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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (9): 2339-2350.doi: 10.3724/SP.J.1006.2022.11065


Responses of photosynthetic characteristics and gene expression in different wheat cultivars to elevated ozone concentration at grain filling stage

CAO Ji-Ling1(), ZENG Qing2,*(), ZHU Jian-Guo2,*()   

  1. 1. Key Laboratory of Poyang Lake Basin Agricultural Resource and Ecology of Jiangxi Province / College of Land Resource and Environment, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China
    2. State Key Laboratory of Soil and Sustainable Agriculture / Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, Jiangsu, China
  • Received:2021-07-22 Accepted:2022-01-05 Online:2022-09-12 Published:2022-05-01
  • Contact: ZENG Qing,ZHU Jian-Guo E-mail:jlcao2008@163.com;qzeng@issas.ac.cn;jgzhu@issas.ac.cn
  • Supported by:
    National Natural Science Foundation of China(31800520)


Elevated ground-level ozone (O3) concentration, caused by anthropogenic activities, possesses species-specific or cultivars-specific impacts on plant growth and quality. In this study, a field experiment was conducted using two wheat (Tritcium aestivum L.) cultivars (Yannong 19 and Yangmai 16) with different sensitivities to O3 with two O3 levels (ambient, +50%) in the Chinese free air O3 concentration enrichment platform. The light-response curve, photosynthetic pigment, soluble sugar contents, and photosynthetic gene expression levels were investigated. Results showed that O3 significantly decreased the apparent quantum yield, maximum net photosynthetic rate, chlorophyll a, chlorophyll b, and carotenoid contents. However, the contents of total soluble sugar, glucose, fructose, and sucrose were significantly increased under elevated O3 concentration. The qRT-PCR revealed that the relative gene expression levels of psaA, psbA, and rbcL were significantly decreased by elevated O3 concentration. There were differences in photosynthetic characteristics between two wheat cultivars. Yannong 19 had larger changes in photosynthetic characteristics and the relative chloroplast gene expression levels of psaA, psbA, and rbcL than that of Yangmai 16. This finding can provide theoretical basis for the differential mechanism of photosynthetic response of different wheat varieties and the breeding of wheat tolerant varieties under ozone pollution environment.

Key words: ozone, wheat, photosynthetic responses, soluble sugar, chloroplast gene expression

Table 1

Primer of chloroplast genes"

Primer name
Forward sequence (5'-3')
Reverse sequence (5'-3')

Table 2

Changes of photo-response parameters in wheat flag leaves at grain filling stage under elevated O3 treatments"

烟农19 YN19 扬麦16 YM16
对照Ambient FACE-O3 对照Ambient FACE-O3
最大净光合速率 55 27.6±0.52 aA 28.9±0.47 aA 28.1±1.26 aA 27.7±1.13 aA
Maximum net photosynthetic rate 65 29.4±0.78 aA 29.7±0.55 aA 30.8±0.93 aA 28.5±0.53 aA
(Pmax, μmol m-2 s-1) 75 21.9±0.19 aB 10.1±0.15 cB 23.3±0.01 aB 17.3±0.68 bB
光饱和点 55 1983±79 aA 1867±100 aA 1864±66 aB 1850±81 aA
Light saturation point 65 2106±98 aA 2001±71 bA 2274±75 aA 2050±92 aA
(LSP, μmol m-2 s-1) 75 1565±105 bB 822±51 cB 1564±82 cB 1299±43 dB
光补偿点 55 52.0±2.3 aB 51.9±2.3 aB 37.3±1.3 bB 34.6±1.3 bB
Light compensation point 65 62.7±2.7 bA 68.0±2.3 abA 68.0±2.3 abA 80.0±4.6 aA
(LCP, μmol m-2 s-1) 75 31.0±0.6 abC 36.6±2.4 aC 25.3±1.3 bcC 21.7±1.2 cC
暗呼吸速率 55 2.39±0.18 aB 2.13±0.02 abB 1.69±0.10 bB 1.65±0.16 bB
Dark respiration rate 65 3.42±0.10 bA 3.79±0.24 abA 4.17±0.18 abA 4.42±0.20 aA
(Rd, μmol m-2 s-1) 75 1.50±0.16 aC 1.29±1.00 aC 1.31±0.16 aB 0.98±0.10 aB
表观量子效率 55 0.054±0.005 aA 0.052±0.004 aA 0.051±0.004 aA 0.056±0.005 aA
Apparent quantum yield 65 0.056±0.004 aA 0.061±0.004 aA 0.058±0.005 aA 0.056±0.002 aA
(AQY) 75 0.042±0.002 abB 0.028±0.005 cB 0.046±0.003 aB 0.034±0.004b cB

Fig. 1

Response curve of photosynthetic rate in wheat flag leaves at grain filling stage under elevated O3 treatments YN19: Yannong 19; YM16: Yangmai 16. 55 d, 65 d, and 75 d indicate days of O3 treatment."

Fig. 2

Changes of photosynthetic pigment contents and composition in wheat flag leaves at grain filling stage under elevated O3 treatments YN19: Yannong 19; YM16: Yangmai 16. 55 d, 65 d, and 75 d indicate days of O3 treatment. Different letters in the bars indicate significant different at P < 0.05"

Table 4

Changes of soluble sugar contents in wheat flag leaves at grain filling stage under elevated O3 treatments"

Suger content
DOT 烟农19 YN19 扬麦16 YM16
对照 Ambient FACE-O3 对照 Ambient FACE-O3
Total soluble sugar
(mg g-1)
55 d 0.616±0.049 aA 0.705±0.058 aA 0.708±0.048 aA 0.726±0.060 aA
65 d 0.511±0.073 aAB 0.522±0.107 aAB 0.551±0.045 aB 0.583±0.068 aB
75 d 0.404±0.032 abB 0.441±0.022 aB 0.373±0.024 bC 0.432±0.022 abC
(mg g-1)
55 d 0.040±0.001 aA 0.043±0.002 aA 0.044±0.002 aA 0.040±0.002 aA
65 d 0.038±0.000 aA 0.036±0.001 abB 0.036±0.002 abB 0.033±0.001 bB
75 d 0.030±0.002 bB 0.035±0.001 aB 0.030±0.000 bC 0.028±0.001 bC
(mg g-1)
55 d 0.585±0.053 aA 0.686±0.116 aA 0.606±0.025 aA 0.630±0.009 aA
65 d 0.396±0.035 bB 0.411±0.035 abB 0.565±0.094 aA 0.464±0.006 abB
75 d 0.177±0.034 bC 0.285±0.043 aB 0.150±0.028 bB 0.214±0.016 abC
(mg g-1)
55 d 0.020±0.008 aB 0.023±0.004 aB 0.020±0.004 aB 0.021±0.003 aC
65 d 0.079±0.002 aA 0.078±0.002 aA 0.066±0.001 bA 0.051±0.002 cB
75 d 0.069±0.002 abA 0.074±0.008 aA 0.060±0.004 bA 0.080±0.004 aA

Fig. 3

Changes of chloroplast gene expression level in wheat flag leaves at grain filling stage under elevated O3 (75 d) treatments YN19: Yannong 19; YM16: Yangmai 16. *, **, and ns representing significant at P < 0.05, P < 0.01, and no significant, respectively."

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