大豆不同生育时期耐盐性综合评价及耐盐种质筛选

doi:10.3724/SP.J.1006.2025.55013

Abstract

Abstract:

Soil salinization is a major abiotic stress that severely hampers soybean growth and productivity. The evaluation and selection of salt-tolerant soybean germplasm are essential for identifying salt-alkali tolerance genes, breeding salt-tolerant cultivars, and improving the efficient use of saline-alkali soils. In this study, 50 soybean germplasm accessions were evaluated for salt tolerance at three developmental stages: germination (0.6% NaCl), seedling (1.5% NaCl), and the full growth period (0.9% NaCl). Salt tolerance was assessed using germination rate at the germination stage; at the seedling stage, ten physiological and morphological indicators were measured, including plant height, leaf area, SPAD value, fresh and dry weights of shoots and roots, and malondialdehyde content. During the full growth stage, eight agronomic traits were evaluated, such as plant height, pod height, number of effective branches, pods per plant, seeds per plant, and seed weight per plant. A comprehensive evaluation was conducted using correlation analysis, principal component analysis (PCA), membership function analysis, and cluster analysis. Stepwise regression was employed to construct predictive models for salt tolerance at the seedling and full-growth stages, identifying key evaluation indicators for each stage. Based on the salt injury index, 8 highly salt-tolerant, 10 salt-tolerant, and 6 highly salt-sensitive germplasms were identified at the germination stage. PCA combined with the membership function approach identified 12 salt-tolerant accessions at the seedling stage. Cluster analysis grouped the 50 accessions into five categories during the full growth stage: 3 highly salt-tolerant, 3 salt-tolerant, 20 moderately salt-tolerant, 19 salt-sensitive, and 5 highly salt-sensitive. The regression model for salt tolerance at the seedling stage was defined as: D = - 0.223 + 0.085X1 + 0.203X2 + 0.075X3 + 0.149X6 + 0.132X7 + 0.070X9 + 0.084X10 (R² = 0.969, P < 0.01), identifying relative plant height, leaf area, SPAD value, root fresh and dry weights, SOD activity, and proline content as key indicators. For the full growth stage, the model was: D = - 0.153 + 0.143X1 + 0.443X6 + 0.171X7 (R² = 0.962, P < 0.01), highlighting relative plant height, grain number per plant, and grain weight per plant as critical metrics. This study establishes a comprehensive and systematic framework for evaluating salt tolerance in soybean across different developmental stages and provides a solid technical foundation and valuable germplasm resources for future research on salt tolerance mechanisms and the development of salt-tolerant soybean varieties.

Key words: soybean, germination stage, seedling stage, entire growth stage, salt tolerance, membership function method, comprehensive evaluation

MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm[J].Acta Agronomica Sinica, 2025, 51(8): 1991-2008.

Figures/Tables 16

Table 1

Accession numbers and origins of the soybean germplasm resources"

编号 No.	品种 Variety	产地 Origin	编号 No.	品种 Variety	产地 Origin
1	汾豆33 Fendou 33	山西 Shanxi	26	冀2004 Ji 2004	河北 Hebei
2	阜豆36 Fudou 36	安徽 Anhui	27	齐黄51 Qihuang 51	山东 Shandong
3	潍豆10号 Weidou 10	山东 Shandong	28	汾豆99 Fendou 99	山西 Shanxi
4	齐黄30 Qihuang 30	山东 Shandong	29	汾豆100 Fendou 100	山西 Shanxi
5	石豆5号 Shidou 5	河北 Hebei	30	中黄17 Zhonghuang 17	北京 Beijing
6	汾豆56 Fendou 56	山西 Shanxi	31	皖豆43 Wandou 43	安徽 Anhui
7	冀2006 Ji 2006	河北 Hebei	32	邯豆15 Handou 15	河北 Hebei
8	冀豆22 Jidou 22	河北 Hebei	33	冀豆25 Jidou 25	河北 Hebei
9	潍豆126 Weidou 126	山东 Shandong	34	五星1号 Wuxing 1	河北 Hebei
10	晋遗19 Jinyi 19	山西 Shanxi	35	冀豆18 Jidou 18	河北 Hebei
11	中黄56 Zhonghuang 56	北京 Beijing	36	齐黄34 Qihuang 34	山东 Shandong
12	沧豆13 Cangdou 13	河北 Hebei	37	阜豆30 Fudou 30	安徽 Anhui
13	冀豆30 Jidou 30	河北 Hebei	38	晋豆19 Jindou 19	山西 Shanxi
14	冀豆29 Jidou 29	河北 Hebei	39	齐黄21 Qihuang 21	山东 Shandong
15	冀豆20 Jidou 20	河北 Hebei	40	冀2107 Ji 2107	河北 Hebei
16	中黄80 Zhonghuang 80	北京 Beijing	41	皖豆66 Wandou 66	安徽 Anhui
17	冀2115 Ji 2115	河北 Hebei	42	沧豆11 Cangdou 11	河北 Hebei
18	冀豆16 Jidou 16	河北 Hebei	43	晋遗30 Jinyi 30	山西 Shanxi
19	中黄36 Zhonghuang 36	北京 Beijing	44	冀豆17 Jidou17	河北 Hebei
20	冀2206 Ji 2206	河北 Hebei	45	中黄24 Zhonghuang 24	北京 Beijing
21	石豆14 Shidou 14	河北 Hebei	46	五星4号Wuxing 4	河北 Hebei
22	中黄35 Zhonghuang 35	北京 Beijing	47	汾豆20 Fendou 20	山西 Shanxi
23	冀豆23 Jidou 23	河北 Hebei	48	冀青豆2号 Jiqingdou 2	河北 Hebei
24	冀豆26 Jidou 26	河北 Hebei	49	沧豆18 Cangdou 18	河北 Hebei
25	冀豆12 Jidou 12	河北 Hebei	50	中黄74 Zhonghuang 74	北京 Beijing

Table 1

Table 2

Fig. 1

Table 3

Table 4

Table 5

Table 6

Comprehensive evaluation of salt tolerance in soybean seedlings"

编号 Code	综合指标值 Comprehensive index				隶属函数值 Subordinate function value				D值 D-value	耐盐排序 Salt tolerance ranking	耐盐等级 Salt resistance grade
编号 Code	CI(1)	CI(2)	CI(3)	CI(4)	U(1)	U(2)	U(3)	U(4)	D值 D-value	耐盐排序 Salt tolerance ranking	耐盐等级 Salt resistance grade
1	1.79	1.98	0.32	-0.52	0.70	0.87	0.60	0.33	0.66	8	II级耐盐 Grade II salt tolerance
2	1.27	-1.21	-1.06	-0.78	0.63	0.32	0.35	0.27	0.45	26	III级中间型 Grade III intermediate type
3	-1.64	-1.24	-0.97	-0.66	0.20	0.31	0.37	0.30	0.19	47	V级盐敏感 Grade V salt sensitive
4	1.41	-2.12	0.37	1.03	0.65	0.16	0.61	0.63	0.53	17	III级中间型 Grade III intermediate type
5	-0.01	-3.05	-1.11	0.48	0.44	0.00	0.34	0.52	0.33	42	IV级不耐盐 Grade IV salt intolerance
6	-0.09	-1.08	-0.37	-0.46	0.43	0.34	0.48	0.34	0.40	34	III级中间型 Grade III intermediate type
7	-1.88	-1.29	0.32	-0.54	0.17	0.30	0.60	0.32	0.31	44	IV级不耐盐 Grade IV salt intolerance
8	1.69	0.22	0.68	2.05	0.69	0.56	0.67	0.83	0.68	6	II级耐盐 Grade II salt tolerance
9	2.35	-0.81	-3.02	-0.17	0.79	0.39	0.00	0.39	0.48	21	III级中间型 Grade III intermediate type
10	0.64	-1.20	2.53	-0.05	0.54	0.32	1.00	0.42	0.56	14	III级中间型 Grade III intermediate type
11	1.38	-0.81	0.04	-2.06	0.64	0.39	0.55	0.02	0.47	22	III级中间型 Grade III intermediate type
12	-0.04	2.52	0.61	-0.67	0.44	0.96	0.65	0.30	0.58	13	III级中间型 Grade III intermediate type
13	-2.13	-1.54	2.13	-1.14	0.13	0.26	0.93	0.20	0.33	43	IV级不耐盐 Grade IV salt intolerance
14	-2.29	-0.12	1.14	2.24	0.11	0.51	0.75	0.87	0.44	30	III级中间型 Grade III intermediate type
15	-1.91	-1.02	0.97	2.03	0.16	0.35	0.72	0.83	0.41	33	III级中间型 Grade III intermediate type
16	0.31	-1.53	-0.97	-0.04	0.49	0.26	0.37	0.42	0.40	35	III级中间型 Grade III intermediate type
17	-3.03	0.02	-1.11	-0.89	0.00	0.53	0.34	0.25	0.16	50	V级盐敏感 Grade V salt sensitive
18	-0.35	0.70	0.83	0.00	0.39	0.65	0.69	0.43	0.51	19	III级中间型 Grade III intermediate type
19	-1.43	-0.44	1.44	-0.75	0.23	0.45	0.80	0.28	0.40	36	III级中间型 Grade III intermediate type
20	-1.30	1.33	-0.91	0.75	0.25	0.76	0.38	0.58	0.44	29	III级中间型 Grade III intermediate type
21	1.28	-1.50	-1.55	-0.16	0.63	0.27	0.26	0.40	0.44	28	III级中间型 Grade III intermediate type
22	1.49	1.49	0.07	-0.89	0.66	0.78	0.56	0.25	0.61	11	II级耐盐 Grade II salt tolerance
23	3.25	0.14	-1.24	-1.02	0.92	0.55	0.32	0.23	0.61	9	II级耐盐 Grade II salt tolerance
24	3.63	-0.16	0.80	0.52	0.97	0.50	0.69	0.53	0.74	4	II级耐盐 Grade II salt tolerance
25	2.58	0.28	2.29	1.24	0.82	0.57	0.96	0.67	0.77	1	II级耐盐 Grade II salt tolerance
26	2.02	-1.90	-0.77	0.89	0.74	0.20	0.41	0.60	0.53	16	III级中间型 Grade III intermediate type
27	-1.85	-1.41	1.13	-1.49	0.17	0.28	0.75	0.13	0.30	45	IV级不耐盐 Grade IV salt intolerance
28	-2.24	-1.21	-0.16	-1.36	0.12	0.32	0.52	0.16	0.17	49	V级盐敏感 Grade V salt sensitive
29	-1.59	0.26	1.22	1.13	0.21	0.57	0.76	0.65	0.47	24	III级中间型 Grade III intermediate type
30	-1.98	0.60	0.21	2.90	0.15	0.63	0.58	1.00	0.47	23	III级中间型 Grade III intermediate type
31	-1.33	0.40	-1.07	0.47	0.25	0.59	0.35	0.52	0.39	38	IV级不耐盐 Grade IV salt intolerance
32	-2.12	0.94	-2.44	-0.97	0.13	0.69	0.10	0.24	0.18	48	V级盐敏感 Grade V salt sensitive
33	-0.29	0.15	-1.91	0.99	0.40	0.55	0.20	0.62	0.43	32	III级中间型 Grade III intermediate type
34	-1.64	2.06	-0.88	0.23	0.20	0.88	0.39	0.47	0.43	31	III级中间型 Grade III intermediate type
35	-2.37	2.49	-1.23	0.33	0.10	0.96	0.32	0.49	0.39	37	IV级不耐盐 Grade IV salt intolerance
36	3.80	0.21	0.75	0.32	1.00	0.56	0.68	0.49	0.76	2	II级耐盐 Grade II salt tolerance
37	-1.06	2.75	-0.75	1.09	0.29	1.00	0.41	0.64	0.53	18	III级中间型 Grade III intermediate type
38	-2.04	-1.21	1.37	-2.17	0.14	0.32	0.79	0.00	0.29	46	IV级不耐盐 Grade IV salt intolerance
39	3.82	1.02	-0.10	-0.34	1.00	0.70	0.53	0.36	0.74	3	II级耐盐 Grade II salt tolerance
40	-0.43	1.39	-1.51	-0.40	0.38	0.77	0.27	0.35	0.44	27	III级中间型 Grade III intermediate type
41	-2.15	-0.08	0.55	0.75	0.13	0.51	0.64	0.58	0.38	39	IV级不耐盐 Grade IV salt intolerance
42	0.11	-1.66	-2.62	1.87	0.46	0.24	0.07	0.80	0.38	40	IV级不耐盐 Grade IV salt intolerance
43	0.90	-0.60	-0.03	-0.93	0.57	0.42	0.54	0.24	0.48	20	III级中间型 Grade III intermediate type
44	1.91	1.25	0.56	0.34	0.72	0.74	0.65	0.50	0.68	7	II级耐盐 Grade II salt tolerance
45	-1.42	0.29	-0.44	-1.03	0.24	0.58	0.46	0.22	0.36	41	IV级不耐盐 Grade IV salt intolerance
46	-1.10	-0.85	1.90	0.35	0.28	0.38	0.89	0.50	0.45	25	III级中间型 Grade III intermediate type
47	1.44	1.40	0.21	-0.87	0.65	0.77	0.58	0.26	0.61	12	II级耐盐 Grade II salt tolerance
48	-0.51	2.75	0.80	-1.42	0.37	1.00	0.69	0.15	0.54	15	III级中间型 Grade III intermediate type
49	2.71	0.41	0.99	-0.10	0.84	0.60	0.72	0.41	0.70	5	II级耐盐 Grade II salt tolerance
50	0.45	0.99	2.00	-0.11	0.51	0.70	0.90	0.41	0.61	10	II级耐盐 Grade II salt tolerance

Table 6

Table 7

Table 8

Table 9

Table 10

Comprehensive evaluation of salt tolerance of soybean throughout the entire growth period"

编号 Code	综合指标值 Comprehensive index			隶属函数值 Subordinate function value			D值 D-value	耐盐排序Salt tolerance ranking	耐盐等级 Salt resistance grade
编号 Code	CI(1)	CI(2)	CI(3)	U(1)	U(2)	U(3)	D值 D-value	耐盐排序Salt tolerance ranking	耐盐等级 Salt resistance grade
1	-0.82	-0.54	-1.13	0.30	0.33	0.29	0.31	41	IV级不耐盐 Grade IV salt intolerance
2	-2.87	-1.13	-0.66	0.04	0.23	0.40	0.15	50	V级盐敏感 Grade V salt sensitive
3	-2.07	1.18	-0.02	0.14	0.61	0.56	0.34	39	IV级不耐盐 Grade IV salt intolerance
4	-0.29	1.33	-1.98	0.37	0.63	0.08	0.39	30	IV级不耐盐 Grade IV salt intolerance
5	-1.23	-1.35	1.21	0.25	0.19	0.86	0.34	38	IV级不耐盐 Grade IV salt intolerance
6	-1.85	-0.59	0.19	0.17	0.32	0.61	0.29	44	IV级不耐盐 Grade IV salt intolerance
7	1.14	0.02	-2.24	0.56	0.42	0.02	0.43	26	III级中间型 Grade III intermediate type
8	-1.32	-0.07	-0.79	0.24	0.40	0.37	0.31	42	IV级不耐盐 Grade IV salt intolerance
9	-1.03	0.32	-0.18	0.28	0.47	0.52	0.37	33	IV级不耐盐 Grade IV salt intolerance
10	-0.71	-0.07	-0.22	0.32	0.40	0.51	0.37	32	IV级不耐盐 Grade IV salt intolerance
11	1.10	0.52	-1.54	0.55	0.50	0.19	0.47	18	III级中间型 Grade III intermediate type
12	0.31	-2.11	0.53	0.45	0.07	0.69	0.39	28	IV级不耐盐 Grade IV salt intolerance
13	-0.96	-1.32	1.15	0.28	0.20	0.84	0.36	34	IV级不耐盐 Grade IV salt intolerance
14	2.71	1.04	0.97	0.76	0.58	0.80	0.72	4	II级耐盐 Grade II salt tolerance
15	0.42	1.25	-0.56	0.47	0.62	0.43	0.50	16	III级中间型 Grade III intermediate type
16	-3.14	0.21	-1.21	0.00	0.45	0.27	0.17	49	V级盐敏感 Grade V salt sensitive
17	-1.06	0.09	-1.48	0.27	0.43	0.21	0.30	43	IV级不耐盐 Grade IV salt intolerance
18	-1.27	1.78	0.90	0.24	0.71	0.78	0.46	21	III级中间型 Grade III intermediate type
19	1.63	0.70	-0.48	0.62	0.53	0.45	0.57	8	III级中间型Grade III intermediate type
20	2.06	3.58	0.75	0.68	1.00	0.74	0.77	3	I级高耐盐 Grade I high salt tolerance
21	0.51	0.20	0.24	0.48	0.45	0.62	0.50	17	III级中间型 Grade III intermediate type
22	3.78	0.30	1.55	0.90	0.46	0.94	0.80	2	I级高耐盐 Grade I high salt tolerance
23	-2.62	1.52	0.36	0.07	0.66	0.65	0.33	40	IV级不耐盐 Grade IV salt intolerance
24	-0.88	2.10	-0.63	0.30	0.76	0.41	0.44	25	III级中间型 Grade III intermediate type
25	1.09	-0.88	1.32	0.55	0.27	0.88	0.54	11	III级中间型 Grade III intermediate type
26	-2.05	-1.68	-0.48	0.14	0.14	0.45	0.20	47	V级盐敏感 Grade V salt sensitive
27	-0.26	-0.31	-0.74	0.38	0.36	0.38	0.37	31	IV级不耐盐 Grade IV salt intolerance
28	-0.43	1.06	0.19	0.35	0.59	0.61	0.46	22	III级中间型 Grade III intermediate type
29	0.26	-0.03	1.49	0.44	0.41	0.92	0.52	14	III级中间型 Grade III intermediate type
30	-0.81	1.93	1.79	0.30	0.73	1.00	0.54	10	III级中间型 Grade III intermediate type
31	-2.56	2.38	0.05	0.08	0.80	0.57	0.36	36	IV级不耐盐 Grade IV salt intolerance
32	-0.99	0.25	1.62	0.28	0.45	0.95	0.45	23	III级中间型 Grade III intermediate type
33	4.48	1.47	1.42	1.00	0.65	0.91	0.89	1	I级高耐盐 Grade I high salt tolerance
34	1.42	0.07	0.46	0.60	0.43	0.67	0.57	9	III级中间型Grade III intermediate type
35	2.18	-1.56	-0.21	0.70	0.16	0.51	0.52	13	III级中间型Grade III intermediate type
36	-1.47	-1.09	-0.29	0.22	0.24	0.49	0.27	45	IV级不耐盐Grade IV salt intolerance
37	-0.16	-1.33	-0.42	0.39	0.20	0.46	0.35	37	IV级不耐盐Grade IV salt intolerance
38	3.09	-0.71	-2.33	0.81	0.30	0.00	0.53	12	III级中间型Grade III intermediate type
39	-0.68	2.72	-1.47	0.32	0.86	0.21	0.44	24	III级中间型Grade III intermediate type
40	0.31	-0.79	-0.58	0.45	0.28	0.42	0.40	27	IV级不耐盐Grade IV salt intolerance
41	-3.08	-0.64	0.14	0.01	0.31	0.60	0.19	48	V级盐敏感Grade V salt sensitive
42	1.02	-1.64	0.42	0.54	0.15	0.66	0.46	20	III级中间型Grade III intermediate type
43	0.33	-0.91	1.81	0.45	0.27	1.00	0.50	15	III级中间型Grade III intermediate type
44	4.51	-0.49	-1.61	1.00	0.33	0.17	0.68	5	II级耐盐Grade II salt tolerance
45	2.23	-0.52	-0.02	0.70	0.33	0.56	0.58	7	III级中间型Grade III intermediate type
46	-2.92	-1.74	1.12	0.03	0.13	0.83	0.20	46	V级盐敏感Grade V salt sensitive
47	1.00	-2.53	-1.06	0.54	0.00	0.31	0.36	35	IV级不耐盐Grade IV salt intolerance
48	2.99	-0.82	0.47	0.80	0.28	0.68	0.64	6	II级耐盐Grade II salt tolerance
49	-1.04	-0.66	1.24	0.27	0.31	0.86	0.39	29	IV级不耐盐Grade IV salt intolerance
50	0.02	-0.52	1.13	0.41	0.33	0.84	0.47	19	III级中间型Grade III intermediate type

Table 10

Fig. 2

Table 11

Fig. 3

Table 12

Fig. 4

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级别 Level	相对盐害指数 Relative salt damage index (%)	耐盐性 Salt tolerance
1	0.0-20.0	高耐盐 High salt tolerance
2	20.1-40.0	耐盐 Salt tolerance
3	40.1-60.0	中耐盐 Medium salt tolerance
4	60.1-80.0	盐敏感 Salt sensitive
5	80.1-100.0	盐高感 High salt sensitive

指标 Item	相对株高Relative plant height	相对叶面积Relative leaf area	相对SPAD值 Relative SPAD value	相对地上部分鲜重Relative fresh weight of aboveground parts	相对地上部分干重Relative dry weight of aboveground parts	相对地下部分鲜重Relative fresh weight of underground parts	相对地下部分干重Relative dry weight of underground parts	相对MDA Relative MDA content	相对SOD Relative SOD content	相对PRO Relative PRO content
相对株高 Relative plant height	1.000
相对叶面积 Relative leaf area	0.191	1.000
相对SPAD值 Relative SPAD value	0.642^**	0.165	1.000
相对地上部分鲜重Relative fresh weight of aboveground parts	0.061	0.053	0.042	1.000
相对地上部分干重Relative dry weight of aboveground parts	0.011	0.086	−0.049	0.727^**	1.000
相对地下部分鲜重Relative fresh weight of underground parts	0.155	0.125	0.046	0.010	0.025	1.000
相对地下部分干重Relative dry weight of underground parts	0.001	0.240	−0.025	0.168	0.341^*	0.257	1.000
相对MDA Relative MDA content	−0.132	−0.454^**	−0.106	−0.382^**	−0.351^*	−0.132	−0.192	1.000
相对SOD Relative SOD content	−0.167	−0.565^**	−0.208	−0.243	−0.286	−0.207	−0.193	0.642^**	1.000
相对PRO Relative PRO content	−0.198	−0.615^**	−0.198	−0.236	−0.208	−0.182	−0.213	0.703^**	0.900^**	1.000

指标 Item	主成分因子 Principal component factor
指标 Item	F1	F2	F3	F4
相对株高 Relative plant height	0.511	0.177	0.429	-0.577
相对叶面积 Relative leaf area	0.840	-0.159	-0.133	0.316
相对SPAD值 Relative SPAD value	0.490	0.151	0.480	-0.572
相对地上部分鲜重 Relative fresh weight of aboveground parts	0.180	0.585	-0.646	-0.288
相对地上部分干重 Relative dry weight of aboveground parts	0.146	0.695	-0.597	-0.090
相对地下部分鲜重 Relative fresh weight of underground parts	0.336	0.599	0.483	0.379
相对地下部分干重 Relative underground dry weight	0.318	0.655	0.307	0.407
相对MDA含量 Relative MDA content	-0.704	0.266	0.272	0.182
相对SOD活性 Relative SOD activity	0.888	-0.239	-0.101	0.096
相对PRO含量 Relative PRO content	0.899	-0.275	-0.131	0.179
特征值 Eigenvalue	3.567	1.896	1.635	1.235
贡献率 Contribution ratio (%)	35.674	18.960	16.347	12.350
累积贡献率 Cumulative contribution ratio (%)	35.674	54.634	70.981	83.331

性状 Trait	相对株高Relative plant height	相对底荚高度Relative bottom pod height	相对主茎节数 Relative number of main stem nodes	相对有效分枝Relatively effective branching	相对单株荚数Relative number of pods per plant	相对单株粒数Relative number of grains per plant	相对单株粒重Relative grain weight per plant	相对百粒重Relative weight per hundred grains
相对株高 Relative plant height	1.000
相对底荚高度 Relative bottom pod height	-0.006	1.000
相对主茎节数 Relative number of main stem nodes	0.184	0.149	1.000
相对有效分枝 Relatively effective branching	-0.001	0.773^**	0.093	1.000
相对单株荚数 Relative number of pods per plant	-0.018	0.902^**	0.241	0.876^**	1.000
相对单株粒数 Relative number of grains per plant	0.078	0.920^**	0.244	0.841^**	0.958^**	1.000
相对单株粒重 Relative grain weight per plant	0.134	-0.124	-0.016	-0.099^**	-0.116	-0.143	1.000
相对百粒重 Relative weight per hundred grains	0.015	0.097	-0.079	0.079	0.050	0.072	0.707^**	1.000

指标 Item	主成分因子Principal component factor
指标 Item	F1	F2	F3
相对株高 Relative plant height	0.029	0.136	0.788
相对底荚高度 Relative bottom pod height	0.939	0.050	-0.080
相对主茎节数 Relative number of main stem nodes	0.257	-0.191	0.717
相对有效分枝 Relatively effective branching	0.904	0.060	-0.112
相对单株荚数 Relative number of pods per plant	0.980	0.021	-0.032
相对单株粒数 Relative number of grains per plant	0.979	0.024	0.032
相对单株粒重 Relative grain weight per plant	-0.158	0.898	0.165
相对百粒重 Relative weight per hundred grains	0.045	0.931	-0.115
特征值 Eigenvalue	3.711	1.736	1.198
贡献率 Contribution ratio (%)	46.392	21.699	14.971
累积贡献率 Cumulative contribution ratio (%)	46.392	68.091	83.061

Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm

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耐盐等级 Salt tolerance level	发芽期耐盐大豆 Salt tolerant soybeans during germination stage	苗期耐盐大豆 Salt tolerant soybean during seedling stage	全生育期耐盐大豆 Salt tolerant soybeans throughout their entire growth stage
高耐盐 High salt tolerance	1, 9, 22, 23, 36, 44, 48, 49	—	33, 22, 20
耐盐 Salt tolerance	2, 5, 12, 15, 17, 24, 27, 30, 33, 40	25, 36, 39, 24, 49, 8, 44, 1, 23, 50, 22, 47	14, 44, 48

时期 Stage	发芽期 Germination stage	苗期 Seedling stage	全生育期 Entire growth stage
发芽期 Germination stage	1.000
苗期 Seedling stage	0.175	1.000
全生育期 Entire growth stage	-0.081	0.111	1.000

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