亚麻PLA1基因家族的鉴定及表达分析

doi:10.3724/SP.J.1006.2023.24224

Abstract

Abstract:

Phospholipase A1 (PLA1) plays an important role in plant growth and development and stress responses. However, there is no function study of PLA1 in flax. In this study, we identified the PLA1 gene family in seven species including flax by bioinformatics, and analyzed the sequence characteristics, phylogenetic evolution, cis-acting elements, collinearity, and replication events of PLA1 (LuPLA1) genes in flax. Transcriptome data were used to analyze the relative expression patterns in different genetic backgrounds and organs, and qRT-PCR was used to analyze the relative expression patterns in different tissues and developmental stages, and under different stress treatments. The results showed that there were 14, 21, 15, 20, 41, 18, and 35 PLA1 members identified in flax, Arabidopsis, maize, rice, soybean, castor, and cassava, respectively. LuPLA1 were distributed on 8 chromosomes. Sequence analysis showed that except for LuPLA1-7 and LuPLA1-10, all the other members had introns and most of them had one intron, with protein length of 388-1759 aa, isoelectric point of 5.99-9.19, and molecular weight of 41.55-192.61 kD. All the LuPLA1 proteins were hydrophilic, and most of them localized in vacuoles and contained 4-25 motifs. Phylogenetic analysis revealed that the PLA1 proteins were divided into four clades, and clade IV had the largest number of PLA1 members. Collinearity analysis indicated that LuPLA1 had homologous genes in Arabidopsis, maize, rice, cassava, and soybean, and had the most homologous genes with soybean and cassava. There were two tandem duplicated gene pairs and seven segment duplicated genes pairs of LuPLA1 family members, and all the duplicated genes underwent purification selection. Transcriptome analysis showed that most LuPLA1 members exhibited organ-specific expression patterns. The promoter regions of LuPLA1 contained a large number of hormone and stress response elements. The qRT-PCR further confirmed that the relative expression of LuPLA1 genes were induced by hormone, drought, high salt, low temperature, and high temperature. LuPLA1-1 was induced by IAA. LuPLA1-1 and LuPLA1-6 were induced by NAA. LuPLA1-1, LuPLA1-5, and LuPLA1-6 were induced by GA₃. LuPLA1-12/14 was induced by NaCl and PEG. Except for LuPLA1-2/4, the other genes were induced by high temperature. All LuPLA1 members were induced by low temperature, and the response to low temperature was the most obvious. This study laid a foundation for further analysis of the function of LuPLA1 gene family.

Key words: flax, LuPLA1, gene family, the relative expression pattern

ZHAO Li-Rong, LI Wen, WANG Li-Min, QI Yan-Ni, LI Wen-Juan, XIE Ya-Ping, DANG Zhao, ZHAO Wei, ZHANG Jian-Ping. Identification and relative expression pattern of PLA1 gene family in flax[J].Acta Agronomica Sinica, 2023, 49(11): 2949-3295.

Figures/Tables 16

Table S1

Table S2

Fig. 1

Table 1

Fig. 2

Table 2

Fig. S1

Fig. S2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. S3

Fig. 7

Fig. 8

Fig. 9

References 33

[1]	Huis R, Hawkins S, Neutelings G. Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.). BMC Plant Biol, 2010, 10: 71. doi: 10.1186/1471-2229-10-71
[2]	党照, 张建平, 王利民, 李闻娟, 齐燕妮, 谢亚萍, 赵玮. 胡麻新品种陇亚15号选育技术报告. 中国麻业科学, 2020, 42(4): 145-149.
	Dang Z, Zhang J P, Wang L M, Li W J, Qi Y N, Xie Y P, Zhao W. Technical report on breeding of a new flax variety Longya 15. Plant Fiber Sci China, 2020, 42(4): 145-149 (in Chinese with English abstract).
[3]	米智, 刘荔贞, 李慧, 张弘驰. 响应面法优化胡麻籽饼粕黄酮提取工艺及抗氧化活性的研究. 中国粮油学报, 2022, 37(3): 1-11.
	Mi Z, Liu L Z, Li H, Zhang H C. Optimization of flavonoids extraction and antioxidant activity of flax seed cake by response surface method. J Chin Cereals Oils Assoc, 2022, 37(3): 1-11 (in Chinese with English abstract).
[4]	赵利, 王斌, 苗红梅, 马琴. 胡麻种质资源籽粒表型与品质性状评价及其相关性研究. 植物遗传资源学报, 2020, 21: 243-251. doi: 10.13430/j.cnki.jpgr.20191107001
	Zhao L, Wang B, Miao H M, Ma Q. Evaluation and correlation study on grain phenotype and quality traits of flax germplasm resources. J Plant Genet Resour, 2020, 21: 243-251 (in Chinese with English abstract).
[5]	杨阳, 王晶懋. 百合响应非生物胁迫的分子机制研究. 分子植物育种, 2018, 16: 5046-5054.
	Yang Y, Wang J M. Molecular mechanism of lily response to abiotic stress. Mol Plant Breed, 2018, 16: 5046-5054 (in Chinese with English abstract).
[6]	Gilles L M, Khaled A, Laffaire J B, Chaignon S, Gendrot G, Laplaige J, Bergès H, Beydon G, Bayle V, Barret P, Comadran J, Martinant J P, Rogowsky P M, Widiez T. Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize. EMBO J, 2017, 36: 707-717. doi: 10.15252/embj.201796603 pmid: 28228439
[7]	葛金涛, 王江英, 汤雪燕, 腾年军, 朱朋波, 孙明伟, 赵统利, 邵小斌. 百合叶片磷脂酶基因家族转录组学分析. 江苏农业科学, 2022, 50(3): 36-42.
	Ge J T, Wang J Y, Tang X Y, Teng N J, Zhu P B, Sun M W, Zhao T L, Shao X B. Transcriptomic analysis of the phospholipase gene family in lily leaves. Jiangsu Agric Sci, 2022, 50(3): 36-42 (in Chinese with English abstract).
[8]	Chapman K D. Phospholipase activity during plant growth and development and in response to environmental stress. Trends Plant Sci, 1998, 3: 419-426. doi: 10.1016/S1360-1385(98)01326-0
[9]	Scherer G F E. Activation of phospholipase A2, by auxin and mastoparan in hypocotyl segments from zucchini and sunflower. J Plant Physiol, 1995, 145: 483-490. doi: 10.1016/S0176-1617(11)81775-X
[10]	Günther F, Scherer E, Arnold B. Inhibitors of animal phospholipase A2 enzymes are selective inhibitors of auxin-dependent growth. Implications for auxin-induced signal transduction. Planta, 1997, 202: 462-469. doi: 10.1007/s004250050150
[11]	Fan L, Wang Z X. Antisense suppression of phospholipase Dα retards abscisic acid- and ethylene-promoted senescence of postharvest Arabidopsis leaves. Plant Cell, 1997, 9: 2183-2196. doi: 10.1105/tpc.9.12.2183 pmid: 9437863
[12]	苏燕南. 磷脂酶A1基因的克隆和原核表达. 安徽工程大学硕士学位论文,安徽芜湖, 2013.
	Su Y N. Cloning and Prokaryotic Expression of Phospholipase A1 Gene. MS Thesis of Anhui Polytechnic University, Wuhu, Anhui, China, 2013 (in Chinese with English abstract).
[13]	程实. 非钙离子依赖型磷脂酶A1的异源表达及制备. 江南大学博士学位论文,江苏无锡, 2021.
	Cheng S. Heterologous Expression and Preparation of Non Calcium Ion Dependent Phospholipase A1. PhD Dissertation of Jiangnan University, Wuxi, Jiangsu, China, 2021 (in Chinese with English abstract).
[14]	安炎黄. 磷脂酶参与冬凌草甲素对拟南芥的化感潜能作用. 西北师范大学硕士学位论文,甘肃兰州, 2019.
	An Y H. Phospholipase Involved in the Allelopathic Effect of Rubescens on Arabidopsis thaliana. MS Thesis of Northwest Normal University, Lanzhou, Gansu, China, 2019 (in Chinese with English abstract).
[15]	Kenji M, Sachiko F, Miho I, Tadahiko K. A tomato lipase homologous to DAD1 (LeLID1) is induced in post-germinative growing stage and encodes a triacylglycerol lipase. FEBS Lett, 2004, 569: 195-200. doi: 10.1016/j.febslet.2004.05.064 pmid: 15225633
[16]	Nishihara M, Kamata M, Koyama T, Yazawa K. New Phospholipase A1-producing bacteria from a marine fish. Mar Biotechnol, 2008, 10: 382-387. doi: 10.1007/s10126-007-9074-5 pmid: 18293038
[17]	Tavernier E, Pugin A. Phospholipase activities associated with the tonoplast from Acer pseudoplatanus cells: identification of a phospholipase A1 activity. Biochim Biophys Acta, 1995, 1233: 118-122. pmid: 7865536
[18]	Ishiguro S, Kawai-Oda A, Ueda J, Nishida I, Okada K. The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis. Plant Cell, 2001, 13: 2191-2209. doi: 10.1105/tpc.010192 pmid: 11595796
[19]	Noiriel A, Benveniste P, Banas A, Stymne S, Bouvier-Navé P. Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis thaliana. Eur J Biochem, 2004, 271: 3752-3764. doi: 10.1111/ejb.2004.271.issue-18
[20]	Seo Y S, Eun Y K, Hyung G M, Woo T K. Heterologous expression, and biochemical and cellular characterization of CaPLA1 encoding a hot pepper phospholipase A1 homolog. Plant J Cell Mol Biol, 2008, 53: 895-908. doi: 10.1111/j.1365-313X.2007.03380.x
[21]	姜惠娜, 敬松, 李晗玉, 佘木子, 张新飞, 呼天明, 付娟娟, 苗彦军. 西藏野生垂穗披碱草EnPLA1基因克隆与表达分析. 草地学报, 2021, 29: 2141-2148. doi: 10.11733/j.issn.1007-0435.2021.10.004
	Jiang H N, Jing S, Li H Y, She M Z, Zhang X F, Hu T M, Fu J J, Miao Y J. Cloning and expression analysis of EnPLA1 gene in wild Flagellae from Xizang. Acta Agrest Sin, 2021, 29: 2141-2148 (in Chinese with English abstract).
[22]	Hyun Y, Choi S, Hwang H J, Yu J, Nam S J, Ko J, Park J Y, Seo Y S, Kim E Y, Ryu S B, Kim W O, Lee Y H, Kang H, Lee I. Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis. Dev Cell, 2008, 14: 183-192. doi: 10.1016/j.devcel.2007.11.010 pmid: 18267087
[23]	Liu C X, Li X, Meng D X, Zhong Y, Chen C, Dong X, Xu X W, Chen B J, Li W, Li L, Tian X L, Zhao H M, Song W B, Luo H S, Zhang Q H, Lai J S, Jin W W, Yan J B, Chen S J. A 4-bp insertion at ZmPLA1encoding a putative phospholipase a generates haploid induction in maize. Mol Plant, 2017, 10: 520-522. doi: 10.1016/j.molp.2017.01.011
[24]	文钦, 贾思思, 王加峰, 黄翠红, 王慧, 陈志强, 郭涛. 水稻单倍体诱导基因OsMATL突变体的创制与分析. 作物学报, 2021, 47: 816-825.
	Wen Q, Jia S S, Wang J F, Huang C H, Wang H, Chen Z Q, Guo T. Creation and analysis of OsMATL mutant of rice haploid inducible gene. Acta Agron Sin, 2021, 47: 816-825 (in Chinese with English abstract).
[25]	Liu H Y, Wang K, Jia Z M, Gong Q, Lin Z S, Du L P, Pei X W, Ye X G. Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system. J Exp Bot, 2020, 71: 1337-1349. doi: 10.1093/jxb/erz529
[26]	Lynch M, Conery J S. The evolutionary fate and consequences of duplicate genes. Science, 2000, 290: 1151-1155. doi: 10.1126/science.290.5494.1151 pmid: 11073452
[27]	张丹丹, 杨瑶君, 江纳, 付春. 黄藤Dof家族的全基因组鉴定及系统进化分析. 西南林业大学学报(自然科学), 2021, 41(6): 126-138.
	Zhang D D, Yang Y J, Jiang N, Fu C. Genome-wide identification and phylogenetic analysis of the Dof family of Rattan japonica. J Southwest For Univ (Nat Sci), 2021, 41(6): 126-138 (in Chinese with English abstract).
[28]	刘艳丽, 周媛, 曹丹, 马林龙, 龚自明, 金孝芳. 基于茶蛋白质组学数据分析植物亚细胞定位预测软件的应用. 植物科学学报, 2020, 38: 671-677.
	Liu Y L, Zhou Y, Cao D, Ma L L, Gong Z M, Jin X F. Application of plant subcellular localization prediction software based on tea proteomic data analysis. Plant Sci J, 2020, 38: 671-677 (in Chinese with English abstract).
[29]	冷非凡, 罗文, 李渊利, 孙尚琛, 王永刚. 嗜铁钩端螺旋菌中铁硫簇相关蛋白的生物信息学分析. 基因组学与应用生物学, 2018, 37: 5296-5303.
	Leng F F, Luo W, Li Y L, Sun S C, Wang Y G. Bioinformatics analysis of iron-sulfur cluster related proteins in Helicobacter ferrophila. Genomics Appl Biol, 2018, 37: 5296-5303 (in Chinese with English abstract).
[30]	唐永凯, 贾永义. 荧光定量PCR数据处理方法的探讨. 生物技术, 2008, (3): 89-91.
	Tang Y K, Jia Y Y. Discussion on data processing methods of fluorescence quantitative PCR. Biotechnology, 2008, (3): 89-91 (in Chinese with English abstract).
[31]	张群, 贾倩茹, 章文华. 磷脂介导的生长素信号转导研究进展. 南京农业大学学报, 2020, 43(2): 195-203.
	Zhang Q, Jia Q R, Zhang W H. Research progress in phospholipid-mediated auxin signal transduction. J Nanjing Agric Univ, 2020, 43(2): 195-203 (in Chinese with English abstract).
[32]	Kelliher T, Starr D, Richbourg L, Chintamanani S, Delzer B, Nuccio M L, Green J, Chen Z Y, McCuiston J, Wang W L, Liebler T, Bullock P, Martin B. MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction. Nature, 2017, 7639: 105-109.
[33]	Zhu S H, Wang X Y, Chen W, Yao J B, Li Y, Fang S T, Lyu Y J, Li X X, Pan J W, Liu C Y, Li Q L, Zhang Y S. Cotton DMP gene family: characterization, evolution, and expression profiles during development and stress. Int J Biol Macromol, 2021, 183: 1257-1269. doi: 10.1016/j.ijbiomac.2021.05.023 pmid: 33965485

Related Articles 15

[1]	Hu Zhao, Qian Run, Xie Feng-Pu, Ying Su-Ping. Genome-wide identification and expression analysis of the SPX gene family in rice under phosphorus treatment [J]. Acta Agronomica Sinica, 2026, 52(6): 1902-1912.
[2]	Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[3]	Sun Shu-Feng, Xu Zhen-Nan, Huang Jia-Xin, Weng Jian-Feng, Li Xin-Hai. Genome-wide identification of the maize MAPK gene family and its response to Fusarium verticillioides infection [J]. Acta Agronomica Sinica, 2026, 52(5): 1291-1308.
[4]	Song Yu-Zhen, Bheel Chander Kumar, Wang Yue, Zhang Ying-Xing, Guo Juan, Khound Rituraj, Santra Dipak Kumar, Cao Xiao-Ning, Wang Rui-Yun. Genome-wide identification of the AP2 subfamily in broomcorn millet and functional characterization of PmAP2-1 and PmAP2-9 in salt tolerance [J]. Acta Agronomica Sinica, 2026, 52(4): 1127-1139.
[5]	Niu Li, Wang Yong-Sheng, Wang Chang-Jie, Zhang Hong, Meng Ya-Xiong, Li Bao-Chun, Yang Ke, Ma Xiao-Le, Yao Li-Rong, Si Er-Jing, Wang Hua-Jun, Wang Jun-Cheng. Identification and analysis of the NAC gene family in barley (Hordeum vulgare L.) and functional validation of HvNAC38 in salt tolerance [J]. Acta Agronomica Sinica, 2026, 52(3): 688-707.
[6]	Yang Zong-Tao, Yang Ting, Wang Yu-Tong, Ai Jing, Li Yan-Ye, Liu Jia-Yong, Deng Jun, Zhao Yong, Zhang Yue-Bin. Identification and expression analysis of the CLC gene family in sugarcane [J]. Acta Agronomica Sinica, 2026, 52(3): 722-734.
[7]	Meng Cheng, Wang Zhe. Genome-wide identification and expression analysis of the ZmPFK gene family under biotic and abiotic stresses in maize [J]. Acta Agronomica Sinica, 2026, 52(3): 764-779.
[8]	Hu Cheng-Zhen, Gao Wei-Dong, Kong Bin-Xue, Wang Jian-Fei, Che Zhuo, Yang De-Long, Chen Tao. Genome-wide identification of the TaAPC11 gene family in wheat and functional characterization of TaAPC11-5B in drought stress responses [J]. Acta Agronomica Sinica, 2026, 52(1): 148-164.
[9]	WANG Bin, MENG Jiang-Yu, QIU Hao-Liang, HE Ya-Jun, QIAN Wei. Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(8): 2100-2110.
[10]	LU Wen-Jia, WANG Jun-Cheng, YAO Li-Rong, ZHANG Hong, SI Er-Jing, YANG Ke, MENG Ya-Xiong, LI Bao-Chun, MA Xiao-Le, WANG Hua-Jun. Genome-wide identification of PRX gene family and analysis of their expressions under drought stress in barley [J]. Acta Agronomica Sinica, 2025, 51(5): 1198-1214.
[11]	ZHOU En-Qiang, MIAO Ya-Mei, ZHOU Yao, YAO Meng-Nan, ZHAO Na, WANG Yong-Qiang, ZHU Yu-Xiang, XUE Dong, LI Zong-Di, SHI Yu-Xin, LI Bo, WANG Kai-Hua, GU Chun-Yan, WANG Xue-Jun, WEI Li-Bin. Analysis of bZIP gene family and identification of seed development candidate genes in pea based on seed development transcriptome [J]. Acta Agronomica Sinica, 2025, 51(4): 914-931.
[12]	GUO Bing, QIN Jia-Fan, LI Na, SONG Meng-Yao, WANG Li-Ming, LI Jun-Xia, MA Xiao-Qian. Genome-wide identification and expression analysis of SHMT gene family in foxtail millet (Setaria italica L.) [J]. Acta Agronomica Sinica, 2025, 51(3): 586-5897.
[13]	QI Jia-Min, XU Chun-Miao, XIAO Bin. Genome-wide identification and expression analysis of TIFY gene family in potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2024, 50(9): 2297-2309.
[14]	YANG Yu-Chen, JIN Ya-Rong, LUO Jin-Chan, ZHU Xin, LI Wei-Hang, JIA Ji-Yuan, WANG Xiao-Shan, HUANG De-Jun, HUANG Lin-Kai. Identification and expression analysis of the WD40 gene family in pearl millet [J]. Acta Agronomica Sinica, 2024, 50(9): 2219-2236.
[15]	HE Dan-Dan, SHU Ya-Zhou, ZHOU Hai-Lian, WU Song-Guo, WEI Xiao-Shuang, YANG Ming-Chong, LI Bo, WU Zheng-Dan, HAN Shi-Jian, YANG Juan, WANG Ji-Bin, WANG Ling-Qiang. OsRPTA18 participated in the regulation of leaf inclination in rice [J]. Acta Agronomica Sinica, 2024, 50(8): 1934-1947.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

基因名称 Gene name	上游引物 Forward primer (5′-3′)	下游引物 Reverse primer (5′-3′)
GAPDH	CTTTACCCTCAGCAAATCCG	AGGTTCTTCCCGCTCTCAAT
PLA1-1	TCTTCCCTCAGAACAGAGGTGTATTT	AGTCTTGTTGGACCCTGTCCCTAG
PLA1-2/4	CCTCTTCTCGTCCCTTGCTTTGA	GGAGAAGAGCAGGGAACGAAACT
PLA1-3/11	ATTTCCGTTTATGGGAGGTTTGCC	TAAAGGCAAATACCCTCCAAACGG
PLA1-5	CTTGATGGGGAGAATGTGAGGA	GTTGGAGCTGCGGAAGTTGATA
PLA1-6	GCTTGATGGGAAGGATGTGAGGAT	AGAGGGCGTTTGTTGTCATTTGGT
PLA1-7/10	CGAATTGACCCTAAAGGACACG	GCTTAACTGGGATTTCCTGTGC
PLA1-8/9	GAACAGCGACGAATAATCCAACG	CTTGTCGCTGCTTATTAGGTTGC
PLA1-12/14	ATGGGAGGTTTGCCGGGCTAC	TACCCTCCAAACGGCCCGATG

名称 Name	登录号 Accession number	名称 Name	登录号 Accession number
Longya-10-fruit-1	SRR8281918	Heiya-14-fruit-1	SRR8281912
Longya-10-fruit-2	SRR8281919	Heiya-14-fruit-2	SRR8281913
Longya-10-stem-1	SRR8281920	Heiya-14-stem-1	SRR8281916
Longya-10-stemt-2	SRR8281921	Heiya-14-stem-2	SRR8281917

基因对 Gene pairs	复制事件 Duplication events	同义替换率 K_s	非同义替换率 K_a	K_a/K_s	选择类型 Selection type	分歧时间 Divergence time (Mya)
LuPLA1-7/LuPLA1-8	串联复制 Tandem duplication	0.5411	0.1419	0.2622	纯化选择 Purifying selection	44.3525
LuPLA1-9/LuPLA1-10	串联复制 Tandem duplication	0.5093	0.1448	0.2843	纯化选择 Purifying selection	41.7459
LuPLA1-2/LuPLA1-4	片段复制 Segmental duplication	0.1189	0.0213	0.1791	纯化选择 Purifying selection	9.7459
LuPLA1-3/LuPLA1-11	片段复制 Segmental duplication	0.1217	0.0061	0.0501	纯化选择 Purifying selection	9.9754
LuPLA1-3/LuPLA1-12	片段复制 Segmental duplication	1.0065	0.0692	0.0687	纯化选择 Purifying selection	82.5000
LuPLA1-7/LuPLA1-9	片段复制 Segmental duplication	0.5776	0.1400	0.2424	纯化选择 Purifying selection	47.3443
LuPLA1-11/LuPLA1-12	片段复制 Segmental duplication	0.9443	0.0758	0.0803	纯化选择 Purifying selection	77.4016
LuPLA1-11/LuPLA1-14	片段复制 Segmental duplication	0.9403	0.0770	0.0819	纯化选择 Purifying selection	77.0738
LuPLA1-12/LuPLA1-14	片段复制 Segmental duplication	0.0845	0.0135	0.1598	纯化选择 Purifying selection	6.9262

基因名称 Gene name	基因号 Gene ID	染色体位置 Chromosome location	外显子数量 No. of exons	蛋白序列长度 Protein length (aa)	分子量 Molecular weight (kD)	等电点 Isoelectric point	亚细胞定位 Subcellular location
LuPLA1-1	L.us.o.m.scaffold73.66	Chr1:21780419- 21783081(-)	7	459	50.96	5.99	液泡 Vacuole
LuPLA1-2	L.us.o.m.scaffold17.61	Chr5:5454111- 5456030(-)	2	388	41.55	8.08	液泡 Vacuole
LuPLA1-3	L.us.o.m.scaffold255.68	Chr5:17092681- 17095829(-)	2	463	50.92	8.00	液泡 Vacuole
LuPLA1-4	L.us.o.m.scaffold11.49	Chr6:4217828- 4219482(+)	2	390	41.81	7.52	液泡 Vacuole
LuPLA1-5	L.us.o.m.scaffold100.2	Chr7:7572800- 7576425(+)	7	400	44.50	6.97	液泡 Vacuole
LuPLA1-6	L.us.o.m.scaffold284.7	Chr7:7630026- 7633875(+)	7	391	43.23	6.92	液泡 Vacuole
LuPLA1-7	L.us.o.m.scaffold219.29	Chr8:17228540- 17230314(+)	1	483	52.59	9.19	细胞膜, 液泡 Cell membrane, vacuole
LuPLA1-8	L.us.o.m.scaffold219.27	Chr8:17242561- 17243859(+)	2	421	45.31	8.99	液泡 Vacuole
LuPLA1-9	L.us.o.m.scaffold253.44	Chr11:1866126- 1867424(-)	2	421	45.30	8.99	液泡 Vacuole
LuPLA1-10	L.us.o.m.scaffold253.45	Chr11:1884093- 1885373(-)	1	426	45.90	9.15	叶绿体, 液泡 Chloroplast, vacuole
LuPLA1-11	L.us.o.m.scaffold144.142	Chr11:13906282- 13908918(-)	2	463	50.94	7.55	叶绿体, 液泡 Chloroplast, vacuole
LuPLA1-12	L.us.o.m.scaffold263.24	Chr12:5589259- 5592930(+)	2	453	49.49	7.01	液泡 Vacuole
LuPLA1-13	L.us.o.m.scaffold220.83	Chr15:8920678- 8929774(-)	28	1759	192.61	6.80	液泡 Vacuole
LuPLA1-14	L.us.o.m.scaffold0.620	Chr15:11287238- 11289213(-)	2	452	49.43	6.97	叶绿体, 液泡 Chloroplast, vacuole

Identification and relative expression pattern of PLA1 gene family in flax

RichHTML416

PDF