41] 钟明志, 魏淑红, 彭正松, 杨在君. 小麦Rht矮秆基因研究和应用综述. 分子植物育种, 2018, 16:6670-6677.
Zhong M Z, Wei S H, Peng Z S, Yang Z J. A review of the research and application of Rht dwarf genes in wheat. Mol Plant Breed, 2018, 16:6670-6677 (in Chinese with English abstract).
[42] Worland A J, Sayers E J, Korzun V. Rht8 locus and its significance in international breeding programs Rht8 locus and its significance in international breeding programs. Euphytica, 2001, 119:155-159.
[43] Kowalski A M, Gooding M, Ferrante A, Slafer G A, Orford S, Gasperini D, Griffiths S. Rht8 in contrasting nitrogen treatments and water regimes Rht8 in contrasting nitrogen treatments and water regimes. Field Crops Res, 2016, 191:150-160.
doi: 10.1016/j.fcr.2016.02.026
[44] Tian X, Wen W, Xie L, Fu L, Xu D, Fu C, Wang D, Chen X, Xia X, Chen Q, He Z, Cao S. Rht24 in bread wheat Rht24 in bread wheat. Front Plant Sci, 2017, 8:1379.
doi: 10.3389/fpls.2017.01379
[45] Korzun V, Roder M S, Ganal M W, Worland A J, Law C N. Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I: Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat(Triticum aestivum L.). Theor Appl Genet, 1998, 96:1104-1109.
doi: 10.1007/s001220050845
[46] Chai L, Chen Z, Bian R, Zhai H, Cheng X, Peng H, Yao Y, Hu Z, Xin M, Guo W, Sun Q, Zhao A, Ni Z. Triticum aestivum L.) Triticum aestivum L.). Theor Appl Genet, 2019, 132:1815-1831.
doi: 10.1007/s00122-019-03318-z
[47] Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan A S, Raghothama K G, Baek D, Koo Y D, Jin J B, Bressan R A, Yun D J, Hasegawa P M. Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci USA, 2005, 102:7760-7765.
doi: 10.1073/pnas.0500778102
[48] 唐娜, 姜莹, 何蓓如, 胡银岗. 赤霉素敏感性不同矮秆基因对小麦胚芽鞘长度和株高的效应. 中国农业科学, 2009, 42:3774-3784.
Tang N, Jiang Y, He B R, Hu Y G. Effects of dwarfing genes of Rht-B1b, Rht-D1b and Rht8 with different response to GA3 on coleoptile length and plant height of wheat. Sci Agric Sin, 2009, 42:3774-3784 (in Chinese with English abstract).
[49] Hedden P, Sponsel V. A century of gibberellin research. J Plant Growth Regul, 2015, 34:740-760.
doi: 10.1007/s00344-015-9546-1
[50] Gasperini D, Greenland A, Hedden P, Dreos R, Harwood W, Griffiths S. Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. J Exp Bot, 2012, 63:4419-4436.
doi: 10.1093/jxb/ers138 pmid: 22791821
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] YU Chun-Miao, ZHANG Yong, WANG Hao-Rang, YANG Xing-Yong, DONG Quan-Zhong, XUE Hong, ZHANG Ming-Ming, LI Wei-Wei, WANG Lei, HU Kai-Feng, GU Yong-Zhe, QIU Li-Juan. Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height [J]. Acta Agronomica Sinica, 2022, 48(5): 1091-1102.
[4] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[5] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[6] WANG Juan, ZHANG Yan-Wei, JIAO Zhu-Jin, LIU Pan-Pan, CHANG Wei. Identification of QTLs and candidate genes for 100-seed weight trait using PyBSASeq algorithm in soybean [J]. Acta Agronomica Sinica, 2022, 48(3): 635-643.
[7] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[8] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[9] MA Hong-Bo, LIU Dong-Tao, FENG Guo-Hua, WANG Jing, ZHU Xue-Cheng, ZHANG Hui-Yun, LIU Jing, LIU Li-Wei, YI Yuan. Application of Fhb1 gene in wheat breeding programs for the Yellow-Huai Rivers valley winter wheat zone of China [J]. Acta Agronomica Sinica, 2022, 48(3): 747-758.
[10] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[11] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[12] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[13] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[14] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[15] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!