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Acta Agronomica Sinica ›› 2022, Vol. 48 ›› Issue (7): 1697-1708.doi: 10.3724/SP.J.1006.2022.14123

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Functional analysis of GmTGA26 gene under salt stress in soybean

KE Dan-Xia(), HUO Ya-Ya, LIU Yi, LI Jin-Ying, LIU Xiao-Xue   

  1. College of Life Sciences, Xinyang Normal University / Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang 464000, Henan, China
  • Received:2021-07-14 Accepted:2021-10-19 Online:2022-07-12 Published:2021-11-02
  • Contact: KE Dan-Xia E-mail:kdx_029@163.com
  • Supported by:
    National Natural Science Foundation of China(U1904102);Training Plan for Young Backbone Teachers in Colleges and Universities in Henan Province;Nanhu Scholars Program for Young Scholars of Xinyang Normal University

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Abstract:

TGA transcription factors are a subfamily of bZIP, which play important roles in pathogen and abiotic stress responses. A TGA transcription factor family gene GmTGA26 was screened and cloned from soybean in this study. Homologous protein comparison showed that GmTGA26 had a conserved leucine zipper domain and had the highest homology with wild soybean. The analysis of gene expression characteristics revealed that GmTGA26 gene was induced by salt stress in soybean. In addition, GmTGA26 gene encoded nuclear localization protein and had transcriptional activation activity. The “complex” soybean plants overexpressing GmTGA26 were obtained through Agrobacterium rhizogenes-mediated hairy root transformation of soybean. The growth state of “complex” soybean plants was better than the empty vector control under salt stress. Meanwhile, the MDA content and relative plasma membrane permeability decreased significantly (P < 0.05), while the chlorophyll content and root activity increased significantly (P < 0.05). The qRT-PCR results indicated that overexpression of GmTGA26 in soybean hairy roots under salt stress could significantly up-regulate the expression of stress response genes. The above results showed that overexpression of GmTGA26 significantly enhanced the salt tolerance of “complex” soybean plants. It is speculated that GmTGA26 participates in the regulation of soybean salt stress response by regulating a series of downstream stress response genes.

Key words: soybean, TGA transcription factor, hairy root transformation, saline tolerance

Table 1

Primers used for gene expression analysis and vector construction"

引物名称
Primer name		 正向引物
Forward primer (5′-3′)		 反向引物
Reverse primer (5′-3′)
GmTGA26 ATGGCTGACGCCAGTCCTA TCAGTCTCTTGGGCGGG
GmTGA26-OE CGGGATCCATGGCTGACG GGGGTACCTCAGTCTCTTGGGC
GUS GTCGCGCAAGACTGTAACCA CGGCGAAATTCCATACCTG
GmTGA26-qRT GGAGCAACAGTTAGTGGGTATCAC CTTCCATGCCCTGAGACAAAGC
ACT11-qRT GAGCTATGAATTGCCTGATGG CGTTTCATGAATTCCAGTAGC
GmDHN15-qRT TTTTGTTTTGTTGTATTGTGTAG GAAAAATCCTCCACCTGACGA
GmWD40-qRT TGCCAGTCTCGTTAGGCTTTTC CTTATTGAGTTGTTGTTTGGCAG
GmMYB48-qRT AACAACACTCTTCAGCCAGTTT GGGCAAAACAAACTTTCCTCAT
GmGST1-qRT CACAATGAGCAGCCCATAGCA CTTCAACATTCTTCTCACGCTCT
GmLEA-qRT GGTGGGTGAAACCGCACAAGA ATGGATGCCGCCACTCCGCCAG
GmNHX5-qRT GTCTGGGTTCAGTCTCGCAC ATCAGAAAGAGCAAGCCACCA
GmSOS1-qRT TTGTGCTGCATTTCTTCGAG CGTGCTTCTTCTCCTTCCAC

Fig. 1

Sequence alignment and phylogenetic tree analysis of GmTGA26 and its homologous proteins A: the conserved BRLZ (basic region leucin zipper) domain alignment analysis of GmTGA26 and its homologous proteins in some other plants; B: phylogenetic tree of GmTGA26 and its homologs. The scale represents genetic distance, indicating the proximity relationships among species."

Fig. 2

Relative expression analysis of GmTGA26 gene under different abiotic stresses treatments * and ** mean significant difference at the 0.05 and 0.01 probability levels, respectively."

Fig. 3

Subcellular localization and transcription activation analysis of GmTGA26 A: co-localization of GmTGA26; the recombinant plasmids of GmTGA26-GFP and NtTGA2.2-RFP were co-transformed into Arabidopsis protoplasts, and 35S::GFP and NtTGA2.2-RFP were used as positive control; bar: 10 µm; B: transcriptional activity analysis of GmTGA26 in yeast cells. DDO: double dropout medium (SD/-Leu/-Trp); DDO/X/A: double dropout medium with 125 ng mL-1 Aureobasidin A and 40 μg mL-1 X-α-gal; QDO: quadruple dropout medium (SD/-Ade/-His/-Leu/-Trp); BD-53+AD-T: positive interaction control; BD-Lam+AD-T: negative control."

Fig. 4

Positive hairy roots identification of transgenic soybean composite plants A: GUS staining of negative hairy root; B: GUS staining of positive hairy root; C: detection of GUS gene in positive hairy roots by PCR. Bar: 200 μm; M: 1 kb DNA ladder; 1-7: PCR amplification of GUS gene."

Fig. 5

Performance of GmTGA26 transgenic composite soybean plants under salt stress A: growth status of plants treated with different concentrations of salt for one week; bar: 2 cm; B: relative GmTGA26 expression level in hairy roots by qRT-PCR, and ACTIN11 gene was used as internal reference; C-E: total root length, shoot and root dry weight were measured one week after treatments. EV: empty vector; OE: overexpressing GmTGA26 plants. Values are presented as means of three independent replicates (n = 20); the error bars indicate SDs; * and ** mean significant difference at the 0.05 and 0.01probability levels, respectively."

Fig. 6

Physiological characteristics of transgenic soybean under salt stress EV: empty vector; OE: overexpressing GmTGA26 plants. * and ** mean significant difference at the 0.05 and 0.01 probability levels, respectively."

Fig. 7

Relative expression levels of five stress-responsive genes in soybean hairy roots under the normal and 100 mmol L-1 NaCl treatments EV: empty vector; OE: overexpressing GmTGA26 plants. * and ** mean significant difference at the 0.05 and 0.01 probability levels, respectively."

Fig. 8

Relative expression levels of two salt stress-responsive genes in soybean hairy roots under the normal and 100 mmol L-1 NaCl treatments EV: empty vector; OE: overexpressing GmTGA26 plants. * and ** mean significant difference at the 0.05 and 0.01 probability levels, respectively."

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